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Literature summary for 1.14.14.154 extracted from
- Sterol 14-demethylase P450 (P45014DM*) is one of the most ancient and conserved P450 species (1996), J. Biochem., 119, 926-933.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| cDNA cloning | Homo sapiens |
| cDNA expression in COS7 cells and cloning of pRT-9 cDNA | Rattus norvegicus |
| cDNA, cloning and functional expression | Rattus norvegicus |
| pRT-9 cDNA | Rattus norvegicus |
| pRT-9 clone, pRT-9 protein is P45014DM | Rattus norvegicus |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| microsome | - |
Rattus norvegicus | - |
- |
| microsome | - |
Saccharomyces cerevisiae | - |
- |
| microsome | - |
Embryophyta | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| additional information | - |
enzyme consisting of 509 amino acids, amino acid sequencing | Homo sapiens |
| additional information | - |
protein consisting of 503 amino acids, amino acid sequencing | Rattus norvegicus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| lanosterol + [reduced NADPH-hemoprotein reductase] + O2 | Homo sapiens | lanosta-8,24-dien-3beta-ol | 4,4-dimethyl-5alpha-cholesta-8,14,24-trien-3beta-ol + formate + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? |  |
| lanosterol + [reduced NADPH-hemoprotein reductase] + O2 | Rattus norvegicus | lanosta-8,24-dien-3beta-ol | 4,4-dimethyl-5alpha-cholesta-8,14,24-trien-3beta-ol + formate + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| lanosterol + [reduced NADPH-hemoprotein reductase] + O2 | Saccharomyces cerevisiae | lanosta-8,24-dien-3beta-ol | 4,4-dimethyl-5alpha-cholesta-8,14,24-trien-3beta-ol + formate + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| lanosterol + [reduced NADPH-hemoprotein reductase] + O2 | Homo sapiens | 4,4,14alpha-trimethyl-5alpha-cholesta-8,24-dien-3beta-ol | 4,4-dimethyl-5alpha-cholesta-8,14,24-trien-3beta-ol + formate + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| lanosterol + [reduced NADPH-hemoprotein reductase] + O2 | Rattus norvegicus | 4,4,14alpha-trimethyl-5alpha-cholesta-8,24-dien-3beta-ol | 4,4-dimethyl-5alpha-cholesta-8,14,24-trien-3beta-ol + formate + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| lanosterol + [reduced NADPH-hemoprotein reductase] + O2 | Saccharomyces cerevisiae | 4,4,14alpha-trimethyl-5alpha-cholesta-8,24-dien-3beta-ol | 4,4-dimethyl-5alpha-cholesta-8,14,24-trien-3beta-ol + formate + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| additional information | Homo sapiens | housekeeping enzyme essential for viability of mammals, essential step in cholesterol biosynthesis | ? | - |
? | |
| additional information | Rattus norvegicus | housekeeping enzyme essential for viability of mammals, essential step in cholesterol biosynthesis | ? | - |
? | |
| additional information | Homo sapiens | enzyme of sterol biosynthesis, sterol 14-demethylation occurs in all organism exhibiting de novo sterol biosynthesis | ? | - |
? | |
| additional information | Rattus norvegicus | enzyme of sterol biosynthesis, sterol 14-demethylation occurs in all organism exhibiting de novo sterol biosynthesis | ? | - |
? | |
| additional information | Saccharomyces cerevisiae | enzyme of sterol biosynthesis, sterol 14-demethylation occurs in all organism exhibiting de novo sterol biosynthesis | ? | - |
? | |
| additional information | Schizosaccharomyces pombe | enzyme of sterol biosynthesis, sterol 14-demethylation occurs in all organism exhibiting de novo sterol biosynthesis | ? | - |
? | |
| additional information | Candida albicans | enzyme of sterol biosynthesis, sterol 14-demethylation occurs in all organism exhibiting de novo sterol biosynthesis | ? | - |
? | |
| additional information | Candida tropicalis | enzyme of sterol biosynthesis, sterol 14-demethylation occurs in all organism exhibiting de novo sterol biosynthesis | ? | - |
? | |
| additional information | Ustilago maydis | enzyme of sterol biosynthesis, sterol 14-demethylation occurs in all organism exhibiting de novo sterol biosynthesis | ? | - |
? | |
| additional information | Penicillium italicum | enzyme of sterol biosynthesis, sterol 14-demethylation occurs in all organism exhibiting de novo sterol biosynthesis | ? | - |
? | |
| additional information | Homo sapiens | enzyme of cholesterol biosynthesis | ? | - |
? | |
| additional information | Rattus norvegicus | enzyme of cholesterol biosynthesis | ? | - |
