BRENDA - Enzyme Database
show all sequences of 1.14.14.171

Combinatorial biosynthesis of sapogenins and saponins in Saccharomyces cerevisiae using a C-16alpha hydroxylase from Bupleurum falcatum

Moses, T.; Pollier, J.; Almagro, L.; Buyst, D.; Van Montagu, M.; Pedreño, M.; Martins, J.; Thevelein, J.; Goossens, A.; Proc. Natl. Acad. Sci. USA 111, 1634-1639 (2014)

Data extracted from this reference:

Cloned(Commentary)
Cloned (Commentary)
Organism
expressed in Saccharomyces cerevisiae strain TM48
Bupleurum falcatum
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
beta-amyrin + [reduced NADPH-hemoprotein reductase] + O2
Bupleurum falcatum
-
16alpha-hydroxy-beta-amyrin + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
?
Organism
Organism
UniProt
Commentary
Textmining
Bupleurum falcatum
-
-
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
root
-
Bupleurum falcatum
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
beta-amyrin + [reduced NADPH-hemoprotein reductase] + O2
-
752030
Bupleurum falcatum
16alpha-hydroxy-beta-amyrin + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
additional information
no activity with lupeol
752030
Bupleurum falcatum
?
-
-
-
?
Synonyms
Synonyms
Commentary
Organism
C-16alpha hydroxylase
-
Bupleurum falcatum
CYP716Y1
-
Bupleurum falcatum
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in Saccharomyces cerevisiae strain TM48
Bupleurum falcatum
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
beta-amyrin + [reduced NADPH-hemoprotein reductase] + O2
Bupleurum falcatum
-
16alpha-hydroxy-beta-amyrin + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
?
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
root
-
Bupleurum falcatum
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
beta-amyrin + [reduced NADPH-hemoprotein reductase] + O2
-
752030
Bupleurum falcatum
16alpha-hydroxy-beta-amyrin + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
additional information
no activity with lupeol
752030
Bupleurum falcatum
?
-
-
-
?
Other publictions for EC 1.14.14.171
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
751609
Moses
Unraveling the triterpenoid s ...
Maesa lanceolata
Mol. Plant
8
122-135
2015
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1
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1
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4
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1
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1
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1
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1
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1
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1
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1
1
-
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752030
Moses
Combinatorial biosynthesis of ...
Bupleurum falcatum
Proc. Natl. Acad. Sci. USA
111
1634-1639
2014
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1
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1
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8
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1
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2
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2
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1
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1
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