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Literature summary for 1.14.14.17 extracted from
- Cytochrome P450 17alpha hydroxylase/17,20 lyase (CYP17) function in cholesterol biosynthesis: identification of squalene monooxygenase (epoxidase) activity associated with CYP17 in Leydig cells (2005), Mol. Endocrinol., 19, 1918-1931.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression of His-tagged cytochrome P450 17alpha hydroxylase/17,20 lyase CYP17 in Escherichia coli | Mus musculus |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | knockout of cytochrome P450 17alpha hydroxylase/17,20 lyase CYP17 shows dramatically reduced de novo synthesis of steroids, e.g. progesterone, which can partially be rescued by transfection of CYP17, the latter cells can synthesize progesterone if supplemented with precusors squalene epoxide, lanosterol, zymosterol, and desmosterol, but not squalene | Mus musculus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| additional information | inhibitor SU10603 inhibits the cytochrome P450 17alpha hydroxylase/17,20 lyase activity of CYP17 but not the squalene epoxidase activity | Mus musculus |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| microsome | - |
Mus musculus | - |
- |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Mus musculus | the enzyme activity is associated with cytochrome P450 17alpha hydroxylase/17,20 lyase CYP17 in cholesterol biosynthesis in Leydig cells | ? | - |
? |  |
| squalene + reduced acceptor + O2 | Mus musculus | epoxidation of squalene is the first step in cholesterol biosynthesis | (S)-squalene-2,3-epoxide + acceptor + H2O | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Mus musculus | - |
- |
- |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| squalene + [reduced NADPH-hemoprotein reductase] + O2 = (3S)-2,3-epoxy-2,3-dihydrosqualene + [oxidized NADPH-hemoprotein reductase] + H2O | cytochrome P450 17alpha hydroxylase/17,20 lyase CYP17 also catalyzes the reaction of squalene epoxidase | Mus musculus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| Leydig cell | tumor cell defective in cytochrome P450 17alpha hydroxylase/17,20 lyase activity | Mus musculus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Mus musculus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | the enzyme activity is associated with cytochrome P450 17alpha hydroxylase/17,20 lyase CYP17 in cholesterol biosynthesis in Leydig cells | Mus musculus | ? | - |
? | |
| squalene + reduced acceptor + O2 | - |
Mus musculus | (S)-squalene-2,3-epoxide + acceptor + H2O | - |
? | |
| squalene + reduced acceptor + O2 | epoxidation of squalene is the first step in cholesterol biosynthesis | Mus musculus | (S)-squalene-2,3-epoxide + acceptor + H2O | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 48000-50000 + x * 28000-30000, recombinant His-tagged cytochrome P450 17alpha hydroxylase/17,20 lyase CYP17, SDS-PAGE | Mus musculus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| CYP17 | - |
Mus musculus |
| cytochrome P450 17alpha hydroxylase/17,20 lyase | - |
Mus musculus |
| squalene epoxidase | - |
Mus musculus |
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