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Literature summary for 1.14.14.16 extracted from
- Human cytochrome P450 21A2, the major steroid 21-hydroxylase: structure of the enzyme-progesterone substrate complex and rate-limiting C-H bond cleavage (2015), J. Biol. Chem., 290, 13128-13143.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
- |
Bos taurus |
| expressed in Escherichia coli BL21-Gold DE(3) cells | Homo sapiens |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| in complex with progesterone, sitting drop vapor diffusion method, using 0.1 M sodium HEPES (pH 7.5) containing 0.2 M ammonium sulfate and 25% (w/v) polyethylene glycol 3350 | Homo sapiens |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00021 | - |
progesterone | recombinant enzyme, at pH 7.4 and 37°C | Homo sapiens |  |
| 0.00044 | - |
17alpha-hydroxyprogesterone | recombinant enzyme, at pH 7.4 and 37°C | Bos taurus | |
| 0.0005 | - |
progesterone | recombinant enzyme, at pH 7.4 and 37°C | Bos taurus | |
| 0.0015 | - |
17alpha-hydroxyprogesterone | recombinant enzyme, at pH 7.4 and 37°C | Homo sapiens | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 50000 | - |
x * 50000, SDS-PAGE | Homo sapiens |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 17alpha-hydroxyprogesterone + [reduced NADPH-hemoprotein reductase] + O2 | Bos taurus | - |
11-deoxycortisol + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| 17alpha-hydroxyprogesterone + [reduced NADPH-hemoprotein reductase] + O2 | Homo sapiens | - |
11-deoxycortisol + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| progesterone + [reduced NADPH-hemoprotein reductase] + O2 | Bos taurus | - |
11-deoxycorticosterone + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| progesterone + [reduced NADPH-hemoprotein reductase] + O2 | Homo sapiens | - |
11-deoxycorticosterone + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bos taurus | P00191 | - |
- |
| Homo sapiens | P08686 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Bos taurus |
| Ni-NTA agarose column chromatography, and SP Fast-Flow Sepharose column chromatography | Homo sapiens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| adrenal cortex | - |
Bos taurus | - |
| adrenal cortex | - |
Homo sapiens | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 17alpha-hydroxyprogesterone + [reduced NADPH-hemoprotein reductase] + O2 | - |
Bos taurus | 11-deoxycortisol + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| 17alpha-hydroxyprogesterone + [reduced NADPH-hemoprotein reductase] + O2 | - |
Homo sapiens | 11-deoxycortisol + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| progesterone + [reduced NADPH-hemoprotein reductase] + O2 | - |
Bos taurus | 11-deoxycorticosterone + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| progesterone + [reduced NADPH-hemoprotein reductase] + O2 | - |
Homo sapiens | 11-deoxycorticosterone + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 50000, SDS-PAGE | Homo sapiens |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| CYP21A2 | - |
Bos taurus |
| CYP21A2 | - |
Homo sapiens |
| cytochrome p450 21A2 | - |
Bos taurus |
| cytochrome p450 21A2 | - |
Homo sapiens |
| Steroid 21-hydroxylase | - |
Bos taurus |
| Steroid 21-hydroxylase | - |
Homo sapiens |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.078 | - |
progesterone | recombinant enzyme, at pH 7.4 and 37°C | Bos taurus | |
| 0.67 | - |
17alpha-hydroxyprogesterone | recombinant enzyme, at pH 7.4 and 37°C | Bos taurus | |
| 2.8 | - |
progesterone | recombinant enzyme, at pH 7.4 and 37°C | Homo sapiens | |
| 4 | - |
17alpha-hydroxyprogesterone | recombinant enzyme, at pH 7.4 and 37°C | Homo sapiens | |
General Information
| General Information | Comment | Organism |
|---|---|---|
| malfunction | deficiency of this enzyme is involved in about 95% of cases of human congenital adrenal hyperplasia | Bos taurus |
| malfunction | deficiency of this enzyme is involved in about 95% of cases of human congenital adrenal hyperplasia | Homo sapiens |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1517 | - |
progesterone | recombinant enzyme, at pH 7.4 and 37°C | Bos taurus | |
| 1567 | - |
17alpha-hydroxyprogesterone | recombinant enzyme, at pH 7.4 and 37°C | Bos taurus | |
| 2667 | - |
17alpha-hydroxyprogesterone | recombinant enzyme, at pH 7.4 and 37°C | Homo sapiens | |
| 13330 | - |
progesterone | recombinant enzyme, at pH 7.4 and 37°C | Homo sapiens | |
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Release 2023.1 (January 2023)
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