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Literature summary for 1.14.14.154 extracted from

  • Pietila, M.P.; Vohra, P.K.; Sanyal, B.; Wengenack, N.L.; Raghavakaimal, S.; Thomas, C.F.
    Cloning and characterization of CYP51 from Mycobacterium avium (2006), Am. J. Respir. Cell Mol. Biol., 35, 236-242.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.14.14.154

Application

Application Comment Organism
medicine the enzyme is a target in treatment of the chronic lung disease caused by Mycobacterium avium Mycobacterium avium subsp. avium

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli as GST-tagged enzyme Mycobacterium avium subsp. avium

Inhibitors

Inhibitors Comment Organism Structure
econazole
-
 Mycobacterium avium subsp. avium
fluconazole
-
 Mycobacterium avium subsp. avium
itraconazole
-
 Mycobacterium avium subsp. avium
ketoconazole
-
 Mycobacterium avium subsp. avium
voriconazole
-
 Mycobacterium avium subsp. avium

Metals/Ions

Metals/Ions Comment Organism Structure
Iron
-
 Mycobacterium avium subsp. avium

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
lanosterol + [reduced NADPH-hemoprotein reductase] + O2 Mycobacterium avium subsp. avium
-
 ?
-
 ?
obtusifoliol + [reduced NADPH-hemoprotein reductase] + O2 Mycobacterium avium subsp. avium preferred substrate 4alpha-methyl-5alpha-ergost-8,14,24(28)-trien-3beta-ol + formate + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Mycobacterium avium subsp. avium Q3HTM1
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant GST-tagged enzyme from Escherichia coli by glutathione affinity chromatography, cleavage of the tag by thrombin, and thrombin elimination by benzamidine affinity chromatography Mycobacterium avium subsp. avium

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
lanosterol + [reduced NADPH-hemoprotein reductase] + O2
-
 Mycobacterium avium subsp. avium ?
-
 ?
obtusifoliol + [reduced NADPH-hemoprotein reductase] + O2 preferred substrate Mycobacterium avium subsp. avium 4alpha-methyl-5alpha-ergost-8,14,24(28)-trien-3beta-ol + formate + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?

Synonyms

Synonyms Comment Organism
CYP51
-
 Mycobacterium avium subsp. avium
lanosterol 14alpha-demethylase
-
 Mycobacterium avium subsp. avium

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Mycobacterium avium subsp. avium

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
 assay at Mycobacterium avium subsp. avium

Cofactor

Cofactor Comment Organism Structure
Ferredoxin
-
 Mycobacterium avium subsp. avium