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Literature summary for 1.14.14.168 extracted from

  • Frey, M.; Schmauder, K.; Pateraki, I.; Spring, O.
    Biosynthesis of eupatolide-A metabolic route for sesquiterpene lactone formation involving the P450 enzyme CYP71DD6 (2018), ACS Chem. Biol., 13, 1536-1543 .
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
coexpression of HaG8H with the enzyme combination HaGAS1 + HaGAO in Nicotiana benthamiana leaves results in the formation of the lactonized version inunolide Helianthus annuus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
germacra-1(10),4,11(13)-trien-12-oate + [reduced NADPH-hemoprotein reductase] + O2 Helianthus annuus
-
8beta-hydroxygermacra-1(10),4,11(13)-trien-12-oate + [oxidized NADPH-hemoprotein reductase] + H2O
-
?

Organism

Organism UniProt Comment Textmining
Helianthus annuus F8S1H3
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
germacra-1(10),4,11(13)-trien-12-oate + [reduced NADPH-hemoprotein reductase] + O2
-
Helianthus annuus 8beta-hydroxygermacra-1(10),4,11(13)-trien-12-oate + [oxidized NADPH-hemoprotein reductase] + H2O
-
?
germacra-1(10),4,11(13)-trien-12-oate + [reduced NADPH-hemoprotein reductase] + O2 germacra-1(10),4,11(13)-trien-12-oate i.e. germacrene A acid Helianthus annuus 8beta-hydroxygermacra-1(10),4,11(13)-trien-12-oate + [oxidized NADPH-hemoprotein reductase] + H2O
-
?

Synonyms

Synonyms Comment Organism
CYP71BL1
-
Helianthus annuus
GAA 8beta-hydroxylase
-
Helianthus annuus
HaG8H
-
Helianthus annuus

Cofactor

Cofactor Comment Organism Structure
cytochrome P-450 heme-thiolate protein Helianthus annuus