Literature summary for 1.14.14.154 extracted from

Martel, C.M.; Parker, J.E.; Warrilow, A.G.; Rolley, N.J.; Kelly, S.L.; Kelly, D.E.
Complementation of a Saccharomyces cerevisiae ERG11/CYP51 (sterol 14alpha-demethylase) doxycycline-regulated mutant and screening of the azole sensitivity of Aspergillus fumigatus isoenzymes CYP51A and CYP51B (2010), Antimicrob. Agents Chemother., 54, 4920-4923.

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Cloned(Commentary)

Cloned (Comment)	Organism
expression of isoenzymes CYP51A and CYP51B in the Saccharomyces cerevisiae mutant strain YUG37-erg11, wherein native ERG11/CYP51 expression is controlled using a doxycycline-regulatable promoter. Aspergillus fumigatus CYP51A and CYP51B both complement Saccharomyces cerevisiae sterol 14alpha-demethylase function with comparable efficiency. In the presence of doxycycline, recombinant YUG37-pcyp51A and YUG37-pcyp51B yeasts are able to synthesize ergosterol and grow, a control strain harboring reverse-oriented cyp51A cannot. YUG37-pcyp51A and YUG37-pcyp51B constructs show identical sensitivity to itraconazole, posaconazole, clotrimazole, voriconazole, and fluconazole	Aspergillus fumigatus

Cloned (Comment)

Organism

expression of isoenzymes CYP51A and CYP51B in the Saccharomyces cerevisiae mutant strain YUG37-erg11, wherein native ERG11/CYP51 expression is controlled using a doxycycline-regulatable promoter. Aspergillus fumigatus CYP51A and CYP51B both complement Saccharomyces cerevisiae sterol 14alpha-demethylase function with comparable efficiency. In the presence of doxycycline, recombinant YUG37-pcyp51A and YUG37-pcyp51B yeasts are able to synthesize ergosterol and grow, a control strain harboring reverse-oriented cyp51A cannot. YUG37-pcyp51A and YUG37-pcyp51B constructs show identical sensitivity to itraconazole, posaconazole, clotrimazole, voriconazole, and fluconazole

Aspergillus fumigatus

Organism

Organism	UniProt	Comment	Textmining
Aspergillus fumigatus	-	isoenzymes CYP51A and CYP51B	-

Synonyms

Synonyms	Comment	Organism
Cyp51A	-	Aspergillus fumigatus
CYP51B	-	Aspergillus fumigatus
sterol 14alpha-demethylase	-	Aspergillus fumigatus

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Release 2023.1 (January 2023)