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Literature summary for 1.14.14.173 extracted from

  • Hayes, R.P.; Webb, B.N.; Subramanian, A.K.; Nissen, M.; Popchock, A.; Xun, L.; Kang, C.
    Structural and catalytic differences between two FADH2-dependent monooxygenases 2,4,5-TCP 4-monooxygenase (TftD) from Burkholderia cepacia AC1100 and 2,4,6-TCP 4-monooxygenase (TcpA) from Cupriavidus necator JMP134 (2012), Int. J. Mol. Sci., 13, 9769-9784 .
    View publication on PubMedView publication on EuropePMC
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Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Cupriavidus necator

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging drop vapor diffusion method, using 20% (w/v) polyethylene glycol 3350 (pH 7.4) with 0.2 M potassium sodium tartrate tetrahydrate Cupriavidus necator

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2,4,6-trichlorophenol + FADH2 + O2 Cupriavidus necator
-
 2,6-dichloro-1,4-benzoquinone + Cl- + FAD + H2O
-
 ?
2,4,6-trichlorophenol + FADH2 + O2 Cupriavidus necator JMP134
-
 2,6-dichloro-1,4-benzoquinone + Cl- + FAD + H2O
-
 ?
2,6-dichlorohydroquinone + FADH2 + O2 Cupriavidus necator
-
 2-chloro-6-hydroxy-1,4-benzoquinone + Cl- + FAD + H2O
-
 ?
2,6-dichlorohydroquinone + FADH2 + O2 Cupriavidus necator JMP134
-
 2-chloro-6-hydroxy-1,4-benzoquinone + Cl- + FAD + H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Cupriavidus necator Q8KTD8
-
-
Cupriavidus necator JMP134 Q8KTD8
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography and Mono Q column chromatography Cupriavidus necator

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,4,6-trichlorophenol + FADH2 + O2
-
 Cupriavidus necator 2,6-dichloro-1,4-benzoquinone + Cl- + FAD + H2O
-
 ?
2,4,6-trichlorophenol + FADH2 + O2
-
 Cupriavidus necator JMP134 2,6-dichloro-1,4-benzoquinone + Cl- + FAD + H2O
-
 ?
2,6-dichlorohydroquinone + FADH2 + O2
-
 Cupriavidus necator 2-chloro-6-hydroxy-1,4-benzoquinone + Cl- + FAD + H2O
-
 ?
2,6-dichlorohydroquinone + FADH2 + O2
-
 Cupriavidus necator JMP134 2-chloro-6-hydroxy-1,4-benzoquinone + Cl- + FAD + H2O
-
 ?

Synonyms

Synonyms Comment Organism
2,4,6-TCP 4-monooxygenase
-
 Cupriavidus necator
tcpA
-
 Cupriavidus necator

Cofactor

Cofactor Comment Organism Structure
FADH2
-
 Cupriavidus necator