Application | Comment | Organism |
---|---|---|
synthesis | de novo production of noscapine in Saccharomyces cerevisiae, through the reconstruction of a biosynthetic pathway comprising over 30 enzymes from plants, bacteria, mammals, and yeast itself, including 7 plant endoplasmic reticulum (ER)-localized enzymes. Optimization directed to tuning expression of pathway enzymes, host endogenous metabolic pathways, and fermentation conditions led to an over 18,000-fold improvement from initial noscapine titers to 2.2 mg/l | Papaver somniferum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-1-hydroxy-N-methylcanadine + [reduced NADPH-hemoprotein reductase] + O2 | Papaver somniferum | the enzyme participates in the biosynthesis of the isoquinoline alkaloid noscapine | (13S,14R)-1,13-dihydroxy-N-methylcanadine + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Papaver somniferum | I3PLR0 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-1-hydroxy-N-methylcanadine + [reduced NADPH-hemoprotein reductase] + O2 | - |
Papaver somniferum | (13S,14R)-1,13-dihydroxy-N-methylcanadine + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
(S)-1-hydroxy-N-methylcanadine + [reduced NADPH-hemoprotein reductase] + O2 | the enzyme participates in the biosynthesis of the isoquinoline alkaloid noscapine | Papaver somniferum | (13S,14R)-1,13-dihydroxy-N-methylcanadine + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
1-hydroxy-N-methylcanadine 13-hydroxylase | - |
Papaver somniferum |
CYP82X2 | - |
Papaver somniferum |
General Information | Comment | Organism |
---|---|---|
metabolism | the enzyme participates in the biosynthesis of the isoquinoline alkaloid noscapine | Papaver somniferum |