Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Cupriavidus necator |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,4,6-trichlorophenol + FADH2 + O2 | Cupriavidus necator | - |
2,6-dichloro-1,4-benzoquinone + Cl- + FAD + H2O | - |
? | |
2,4,6-trichlorophenol + FADH2 + O2 | Cupriavidus necator JMP134 | - |
2,6-dichloro-1,4-benzoquinone + Cl- + FAD + H2O | - |
? | |
2,6-dichlorohydroquinone + FADH2 + O2 | Cupriavidus necator | - |
2-chloro-6-hydroxy-1,4-benzoquinone + Cl- + FAD + H2O | - |
? | |
2,6-dichlorohydroquinone + FADH2 + O2 | Cupriavidus necator JMP134 | - |
2-chloro-6-hydroxy-1,4-benzoquinone + Cl- + FAD + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cupriavidus necator | Q8KTD8 | - |
- |
Cupriavidus necator JMP134 | Q8KTD8 | - |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate precipitation, Ni-NTA column chromatography, and phenyl agarose column chromatography | Cupriavidus necator |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.0035 | - |
with 2,6-dichlorohydroquinone as substrate, at pH 7.0 and 33°C | Cupriavidus necator |
0.327 | - |
with 2,4,6-trichlorophenol as substrate, at pH 7.0 and 33°C | Cupriavidus necator |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,4,6-trichlorophenol + FADH2 + O2 | - |
Cupriavidus necator | 2,6-dichloro-1,4-benzoquinone + Cl- + FAD + H2O | - |
? | |
2,4,6-trichlorophenol + FADH2 + O2 | - |
Cupriavidus necator JMP134 | 2,6-dichloro-1,4-benzoquinone + Cl- + FAD + H2O | - |
? | |
2,6-dichlorohydroquinone + FADH2 + O2 | - |
Cupriavidus necator | 2-chloro-6-hydroxy-1,4-benzoquinone + Cl- + FAD + H2O | - |
? | |
2,6-dichlorohydroquinone + FADH2 + O2 | - |
Cupriavidus necator JMP134 | 2-chloro-6-hydroxy-1,4-benzoquinone + Cl- + FAD + H2O | - |
? | |
additional information | no activity with riboflavin and flavin mononucleotide instead of FADH2 | Cupriavidus necator | ? | - |
- |
|
additional information | no activity with riboflavin and flavin mononucleotide instead of FADH2 | Cupriavidus necator JMP134 | ? | - |
- |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 60000, SDS-PAGE | Cupriavidus necator |
monomer | 1 * 60792, calculated from amino acid sequence | Cupriavidus necator |
Synonyms | Comment | Organism |
---|---|---|
tcpA | - |
Cupriavidus necator |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
33 | - |
- |
Cupriavidus necator |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
22 | 45 | 66, 88, 85, 86, and 42% of the optimal activity is retained at 22, 27, 30, 37, and 45°C, respectively | Cupriavidus necator |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FADH2 | - |
Cupriavidus necator |