Cloned (Comment) | Organism |
---|---|
CYP51 cDNA cloned with pBluescript KS-. Self sufficient lanosterol 14-demethylase fusion proteins by using yeast CYP51 and P450 reductase, expressed in Escherichia coli | Saccharomyces cerevisiae |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ketoconazole | - |
Saccharomyces cerevisiae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.004 | - |
lanosterol | fusion protein CYP51 (F1) consisting of the ORF of modified CYP51, a linker region containing the amino acid sequence S-T, and DELTA41 P450 reductase | Saccharomyces cerevisiae | |
0.0044 | - |
lanosterol | fusion protein CYP51 (F2) consisting of the ORF of modified CYP51, a linker region containing the amino acid sequence S-T-E-Q-S-A-K-K-V-R-K-K-A, and DELTA55 P450 reductase | Saccharomyces cerevisiae | |
0.005 | - |
lanosterol | ORF of modified CYP51 | Saccharomyces cerevisiae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | P10614 | - |
- |
Purification (Comment) | Organism |
---|---|
to homogeneity | Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
lanosterol + NADPH + O2 | artificial fused enzymes have comparable activity to the reconstituted system. Reduction of CYP51 both in the fused enzyme and the reconstituted system is biphasic and consisted of an initial fast phase followed by a slow phase | Saccharomyces cerevisiae | 4,4-dimethylcholesta-8,14,24-trien-3-ol + NADP+ + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
CYP51 | - |
Saccharomyces cerevisiae |
lanosterol 14-demethylase | - |
Saccharomyces cerevisiae |