Cloned (Comment) | Organism |
---|---|
CYP51A and CYP51B, sequence comparisons and phylogenetic tree of fungal CYP51 proteins, expression of isozymes CYP51A and CYP51B in Escherichia coli | Aspergillus fumigatus |
General Stability | Organism |
---|---|
the dithionite-reduced CO-P450 complex for purified recombinant CYP51A is unstable, rapidly denaturing to inactive P420, in marked contrast to purified recombinant CYP51B, where the CO-P450 complex is stable | Aspergillus fumigatus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | the binding of azoles, with the exception of fluconazole, to CYP51B is tight, with the Kd values for clotrimazole, itraconazole, posaconazole, and voriconazole being 0.00021, 0.00006, 0.00012, and 0.00042 mM, respectively, and 0.004 m for fluconazole | Aspergillus fumigatus | |
0.0031 | - |
lanosterol | isozyme CYP51A, pH not specified in the publication, temperature not specified in the publication | Aspergillus fumigatus | |
0.0041 | - |
eburicol | isozyme CYP51A, pH not specified in the publication, temperature not specified in the publication | Aspergillus fumigatus | |
0.0086 | - |
lanosterol | isozyme CYP51B, pH not specified in the publication, temperature not specified in the publication | Aspergillus fumigatus | |
0.0226 | - |
eburicol | isozyme CYP51B, pH not specified in the publication, temperature not specified in the publication | Aspergillus fumigatus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | - |
Aspergillus fumigatus | 16020 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | heme protein | Aspergillus fumigatus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus fumigatus | - |
isozymes CYP51A and CYP51B, i.e. AF51A and AF51B | - |
Aspergillus fumigatus Af293 | - |
isozymes CYP51A and CYP51B, i.e. AF51A and AF51B | - |
Purification (Comment) | Organism |
---|---|
recombinant isozymes CYP51A and CYP51B from Escherichia coli, the dithionite-reduced CO-P450 complex for CYP51A is unstable, rapidly denaturing to inactive P420, in marked contrast to CYP51B, where the CO-P450 complex is stable | Aspergillus fumigatus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
eburicol + [reduced NADPH-hemoprotein reductase] + O2 | isozymes CYP51A and CYP51B | Aspergillus fumigatus | ? | - |
? | |
eburicol + [reduced NADPH-hemoprotein reductase] + O2 | isozymes CYP51A and CYP51B | Aspergillus fumigatus Af293 | ? | - |
? | |
lanosterol + [reduced NADPH-hemoprotein reductase] + O2 | isozymes CYP51A and CYP51B | Aspergillus fumigatus | ? | - |
? | |
lanosterol + [reduced NADPH-hemoprotein reductase] + O2 | isozymes CYP51A and CYP51B | Aspergillus fumigatus Af293 | ? | - |
? | |
additional information | azole binding properties of purified CYP51A and CYP51B, overview | Aspergillus fumigatus | ? | - |
? | |
additional information | azole binding properties of purified CYP51A and CYP51B, overview | Aspergillus fumigatus Af293 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
CYP51 | - |
Aspergillus fumigatus |
Cyp51A | - |
Aspergillus fumigatus |
CYP51B | - |
Aspergillus fumigatus |
sterol 14-alpha demethylase | - |
Aspergillus fumigatus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
heme | two distinct azole binding conformations within the heme prosthetic group of CYP51A | Aspergillus fumigatus |
General Information | Comment | Organism |
---|---|---|
additional information | an intrinsic azole fluconazole resistance is observed in Aspergillus fumigatus | Aspergillus fumigatus |