Application | Comment | Organism |
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synthesis | production of useful chiral synthons for chemoenzymatic synthesis | Pseudomonas putida |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
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(-)-bornane-2,5-dione + FMNH2 + O2 | Pseudomonas putida | inducible by growth on racemic camphor | ? | - |
? | |
additional information | Pseudomonas putida | catalyzes stereoselective electrophilic biooxidation of a wide range of prochiral organic sulfoxides to the corresponding chiral sulfoxides as well as the nucleophilic biooxidation of ketones to lactones with different enantio- and stereoselectivity, overview | ? | - |
? |
Organism | UniProt | Comment | Textmining |
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Pseudomonas putida | - |
- |
- |
Pseudomonas putida | - |
2 isozymes termed 2,5-diketocamphane-1,2-monooxygenase and 3,6-diketocamphane-1,6-monooxygenase | - |
Pseudomonas putida NCIMB 10007 | - |
- |
- |
Purification (Comment) | Organism |
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- |
Pseudomonas putida |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
(-)-bornane-2,5-dione + reduced rubredoxin + O2 + FMNH2 = (-)-5-oxo-1,2-campholide + FMN + H2O | mechanism | Pseudomonas putida | |
(-)-bornane-2,5-dione + reduced rubredoxin + O2 + FMNH2 = (-)-5-oxo-1,2-campholide + FMN + H2O | catalysis of 2 mechanistically different types of biochemical reactions within the confines of the same active site | Pseudomonas putida | |
(-)-bornane-2,5-dione + reduced rubredoxin + O2 + FMNH2 = (-)-5-oxo-1,2-campholide + FMN + H2O | belongs to group of Bayer-Villiger monooxygenases of the NADH plus FMN-dependent type 2 enzymes | Pseudomonas putida | |
(-)-bornane-2,5-dione + reduced rubredoxin + O2 + FMNH2 = (-)-5-oxo-1,2-campholide + FMN + H2O | cubic space active site model, topography | Pseudomonas putida | |
(-)-bornane-2,5-dione + reduced rubredoxin + O2 + FMNH2 = (-)-5-oxo-1,2-campholide + FMN + H2O | isozyme, enantiocomplementary and isofunctional isozymes 2,5-DKCMO EC 1.14.15.2 and 3,6-DKCMO EC 1.14.15.x | Pseudomonas putida | |
(-)-bornane-2,5-dione + reduced rubredoxin + O2 + FMNH2 = (-)-5-oxo-1,2-campholide + FMN + H2O | higher activity in oxidation of sulfides to the corresponding sulfoxides than in lactonization of ketones | Pseudomonas putida |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(-)-bornane-2,5-dione + FMNH2 + O2 | 6-dione i.e. 3,6-diketocamphane | Pseudomonas putida | 1,8,8-trimethyl-2-oxabicyclo[3.2.1]octane-3,6-dione + FMN + H2O | - |
? | |
(-)-bornane-2,5-dione + FMNH2 + O2 | inducible by growth on racemic camphor | Pseudomonas putida | ? | - |
? | |
(-)-bornanone + FMNH2 + O2 | i.e. (-)-camphor | Pseudomonas putida | ? | - |
? | |
additional information | enzyme is associated with a NADH oxidase | Pseudomonas putida | ? | - |
? | |
additional information | rubredoxin not mentioned | Pseudomonas putida | ? | - |
? | |
additional information | catalyzes stereoselective electrophilic biooxidation of a wide range of prochiral organic sulfoxides to the corresponding chiral sulfoxides as well as the nucleophilic biooxidation of ketones to lactones with different enantio- and stereoselectivity, overview | Pseudomonas putida | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
3,6-diketocamphane 1,6-monooxygenase | isozyme, enantiocomplementary and isofunctional isozymes 2,5-DKCMO EC 1.14.15.2 and 3,6-DKCMO EC 1.14.15.x | Pseudomonas putida |
3,6-diketocamphane 1,6-monooxygenase | i.e. 3,6-DKCMO | Pseudomonas putida |
3,6-diketocamphane 1,6-monooxygenase | isoform, which is probably a different enzyme | Pseudomonas putida |
3,6-diketocamphane-monooxygenase | - |
Pseudomonas putida |