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Literature summary for 1.14.14.17 extracted from
- Regulation of ginsenoside and phytosterol biosynthesis by RNA interferences of squalene epoxidase gene in Panax ginseng (2009), Phytochemistry, 71, 36-46.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene PgSQE1, DNA and amino acid sequence determination and analysis, phylogenetic analysis | Panax ginseng |
| gene PgSQE2, DNA and amino acid sequence determination and analysis, phylogenetic analysis | Panax ginseng |
| methyl jasmonate treatment suppresses PgSQE2 mRNA expression | Panax ginseng |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | RNA interference of PgSQE1 in transgenic Panax ginseng plants completely suppresses PgSQE1 transcription. Concomitantly, the interference of PgSQE1 results in reduction of ginsenoside production | Panax ginseng |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| additional information | RNA interference of PgSQE1 completely suppresses PgSQE1 transcription and thereby upregulates PgSQE2 expression | Panax ginseng |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| membrane | PgSQE1 contains 4 putative transmembrane helices | Panax ginseng | 16020 | - |
| membrane | PgSQE2 contains 3 putative transmembrane helices | Panax ginseng | 16020 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 59100 | - |
x * 59100, about, sequence calculation | Panax ginseng |
| 60200 | - |
x * 60200, about, sequence calculation | Panax ginseng |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Panax ginseng | - |
- |
- |
| Panax ginseng | B7TWW5 | PgSQE2; gene PgSQE2 | - |
| Panax ginseng | Q75W20 | PgSQE1; gene PgSQE1 | - |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| flower | - |
Panax ginseng | - |
| flower bud | - |
Panax ginseng | - |
| flower bud | PgSQE2 mRNA expression | Panax ginseng | - |
| flower bud | weak expression of PgSQE2 | Panax ginseng | - |
| leaf | - |
Panax ginseng | - |
| additional information | PgSQE1 mRNA abundantly accumulates in all organs. PgSQE1 mRNA accumulates preferentially in vascular bundle tissue and resin ducts of petioles | Panax ginseng | - |
| additional information | PgSQE2 is only weakly expressed and preferentially in petioles and flower buds. PgSQE2 mRNAs accumulates preferentially in vascular bundle tissue and resin ducts of petioles | Panax ginseng | - |
| petiole | - |
Panax ginseng | - |
| petiole | PgSQE2 mRNA expression | Panax ginseng | - |
| petiole | weak expression of PgSQE2 | Panax ginseng | - |
| root | methyl jasmonate treatment enhanced the accumulation of PgSQE1 mRNA | Panax ginseng | - |
| root | fine roots and root body | Panax ginseng | - |
| vascular bundle | PgSQE2 mRNA expression | Panax ginseng | - |
| whole plant | PgSQE1 mRNA abundantly accumulated in all organs | Panax ginseng | - |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 59100, about, sequence calculation | Panax ginseng |
| ? | x * 60200, about, sequence calculation | Panax ginseng |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| PgSQE1 | - |
Panax ginseng |
| PgSQE2 | - |
Panax ginseng |
| SQE | - |
Panax ginseng |
| squalene epoxidase | - |
Panax ginseng |
| squalene epoxidase | coding gene PgSQE1, 537 amino acids | Panax ginseng |
| squalene epoxidase | coding gene PgSQE2, 545 amino acids | Panax ginseng |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FAD | conserved FAD binding sequence motif | Panax ginseng |  |
Expression
| Organism | Comment | Expression |
|---|---|---|
| Panax ginseng | methyl jasmonate treatment suppresses expression of PgSQE2 | down |
| Panax ginseng | squalene treatment coordinately upregulates the expression of PgSQE1, and methyl jasmonate treatment enhances the accumulation of PgSQE1 mRNA in roots. Silencing of PgSQE1 in RNAi roots strongly upregulates PgSQE2 and PNX, cycloartenol synthase, and results in enhanced phytosterol accumulation | up |
| Panax ginseng | squalene treatment coordinately upregulates the expression of PgSQE2. Silencing of PgSQE1 in RNAi roots strongly upregulates PgSQE2 and PNX, cycloartenol synthase, and results in enhanced phytosterol accumulation | up |
General Information
| General Information | Comment | Organism |
|---|---|---|
| metabolism | squalene epoxidase catalyzes the first oxygenation step in phytosterol and triterpenoid saponin biosynthesis and is suggested to represent one of the rate-limiting enzymes in this pathway. Expression of PgSQE1 and PgSQE2 are regulated in a different manner | Panax ginseng |
| metabolism | squalene epoxidase catalyzes the first oxygenation step in phytosterol and triterpenoid saponin biosynthesis and is suggested to represent one of the rate-limiting enzymes in this pathway. Expression of PgSQE1 and PgSQE2 are regulated in a different manner, and that PgSQE1 regulates ginsenoside biosynthesis, but not that of phytosterols in Panax ginseng | Panax ginseng |
| additional information | silencing of PgSQE1 in RNAi roots strongly upregulates PgSQE2 and PNX, cycloartenol synthase, and results in enhanced phytosterol accumulation | Panax ginseng |
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