Cloned (Comment) | Organism |
---|---|
CYP Ge-5/CYP93B1, expression in Sf9 insect cells and in Saccharomyces cerevisiae BJ2168 | Glycyrrhiza echinata |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
microsome | recombinant enzyme, expressed in Sf9 insect cells or yeast cells | Glycyrrhiza echinata | - |
- |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Glycyrrhiza echinata | flavone biosynthesis | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Glycyrrhiza echinata | P93149 | - |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
a flavanone + [reduced NADPH-hemoprotein reductase] + O2 = a 2-hydroxyflavanone + [oxidized NADPH-hemoprotein reductase] + H2O | mechanism, stereoselectivity | Glycyrrhiza echinata |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2S)-liquiritigenin + [reduced NADPH-hemoprotein reductase] + O2 | hydroxylation of liquiritigenin at C-2 and formation of licodione as a result of non-enzymatic hemiacetal opening, selectivity towards (2S)-flavanone | Glycyrrhiza echinata | licodione + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
(2S)-naringenin + [reduced NADPH-hemoprotein reductase] + O2 | selectivity towards (2S)-flavanone | Glycyrrhiza echinata | 2-hydroxynaringenin + [oxidized NADPH-hemoprotein reductase] + H2O | the product is further converted into apigenin when treated with HCl | ? | |
eriodictyol + [reduced NADPH-hemoprotein reductase] + O2 | - |
Glycyrrhiza echinata | luteolin + [oxidized NADPH-hemoprotein reductase] + H2O | product is formed after acid treatment | ? | |
additional information | flavone biosynthesis | Glycyrrhiza echinata | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
(2S)-flavanone 2-hydroxylase | - |
Glycyrrhiza echinata |
F2H | - |
Glycyrrhiza echinata |
LS | - |
Glycyrrhiza echinata |
More | CYP93B1 encodes (2S)-flavanone 2-hydroxylase, which has previously been designated to licodione synthase and flavone synthase II | Glycyrrhiza echinata |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Glycyrrhiza echinata |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
cytochrome P450 | - |
Glycyrrhiza echinata |