Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli with tetrahistidine tag | Streptomyces coelicolor |
Protein Variants | Comment | Organism |
---|---|---|
additional information | mutant A3(2) containing a transposon insertion, no synthesis of functional hemoprotein, viable strain | Streptomyces coelicolor |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces coelicolor | - |
expression in Escherichia coli with tetrahistidine tag | - |
Purification (Comment) | Organism |
---|---|
recombinant enzyme | Streptomyces coelicolor |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
24-methylene-24,25-dihydrolanosterol + [reduced NADPH-hemoprotein reductase] + O2 | - |
Streptomyces coelicolor | 14alpha-demethyl-24-methylene-4alpha-methyl-5alpha-ergosta-8,14,24-trien-3beta-ol + formate + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
additional information | no substrate: lanosterol | Streptomyces coelicolor | ? | - |
? |