BRENDA - Enzyme Database
show all sequences of 1.14.14.168

Lettuce costunolide synthase (CYP71BL2) and its homolog (CYP71BL1) from sunflower catalyze distinct regio- and stereoselective hydroxylations in sesquiterpene lactone metabolism

Ikezawa, N.; Goepfert, J.C.; Nguyen, D.T.; Kim, S.U.; OMaille, P.E.; Spring, O.; Ro, D.K.; J. Biol. Chem. 286, 21601-21611 (2011)

Data extracted from this reference:

Cloned(Commentary)
Cloned (Commentary)
Organism
sunflower HaG8H and LsCOS are coexpressed with Artemisia annua CPR in pESCUra vector
Helianthus annuus
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
germacra-1(10),4,11(13)-trien-12-oate + [reduced NADPH-hemoprotein reductase] + O2
Helianthus annuus
-
8beta-hydroxygermacra-1(10),4,11(13)-trien-12-oate + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
?
germacra-1(10),4,11(13)-trien-12-oate + [reduced NADPH-hemoprotein reductase] + O2
Helianthus annuus cv. HA300
-
8beta-hydroxygermacra-1(10),4,11(13)-trien-12-oate + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
?
Organism
Organism
UniProt
Commentary
Textmining
Helianthus annuus
F8S1H3
-
-
Helianthus annuus cv. HA300
F8S1H3
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
germacra-1(10),4,11(13)-trien-12-oate + [reduced NADPH-hemoprotein reductase] + O2
-
751031
Helianthus annuus
8beta-hydroxygermacra-1(10),4,11(13)-trien-12-oate + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
germacra-1(10),4,11(13)-trien-12-oate + [reduced NADPH-hemoprotein reductase] + O2
germacra-1(10),4,11(13)-trien-12-oate i.e. germacrene A acid
751031
Helianthus annuus
8beta-hydroxygermacra-1(10),4,11(13)-trien-12-oate + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
germacra-1(10),4,11(13)-trien-12-oate + [reduced NADPH-hemoprotein reductase] + O2
-
751031
Helianthus annuus cv. HA300
8beta-hydroxygermacra-1(10),4,11(13)-trien-12-oate + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
germacra-1(10),4,11(13)-trien-12-oate + [reduced NADPH-hemoprotein reductase] + O2
germacra-1(10),4,11(13)-trien-12-oate i.e. germacrene A acid
751031
Helianthus annuus cv. HA300
8beta-hydroxygermacra-1(10),4,11(13)-trien-12-oate + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
Synonyms
Synonyms
Commentary
Organism
CYP71BL1
-
Helianthus annuus
GAA 8x03-hydroxylase
-
Helianthus annuus
HaG8H
-
Helianthus annuus
Cofactor
Cofactor
Commentary
Organism
Structure
cytochrome P-450
heme-thiolate protein
Helianthus annuus
Cloned(Commentary) (protein specific)
Commentary
Organism
sunflower HaG8H and LsCOS are coexpressed with Artemisia annua CPR in pESCUra vector
Helianthus annuus
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
cytochrome P-450
heme-thiolate protein
Helianthus annuus
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
germacra-1(10),4,11(13)-trien-12-oate + [reduced NADPH-hemoprotein reductase] + O2
Helianthus annuus
-
8beta-hydroxygermacra-1(10),4,11(13)-trien-12-oate + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
?
germacra-1(10),4,11(13)-trien-12-oate + [reduced NADPH-hemoprotein reductase] + O2
Helianthus annuus cv. HA300
-
8beta-hydroxygermacra-1(10),4,11(13)-trien-12-oate + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
?
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
germacra-1(10),4,11(13)-trien-12-oate + [reduced NADPH-hemoprotein reductase] + O2
-
751031
Helianthus annuus
8beta-hydroxygermacra-1(10),4,11(13)-trien-12-oate + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
germacra-1(10),4,11(13)-trien-12-oate + [reduced NADPH-hemoprotein reductase] + O2
germacra-1(10),4,11(13)-trien-12-oate i.e. germacrene A acid
751031
Helianthus annuus
8beta-hydroxygermacra-1(10),4,11(13)-trien-12-oate + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
germacra-1(10),4,11(13)-trien-12-oate + [reduced NADPH-hemoprotein reductase] + O2
-
751031
Helianthus annuus cv. HA300
8beta-hydroxygermacra-1(10),4,11(13)-trien-12-oate + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
germacra-1(10),4,11(13)-trien-12-oate + [reduced NADPH-hemoprotein reductase] + O2
germacra-1(10),4,11(13)-trien-12-oate i.e. germacrene A acid
751031
Helianthus annuus cv. HA300
8beta-hydroxygermacra-1(10),4,11(13)-trien-12-oate + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
General Information
General Information
Commentary
Organism
evolution
HaG8H appears to be restricted to the Heliantheae and possibly some other tribes in the Asteroideae subfamily
Helianthus annuus
General Information (protein specific)
General Information
Commentary
Organism
evolution
HaG8H appears to be restricted to the Heliantheae and possibly some other tribes in the Asteroideae subfamily
Helianthus annuus
Other publictions for EC 1.14.14.168
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
749465
Frey
Biosynthesis of eupatolide-A ...
Helianthus annuus
ACS Chem. Biol.
13
1536-1543
2018
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1
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-
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1
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1
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2
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3
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1
-
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1
1
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1
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2
-
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751862
Gou
Discovery of a non-stereosele ...
Inula hupehensis
Plant J.
93
92-106
2018
-
-
1
-
-
-
-
-
-
-
-
1
-
5
-
-
1
-
-
4
-
-
2
-
1
1
-
-
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1
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1
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1
1
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1
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1
-
4
-
-
2
-
1
-
-
-
1
-
-
-
-
1
1
-
-
-
751031
Ikezawa
Lettuce costunolide synthase ...
Helianthus annuus, Helianthus annuus cv. HA300
J. Biol. Chem.
286
21601-21611
2011
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-
1
-
-
-
-
-
-
-
-
2
-
2
-
-
-
-
-
-
-
-
4
-
3
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
1
1
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