Crystallization (Comment) | Organism |
---|---|
plate shaped crystals from 4.0 M sodium formate and 28% PEG 8000 in 0.2 M sodium acetate, X-ray analysis | Pseudomonas putida |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
additional information | - |
enzyme consists of 2 components: 1 oxygenating and 1 NADH dehydrogenase | Pseudomonas putida |
36000 | - |
NADH dehydrogenase component, SDS-PAGE | Pseudomonas putida |
38000 | - |
oxygenating component, gel filtration | Pseudomonas putida |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(-)-bornane-2,5-dione + FMNH2 + O2 | Pseudomonas putida | inducible by growth on racemic camphor | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas putida | - |
- |
- |
Pseudomonas putida NCIMB 10007 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
both components | Pseudomonas putida |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
(-)-bornane-2,5-dione + reduced rubredoxin + O2 + FMNH2 = (-)-5-oxo-1,2-campholide + FMN + H2O | mechanism | Pseudomonas putida | |
(-)-bornane-2,5-dione + reduced rubredoxin + O2 + FMNH2 = (-)-5-oxo-1,2-campholide + FMN + H2O | belongs to group of Bayer-Villiger monooxygenases of the NADH plus FMN-dependent type 2 enzymes | Pseudomonas putida |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(-)-bornane-2,5-dione + FMNH2 + O2 | 6-dione i.e. 3,6-diketocamphane | Pseudomonas putida | 1,8,8-trimethyl-2-oxabicyclo[3.2.1]octane-3,6-dione + FMN + H2O | i.e. cyclopentaneacetic acid, 3-hydroxy-2,2,3-trimethyl-5-oxo, delta-lactone, i.e. 3,4,4-trimethyl-6-carboxy-methyl-DELTA3-cyclopentenone, product is an unstable lactone-intermediate and forms spontaneously (2,2-dimethyl-5-oxo-cyclopent-3-en-1-yl)acetic acid, i.e. 2-oxo-DELTA3-4,5,5-trimethylcyclopentenyl acetic acid | ? | |
(-)-bornane-2,5-dione + FMNH2 + O2 | inducible by growth on racemic camphor | Pseudomonas putida | ? | - |
? | |
(-)-bornanone + FMNH2 + O2 | i.e. (-)-camphor | Pseudomonas putida | ? | - |
? | |
additional information | rubredoxin not mentioned | Pseudomonas putida | ? | - |
? | |
additional information | rubredoxin not mentioned | Pseudomonas putida NCIMB 10007 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 38000, oxygenating component, SDS-PAGE | Pseudomonas putida |
Synonyms | Comment | Organism |
---|---|---|
3,6-diketocamphane-monooxygenase | - |
Pseudomonas putida |