? | |
| additional information | Homo sapiens | there is a possibility that P45014DM participates not only in sterol biogenesis but also in production of biosignal substance regulating meiosis of mammalian oocytes | ? | - |
? | |
| additional information | Rattus norvegicus | there is a possibility that P45014DM participates not only in sterol biogenesis but also in production of biosignal substance regulating meiosis of mammalian oocytes | ? | - |
? | |
| additional information | Homo sapiens | enzyme of sterol biosynthetic pathway | ? | - |
? | |
| additional information | Rattus norvegicus | enzyme of sterol biosynthetic pathway | ? | - |
? | |
| additional information | Saccharomyces cerevisiae | enzyme of sterol biosynthetic pathway | ? | - |
? | |
| additional information | Schizosaccharomyces pombe | enzyme of sterol biosynthetic pathway | ? | - |
? | |
| additional information | Embryophyta | enzyme of sterol biosynthetic pathway | ? | - |
? | |
| additional information | Candida albicans | enzyme of sterol biosynthetic pathway | ? | - |
? | |
| additional information | Candida tropicalis | enzyme of sterol biosynthetic pathway | ? | - |
? | |
| additional information | Ustilago maydis | enzyme of sterol biosynthetic pathway | ? | - |
? | |
| additional information | Penicillium italicum | enzyme of sterol biosynthetic pathway | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Candida albicans | - |
- |
- |
| Candida tropicalis | - |
- |
- |
| Embryophyta | - |
higher plants | - |
| Homo sapiens | - |
human | - |
| Penicillium italicum | - |
- |
- |
| Rattus norvegicus | - |
male rat | - |
| Saccharomyces cerevisiae | - |
- |
- |
| Schizosaccharomyces pombe | - |
- |
- |
| Ustilago maydis | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Rattus norvegicus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | - |
Homo sapiens | - |
| liver | - |
Rattus norvegicus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| lanosterol + [reduced NADPH-hemoprotein reductase] + O2 | lanosta-8,24-dien-3beta-ol | Homo sapiens | 4,4-dimethyl-5alpha-cholesta-8,14,24-trien-3beta-ol + formate + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| lanosterol + [reduced NADPH-hemoprotein reductase] + O2 | lanosta-8,24-dien-3beta-ol | Rattus norvegicus | 4,4-dimethyl-5alpha-cholesta-8,14,24-trien-3beta-ol + formate + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| lanosterol + [reduced NADPH-hemoprotein reductase] + O2 | lanosta-8,24-dien-3beta-ol | Saccharomyces cerevisiae | 4,4-dimethyl-5alpha-cholesta-8,14,24-trien-3beta-ol + formate + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| lanosterol + [reduced NADPH-hemoprotein reductase] + O2 | lanosta-8,24-dien-3beta-ol | Candida albicans | 4,4-dimethyl-5alpha-cholesta-8,14,24-trien-3beta-ol + formate + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| lanosterol + [reduced NADPH-hemoprotein reductase] + O2 | lanosta-8,24-dien-3beta-ol | Candida tropicalis | 4,4-dimethyl-5alpha-cholesta-8,14,24-trien-3beta-ol + formate + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| lanosterol + [reduced NADPH-hemoprotein reductase] + O2 | 4,4,14alpha-trimethyl-5alpha-cholesta-8,24-dien-3beta-ol | Homo sapiens | 4,4-dimethyl-5alpha-cholesta-8,14,24-trien-3beta-ol + formate + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| lanosterol + [reduced NADPH-hemoprotein reductase] + O2 | 4,4,14alpha-trimethyl-5alpha-cholesta-8,24-dien-3beta-ol | Rattus norvegicus | 4,4-dimethyl-5alpha-cholesta-8,14,24-trien-3beta-ol + formate + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| lanosterol + [reduced NADPH-hemoprotein reductase] + O2 | 4,4,14alpha-trimethyl-5alpha-cholesta-8,24-dien-3beta-ol | Saccharomyces cerevisiae | 4,4-dimethyl-5alpha-cholesta-8,14,24-trien-3beta-ol + formate + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| lanosterol + [reduced NADPH-hemoprotein reductase] + O2 | 4,4,14alpha-trimethyl-5alpha-cholesta-8,24-dien-3beta-ol | Candida albicans | 4,4-dimethyl-5alpha-cholesta-8,14,24-trien-3beta-ol + formate + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| lanosterol + [reduced NADPH-hemoprotein reductase] + O2 | 4,4,14alpha-trimethyl-5alpha-cholesta-8,24-dien-3beta-ol | Candida tropicalis | 4,4-dimethyl-5alpha-cholesta-8,14,24-trien-3beta-ol + formate + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| additional information | housekeeping enzyme essential for viability of mammals, essential step in cholesterol biosynthesis | Homo sapiens | ? | - |
