BRENDA - Enzyme Database
show all sequences of 1.14.14.166

The effects of promoter variations of the N-methylcanadine 1-hydroxylase (CYP82Y1) gene on the noscapine production in opium poppy

Abedini, D.; Monfared, S.R.; Abbasi, A.; Sci. Rep. 8, 4973 (2018)

Data extracted from this reference:

Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
(S)-N-methylcanadine + [reduced NADPH-hemoprotein reductase] + O2
Papaver bracteatum
the enzyme participates in the biosynthesis of the isoquinoline alkaloid noscapine
(S)-1-hydroxy-N-methylcanadine + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
?
(S)-N-methylcanadine + [reduced NADPH-hemoprotein reductase] + O2
Papaver somniferum
the enzyme participates in the biosynthesis of the isoquinoline alkaloid noscapine
(S)-1-hydroxy-N-methylcanadine + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
?
Organism
Organism
UniProt
Commentary
Textmining
Papaver bracteatum
-
-
-
Papaver somniferum
I3PLR1
-
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
leaf
-
Papaver somniferum
-
leaf
expression is lower than in Papaver somniferum
Papaver bracteatum
-
seed
-
Papaver somniferum
-
stem
-
Papaver somniferum
-
stem
expression is lower than in Papaver somniferum
Papaver bracteatum
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
(S)-N-methylcanadine + [reduced NADPH-hemoprotein reductase] + O2
-
752243
Papaver bracteatum
(S)-1-hydroxy-N-methylcanadine + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
(S)-N-methylcanadine + [reduced NADPH-hemoprotein reductase] + O2
-
752243
Papaver somniferum
(S)-1-hydroxy-N-methylcanadine + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
(S)-N-methylcanadine + [reduced NADPH-hemoprotein reductase] + O2
the enzyme participates in the biosynthesis of the isoquinoline alkaloid noscapine
752243
Papaver bracteatum
(S)-1-hydroxy-N-methylcanadine + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
(S)-N-methylcanadine + [reduced NADPH-hemoprotein reductase] + O2
the enzyme participates in the biosynthesis of the isoquinoline alkaloid noscapine
752243
Papaver somniferum
(S)-1-hydroxy-N-methylcanadine + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
Synonyms
Synonyms
Commentary
Organism
CYP82Y1
-
Papaver somniferum
N-methylcanadine 1-hydroxylase
-
Papaver bracteatum
N-methylcanadine 1-hydroxylase
-
Papaver somniferum
PbCYP82Y1
-
Papaver bracteatum
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
(S)-N-methylcanadine + [reduced NADPH-hemoprotein reductase] + O2
Papaver bracteatum
the enzyme participates in the biosynthesis of the isoquinoline alkaloid noscapine
(S)-1-hydroxy-N-methylcanadine + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
?
(S)-N-methylcanadine + [reduced NADPH-hemoprotein reductase] + O2
Papaver somniferum
the enzyme participates in the biosynthesis of the isoquinoline alkaloid noscapine
(S)-1-hydroxy-N-methylcanadine + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
?
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
leaf
-
Papaver somniferum
-
leaf
expression is lower than in Papaver somniferum
Papaver bracteatum
-
seed
-
Papaver somniferum
-
stem
-
Papaver somniferum
-
stem
expression is lower than in Papaver somniferum
Papaver bracteatum
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
(S)-N-methylcanadine + [reduced NADPH-hemoprotein reductase] + O2
-
752243
Papaver bracteatum
(S)-1-hydroxy-N-methylcanadine + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
(S)-N-methylcanadine + [reduced NADPH-hemoprotein reductase] + O2
-
752243
Papaver somniferum
(S)-1-hydroxy-N-methylcanadine + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
(S)-N-methylcanadine + [reduced NADPH-hemoprotein reductase] + O2
the enzyme participates in the biosynthesis of the isoquinoline alkaloid noscapine
752243
Papaver bracteatum
(S)-1-hydroxy-N-methylcanadine + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
(S)-N-methylcanadine + [reduced NADPH-hemoprotein reductase] + O2
the enzyme participates in the biosynthesis of the isoquinoline alkaloid noscapine
752243
Papaver somniferum
(S)-1-hydroxy-N-methylcanadine + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
Expression
Organism
Commentary
Expression
Papaver somniferum
an SSR (Simple Sequence Repeats) is embedded in the promoter region of the CYP82Y1 gene. Quantitative PCR shows that a variation in the motif repeat number (either a decrease or increase) down-regulates the expression of the CYP82Y1 gene
down
General Information
General Information
Commentary
Organism
metabolism
the enzyme participates in the biosynthesis of the isoquinoline alkaloid noscapine
Papaver bracteatum
metabolism
the enzyme participates in the biosynthesis of the isoquinoline alkaloid noscapine
Papaver somniferum
General Information (protein specific)
General Information
Commentary
Organism
metabolism
the enzyme participates in the biosynthesis of the isoquinoline alkaloid noscapine
Papaver bracteatum
metabolism
the enzyme participates in the biosynthesis of the isoquinoline alkaloid noscapine
Papaver somniferum
Expression (protein specific)
Organism
Commentary
Expression
Papaver somniferum
an SSR (Simple Sequence Repeats) is embedded in the promoter region of the CYP82Y1 gene. Quantitative PCR shows that a variation in the motif repeat number (either a decrease or increase) down-regulates the expression of the CYP82Y1 gene
down
Other publictions for EC 1.14.14.166
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
749191
Li
Complete biosynthesis of nosc ...
Papaver somniferum
Proc. Natl. Acad. Sci. USA
115
E3922-E3931
2018
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1
1
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752243
Abedini
The effects of promoter varia ...
Papaver bracteatum, Papaver somniferum
Sci. Rep.
8
4973
2018
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5
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2
1
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751038
Dang
CYP82Y1 is N-methylcanadine 1 ...
Papaver somniferum
J. Biol. Chem.
289
2013-2026
2014
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6
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