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Literature summary for 1.14.14.154 extracted from

  • Warrilow, A.G.; Melo, N.; Martel, C.M.; Parker, J.E.; Nes, W.D.; Kelly, S.L.; Kelly, D.E.
    Expression, purification, and characterization of Aspergillus fumigatus sterol 14-alpha demethylase (CYP51) isoenzymes A and B (2010), Antimicrob. Agents Chemother., 54, 4225-4234.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
CYP51A and CYP51B, sequence comparisons and phylogenetic tree of fungal CYP51 proteins, expression of isozymes CYP51A and CYP51B in Escherichia coli Aspergillus fumigatus

General Stability

General Stability Organism
the dithionite-reduced CO-P450 complex for purified recombinant CYP51A is unstable, rapidly denaturing to inactive P420, in marked contrast to purified recombinant CYP51B, where the CO-P450 complex is stable Aspergillus fumigatus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information the binding of azoles, with the exception of fluconazole, to CYP51B is tight, with the Kd values for clotrimazole, itraconazole, posaconazole, and voriconazole being 0.00021, 0.00006, 0.00012, and 0.00042 mM, respectively, and 0.004 m for fluconazole Aspergillus fumigatus
0.0031
-
lanosterol isozyme CYP51A, pH not specified in the publication, temperature not specified in the publication Aspergillus fumigatus
0.0041
-
eburicol isozyme CYP51A, pH not specified in the publication, temperature not specified in the publication Aspergillus fumigatus
0.0086
-
lanosterol isozyme CYP51B, pH not specified in the publication, temperature not specified in the publication Aspergillus fumigatus
0.0226
-
eburicol isozyme CYP51B, pH not specified in the publication, temperature not specified in the publication Aspergillus fumigatus

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
Aspergillus fumigatus 16020
-

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ heme protein Aspergillus fumigatus

Organism

Organism UniProt Comment Textmining
Aspergillus fumigatus
-
isozymes CYP51A and CYP51B, i.e. AF51A and AF51B
-
Aspergillus fumigatus Af293
-
isozymes CYP51A and CYP51B, i.e. AF51A and AF51B
-

Purification (Commentary)

Purification (Comment) Organism
recombinant isozymes CYP51A and CYP51B from Escherichia coli, the dithionite-reduced CO-P450 complex for CYP51A is unstable, rapidly denaturing to inactive P420, in marked contrast to CYP51B, where the CO-P450 complex is stable Aspergillus fumigatus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
eburicol + [reduced NADPH-hemoprotein reductase] + O2 isozymes CYP51A and CYP51B Aspergillus fumigatus ?
-
?
eburicol + [reduced NADPH-hemoprotein reductase] + O2 isozymes CYP51A and CYP51B Aspergillus fumigatus Af293 ?
-
?
lanosterol + [reduced NADPH-hemoprotein reductase] + O2 isozymes CYP51A and CYP51B Aspergillus fumigatus ?
-
?
lanosterol + [reduced NADPH-hemoprotein reductase] + O2 isozymes CYP51A and CYP51B Aspergillus fumigatus Af293 ?
-
?
additional information azole binding properties of purified CYP51A and CYP51B, overview Aspergillus fumigatus ?
-
?
additional information azole binding properties of purified CYP51A and CYP51B, overview Aspergillus fumigatus Af293 ?
-
?

Synonyms

Synonyms Comment Organism
CYP51
-
Aspergillus fumigatus
Cyp51A
-
Aspergillus fumigatus
CYP51B
-
Aspergillus fumigatus
sterol 14-alpha demethylase
-
Aspergillus fumigatus

Cofactor

Cofactor Comment Organism Structure
heme two distinct azole binding conformations within the heme prosthetic group of CYP51A Aspergillus fumigatus

General Information

General Information Comment Organism
additional information an intrinsic azole fluconazole resistance is observed in Aspergillus fumigatus Aspergillus fumigatus