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Literature summary for 1.14.14.162 extracted from

  • Vanegas, K.G.; Larsen, A.B.; Eichenberger, M.; Fischer, D.; Mortensen, U.H.; Naesby, M.
    Indirect and direct routes to C-glycosylated flavones in Saccharomyces cerevisiae (2018), Microb. Cell Fact., 17, 107 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.14.14.162

Application

Application Comment Organism
synthesis two-step indirect glycosylation using combinations of flavanone 2-hydroxylase and C-glycosyltransferases, to convert 2-hydroxyflavanone intermediates into the 6C-glucoside flavones isovitexin and isoorientin, and the 8C-glucoside flavones vitexin and orientin. The ratio between 6C and 8C glycosylation depended on the C-glycosyltransferase used. The indirect route resuls in mixtures, similar to what has been reported for in vitro experiments Oryza sativa Japonica Group

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
eriodictyol + [reduced NADPH-hemoprotein reductase] + O2 Oryza sativa Japonica Group
-
 2-hydroxyeriodictyol + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
naringenin + [reduced NADPH-hemoprotein reductase] + O2 Oryza sativa Japonica Group
-
 2-hydroxynaringenin + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Oryza sativa Japonica Group Q5VRI5
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
eriodictyol + [reduced NADPH-hemoprotein reductase] + O2
-
 Oryza sativa Japonica Group 2-hydroxyeriodictyol + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
naringenin + [reduced NADPH-hemoprotein reductase] + O2
-
 Oryza sativa Japonica Group 2-hydroxynaringenin + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?

Synonyms

Synonyms Comment Organism
CYP93G2
-
 Oryza sativa Japonica Group
OsF2H
-
 Oryza sativa Japonica Group

Cofactor

Cofactor Comment Organism Structure
cytochrome P-450 a cytochrome P-450 (heme thiolate) enzyme Oryza sativa Japonica Group