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Literature summary for 1.14.14.155 extracted from
- Diketocamphane enantiomer-specific 'Baeyer-Villiger' monooxygenases from camphor-grown Pseudomonas putida ATCC 17453 (1993), J. Gen. Microbiol., 139, 797-805.
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytoplasm | NADH dehydrogenase component forming a loosely complex with the oxygenating component | Pseudomonas putida | 5737 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| additional information | - |
enzyme consists of 2 components: 1 oxygenating and 1 NADH dehydrogenase | Pseudomonas putida |
| additional information | - |
enzyme components form a very loose complex | Pseudomonas putida |
| 40300 | - |
oxygenating component, native PAGE | Pseudomonas putida |
| 76000 | - |
both components, ultracentrifugal analysis | Pseudomonas putida |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas putida | - |
inducible by (-)-camphor | - |
| Pseudomonas putida | - |
strain ATCC 17453 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| oxygenating component | Pseudomonas putida |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.244 | - |
substrate (-)-camphor | Pseudomonas putida |
| 300 | - |
purified enzyme component NADH dehydrogenase | Pseudomonas putida |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (-)-bornanone + FMNH2 + O2 | i.e. (-)-camphor | Pseudomonas putida | ? | - |
? |  |
| additional information | enzyme is associated with a NADH oxidase | Pseudomonas putida | ? | - |
? | |
| additional information | rubredoxin not mentioned | Pseudomonas putida | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 40300, oxygenating component, SDS-PAGE | Pseudomonas putida |
| More | enzyme consists of 2 components: 1 oxygenating and 1 NADH dehydrogenase | Pseudomonas putida |
| More | enzyme components from a very loose complex | Pseudomonas putida |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| 3,6-diketocamphane 1,6-monooxygenase | different enzyme with similar biochemical properties, immunological cross-reactivity | Pseudomonas putida |
| 3,6-diketocamphane 1,6-monooxygenase | isoform, which is probably a different enzyme | Pseudomonas putida |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FMN | binds reversibly | Pseudomonas putida | |
| additional information | absolute specificity | Pseudomonas putida | |
| additional information | interaction of inducible NADH dehydrogenase component via bound FMN with oxygenating component | Pseudomonas putida | |
| additional information | FAD is ineffective | Pseudomonas putida | |
| additional information | purified NADH dehydrogenase component from 2,5-diketocamphane-1,2-monooxygenase can be used as electron donor | Pseudomonas putida |
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