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Literature summary for 1.14.14.163 extracted from

  • Li, Y.; Li, S.; Thodey, K.; Trenchard, I.; Cravens, A.; Smolke, C.
    Complete biosynthesis of noscapine and halogenated alkaloids in yeast (2018), Proc. Natl. Acad. Sci. USA, 115, E3922-E3931 .
    View publication on PubMedView publication on EuropePMC

Application

Application Comment Organism
synthesis de novo production of noscapine in Saccharomyces cerevisiae, through the reconstruction of a biosynthetic pathway comprising over 30 enzymes from plants, bacteria, mammals, and yeast itself, including 7 plant endoplasmic reticulum (ER)-localized enzymes. Optimization directed to tuning expression of pathway enzymes, host endogenous metabolic pathways, and fermentation conditions led to an over 18,000-fold improvement from initial noscapine titers to 2.2 mg/l Papaver somniferum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(S)-1-hydroxy-N-methylcanadine + [reduced NADPH-hemoprotein reductase] + O2 Papaver somniferum the enzyme participates in the biosynthesis of the isoquinoline alkaloid noscapine (13S,14R)-1,13-dihydroxy-N-methylcanadine + [oxidized NADPH-hemoprotein reductase] + H2O
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Organism

Organism UniProt Comment Textmining
Papaver somniferum I3PLR0
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(S)-1-hydroxy-N-methylcanadine + [reduced NADPH-hemoprotein reductase] + O2
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Papaver somniferum (13S,14R)-1,13-dihydroxy-N-methylcanadine + [oxidized NADPH-hemoprotein reductase] + H2O
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?
(S)-1-hydroxy-N-methylcanadine + [reduced NADPH-hemoprotein reductase] + O2 the enzyme participates in the biosynthesis of the isoquinoline alkaloid noscapine Papaver somniferum (13S,14R)-1,13-dihydroxy-N-methylcanadine + [oxidized NADPH-hemoprotein reductase] + H2O
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?

Synonyms

Synonyms Comment Organism
1-hydroxy-N-methylcanadine 13-hydroxylase
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Papaver somniferum
CYP82X2
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Papaver somniferum

General Information

General Information Comment Organism
metabolism the enzyme participates in the biosynthesis of the isoquinoline alkaloid noscapine Papaver somniferum