? | |
| additional information | housekeeping enzyme essential for viability of mammals, essential step in cholesterol biosynthesis | Rattus norvegicus | ? | - |
? | |
| additional information | enzyme of sterol biosynthesis, sterol 14-demethylation occurs in all organism exhibiting de novo sterol biosynthesis | Homo sapiens | ? | - |
? | |
| additional information | enzyme of sterol biosynthesis, sterol 14-demethylation occurs in all organism exhibiting de novo sterol biosynthesis | Rattus norvegicus | ? | - |
? | |
| additional information | enzyme of sterol biosynthesis, sterol 14-demethylation occurs in all organism exhibiting de novo sterol biosynthesis | Saccharomyces cerevisiae | ? | - |
? | |
| additional information | enzyme of sterol biosynthesis, sterol 14-demethylation occurs in all organism exhibiting de novo sterol biosynthesis | Schizosaccharomyces pombe | ? | - |
? | |
| additional information | enzyme of sterol biosynthesis, sterol 14-demethylation occurs in all organism exhibiting de novo sterol biosynthesis | Candida albicans | ? | - |
? | |
| additional information | enzyme of sterol biosynthesis, sterol 14-demethylation occurs in all organism exhibiting de novo sterol biosynthesis | Candida tropicalis | ? | - |
? | |
| additional information | enzyme of sterol biosynthesis, sterol 14-demethylation occurs in all organism exhibiting de novo sterol biosynthesis | Ustilago maydis | ? | - |
? | |
| additional information | enzyme of sterol biosynthesis, sterol 14-demethylation occurs in all organism exhibiting de novo sterol biosynthesis | Penicillium italicum | ? | - |
? | |
| additional information | enzyme of cholesterol biosynthesis | Homo sapiens | ? | - |
? | |
| additional information | enzyme of cholesterol biosynthesis | Rattus norvegicus | ? | - |
? | |
| additional information | there is a possibility that P45014DM participates not only in sterol biogenesis but also in production of biosignal substance regulating meiosis of mammalian oocytes | Homo sapiens | ? | - |
? | |
| additional information | there is a possibility that P45014DM participates not only in sterol biogenesis but also in production of biosignal substance regulating meiosis of mammalian oocytes | Rattus norvegicus | ? | - |
? | |
| additional information | enzyme of sterol biosynthetic pathway | Homo sapiens | ? | - |
? | |
| additional information | enzyme of sterol biosynthetic pathway | Rattus norvegicus | ? | - |
? | |
| additional information | enzyme of sterol biosynthetic pathway | Saccharomyces cerevisiae | ? | - |
? | |
| additional information | enzyme of sterol biosynthetic pathway | Schizosaccharomyces pombe | ? | - |
? | |
| additional information | enzyme of sterol biosynthetic pathway | Embryophyta | ? | - |
? | |
| additional information | enzyme of sterol biosynthetic pathway | Candida albicans | ? | - |
? | |
| additional information | enzyme of sterol biosynthetic pathway | Candida tropicalis | ? | - |
? | |
| additional information | enzyme of sterol biosynthetic pathway | Ustilago maydis | ? | - |
? | |
| additional information | enzyme of sterol biosynthetic pathway | Penicillium italicum | ? | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Rattus norvegicus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | - |
Rattus norvegicus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| cytochrome P450 | - |
Homo sapiens | |
| cytochrome P450 | - |
Rattus norvegicus | |
| cytochrome P450 | - |
Saccharomyces cerevisiae | |
| cytochrome P450 | - |
Schizosaccharomyces pombe | |
| cytochrome P450 | - |
Embryophyta | |
| cytochrome P450 | - |
Candida albicans | |
| cytochrome P450 | - |
Candida tropicalis | |
| cytochrome P450 | - |
Ustilago maydis | |
| cytochrome P450 | - |
Penicillium italicum | |
| heme | heme-thiolate enzyme | Homo sapiens | |
| heme | heme-thiolate enzyme | Rattus norvegicus | |
| heme | heme-thiolate enzyme | Saccharomyces cerevisiae | |
| heme | heme-thiolate enzyme | Schizosaccharomyces pombe | |
| heme | heme-thiolate enzyme | Embryophyta | |
| heme | heme-thiolate enzyme | Candida albicans | |
| heme | heme-thiolate enzyme | Candida tropicalis | |
| heme | heme-thiolate enzyme | Ustilago maydis | |
| heme | heme-thiolate enzyme | Penicillium italicum | |
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