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Literature summary for 1.14.14.154 extracted from
- Different substrate specificities of lanosterol 14alpha-demethylase (P-45014DM) of Saccharomyces cerevisiae and rat liver for 24-methylene-24,25-dihydrolanosterol and 24,25-dihydrolanosterol (1991), Biochem. Biophys. Res. Commun., 178, 1064-1071.
Application
| Application | Comment | Organism |
|---|---|---|
| agriculture | target enzyme for azole antifungal agents. These specific inhibitors are of great importance as plant growth regulators, fungicides and herbicides in the agricultural and medical fields | Saccharomyces cerevisiae |
| medicine | - |
Rattus norvegicus |
| medicine | target enzyme for azole antifungal agents. These specific inhibitors are of great importance as plant growth regulators, fungicides and herbicides in the agricultural and medical fields | Saccharomyces cerevisiae |
| pharmacology | - |
Rattus norvegicus |
| pharmacology | target enzyme for azole antifungal agents. These specific inhibitors are of great importance as plant growth regulators, fungicides and herbicides in the agricultural and medical fields | Saccharomyces cerevisiae |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 24,25-dihydrolanosterol | 8.3% inhibition of 24-methylene-24,25-dihydrolanosterol demethylation, no inhibition of lanosterol demethylation | Saccharomyces cerevisiae |  |
| 24-methylene-24,25-dihydrolanosterol | 21.6% inhibition of lanosterol demethylation, 55.3% inhibition of 24,25-dihydrolanosterol demethylation | Saccharomyces cerevisiae | |
| azole antifungal agents | specific inhibitors | Rattus norvegicus | |
| azole antifungal agents | specific inhibitors | Saccharomyces cerevisiae | |
| lanosterol | 63% inhibition of 24-methylene-24,25-dihydrolanosterol demethylation, 74.9% inhibition of 24,25-dihydrolanosterol demethylation | Saccharomyces cerevisiae | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0067 | - |
lanosterol | - |
Saccharomyces cerevisiae | |
| 0.0087 | - |
24-methylene-24,25-dihydrolanosterol | - |
Saccharomyces cerevisiae | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytoplasm | - |
Rattus norvegicus | 5737 | - |
| microsome | - |
Rattus norvegicus | - |
- |
| microsome | - |
Saccharomyces cerevisiae | - |
- |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 24,25-dihydrolanosterol + [reduced NADPH-hemoprotein reductase] + O2 | Rattus norvegicus | 8-lanosta-3beta-ol | 4,4-dimethyl-5alpha-cholesta-8,14-dien-3beta-ol + formate + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| 24,25-dihydrolanosterol + [reduced NADPH-hemoprotein reductase] + O2 | Saccharomyces cerevisiae | 8-lanosta-3beta-ol | 4,4-dimethyl-5alpha-cholesta-8,14-dien-3beta-ol + formate + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| 24,25-dihydrolanosterol + [reduced NADPH-hemoprotein reductase] + O2 | Rattus norvegicus | DHL | 4,4-dimethyl-5alpha-cholesta-8,14-dien-3beta-ol + formate + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| 24,25-dihydrolanosterol + [reduced NADPH-hemoprotein reductase] + O2 | Saccharomyces cerevisiae | DHL | 4,4-dimethyl-5alpha-cholesta-8,14-dien-3beta-ol + formate + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| 24,25-dihydrolanosterol + [reduced NADPH-hemoprotein reductase] + O2 | Rattus norvegicus | 8-lanosten-3beta-ol | 4,4-dimethyl-5alpha-cholesta-8,14-dien-3beta-ol + formate + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| 24,25-dihydrolanosterol + [reduced NADPH-hemoprotein reductase] + O2 | Saccharomyces cerevisiae | 8-lanosten-3beta-ol | 4,4-dimethyl-5alpha-cholesta-8,14-dien-3beta-ol + formate + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| 24,25-dihydrolanosterol + [reduced NADPH-hemoprotein reductase] + O2 | Rattus norvegicus | 4,4,14alpha-trimethyl-5alpha-cholesta-8-en-3beta-ol | 4,4-dimethyl-5alpha-cholesta-8,14-dien-3beta-ol + formate + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| 24,25-dihydrolanosterol + [reduced NADPH-hemoprotein reductase] + O2 | Saccharomyces cerevisiae | 4,4,14alpha-trimethyl-5alpha-cholesta-8-en-3beta-ol | 4,4-dimethyl-5alpha-cholesta-8,14-dien-3beta-ol + formate + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| 24,25-dihydrolanosterol + [reduced NADPH-hemoprotein reductase] + O2 | Saccharomyces cerevisiae | not the natural substrate | 4,4-dimethyl-5alpha-cholesta-8,14-dien-3beta-ol + formate + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| lanosterol + [reduced NADPH-hemoprotein reductase] + O2 | Rattus norvegicus | natural substrate | 4,4-dimethyl-5alpha-cholesta-8,14,24-trien-3beta-ol + formate + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| lanosterol + [reduced NADPH-hemoprotein reductase] + O2 | Saccharomyces cerevisiae | natural substrate | 4,4-dimethyl-5alpha-cholesta-8,14,24-trien-3beta-ol + formate + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| lanosterol + [reduced NADPH-hemoprotein reductase] + O2 | Rattus norvegicus | lanosta-8,24-dien-3beta-ol | 4,4-dimethyl-5alpha-cholesta-8,14,24-trien-3beta-ol + formate + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| lanosterol + [reduced NADPH-hemoprotein reductase] + O2 | Saccharomyces cerevisiae | lanosta-8,24-dien-3beta-ol | 4,4-dimethyl-5alpha-cholesta-8,14,24-trien-3beta-ol + formate + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| lanosterol + [reduced NADPH-hemoprotein reductase] + O2 | Rattus norvegicus | 4,4,14alpha-trimethyl-5alpha-cholesta-8,24-dien-3beta-ol | 4,4-dimethyl-5alpha-cholesta-8,14,24-trien-3beta-ol + formate + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| lanosterol + [reduced NADPH-hemoprotein reductase] + O2 | Saccharomyces cerevisiae | 4,4,14alpha-trimethyl-5alpha-cholesta-8,24-dien-3beta-ol | 4,4-dimethyl-5alpha-cholesta-8,14,24-trien-3beta-ol + formate + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| additional information | Rattus norvegicus | removal of 14alpha-methyl group, C32, from 14alpha-methylated precursor sterols is an essential step of sterol biosynthesis in eukaryotes | ? | - |
? | |
| additional information | Saccharomyces cerevisiae | removal of 14alpha-methyl group, C32, from 14alpha-methylated precursor sterols is an essential step of sterol biosynthesis in eukaryotes | ? | - |
? | |
| additional information | Rattus norvegicus | enzyme of cholesterol biosynthesis | ? | - |
? | |
| additional information | Saccharomyces cerevisiae | enzyme of ergosterol biosynthesis | ? | - |
? | |
| additional information | Rattus norvegicus | enzyme of sterol biosynthetic pathway | ? | - |
? | |
| additional information | Saccharomyces cerevisiae | enzyme of sterol biosynthetic pathway | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rattus norvegicus | - |
male sprague-dawley rats | - |
| Saccharomyces cerevisiae | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Rattus norvegicus |
- |
Saccharomyces cerevisiae |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | - |
Rattus norvegicus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Rattus norvegicus |
| additional information | - |
- |
Saccharomyces cerevisiae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 24,25-dihydrolanosterol + [reduced NADPH-hemoprotein reductase] + O2 | 8-lanosta-3beta-ol | Rattus norvegicus | 4,4-dimethyl-5alpha-cholesta-8,14-dien-3beta-ol + formate + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| 24,25-dihydrolanosterol + [reduced NADPH-hemoprotein reductase] + O2 | 8-lanosta-3beta-ol | Saccharomyces cerevisiae | 4,4-dimethyl-5alpha-cholesta-8,14-dien-3beta-ol + formate + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| 24,25-dihydrolanosterol + [reduced NADPH-hemoprotein reductase] + O2 | DHL | Rattus norvegicus | 4,4-dimethyl-5alpha-cholesta-8,14-dien-3beta-ol + formate + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| 24,25-dihydrolanosterol + [reduced NADPH-hemoprotein reductase] + O2 | DHL | Saccharomyces cerevisiae | 4,4-dimethyl-5alpha-cholesta-8,14-dien-3beta-ol + formate + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| 24,25-dihydrolanosterol + [reduced NADPH-hemoprotein reductase] + O2 | 8-lanosten-3beta-ol | Rattus norvegicus | 4,4-dimethyl-5alpha-cholesta-8,14-dien-3beta-ol + formate + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| 24,25-dihydrolanosterol + [reduced NADPH-hemoprotein reductase] + O2 | 8-lanosten-3beta-ol | Saccharomyces cerevisiae | 4,4-dimethyl-5alpha-cholesta-8,14-dien-3beta-ol + formate + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| 24,25-dihydrolanosterol + [reduced NADPH-hemoprotein reductase] + O2 | 4,4,14alpha-trimethyl-5alpha-cholesta-8-en-3beta-ol | Rattus norvegicus | 4,4-dimethyl-5alpha-cholesta-8,14-dien-3beta-ol + formate + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| 24,25-dihydrolanosterol + [reduced NADPH-hemoprotein reductase] + O2 | 4,4,14alpha-trimethyl-5alpha-cholesta-8-en-3beta-ol | Saccharomyces cerevisiae | 4,4-dimethyl-5alpha-cholesta-8,14-dien-3beta-ol + formate + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| 24,25-dihydrolanosterol + [reduced NADPH-hemoprotein reductase] + O2 | not the natural substrate | Saccharomyces cerevisiae | 4,4-dimethyl-5alpha-cholesta-8,14-dien-3beta-ol + formate + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| 24-methylene-24,25-dihydrolanosterol + [reduced NADPH-hemoprotein reductase] + O2 | poorest substrate, catalyzes 14alpha-demethylation of 24-methylene-DHL, but activity is considerably lower than that for lanosterol and for 24,25-dihydrolanosterol, DHL | Rattus norvegicus | 4,4-dimethyl-ergosta-8,14,24(28)-trien-3beta-ol + formate + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| 24-methylene-24,25-dihydrolanosterol + [reduced NADPH-hemoprotein reductase] + O2 | 4,4,14alpha-trimethylergosta-8,24(28)-dien-3beta-ol | Rattus norvegicus | 4,4-dimethyl-ergosta-8,14,24(28)-trien-3beta-ol + formate + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| 24-methylene-24,25-dihydrolanosterol + [reduced NADPH-hemoprotein reductase] + O2 | 4,4,14alpha-trimethylergosta-8,24(28)-dien-3beta-ol | Saccharomyces cerevisiae | 4,4-dimethyl-ergosta-8,14,24(28)-trien-3beta-ol + formate + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| 24-methylene-24,25-dihydrolanosterol + [reduced NADPH-hemoprotein reductase] + O2 | activity for 24-methylene-DHL is considerably higher, 4fold, than that for 24,25-dihydrolanosterol, DHL | Saccharomyces cerevisiae | 4,4-dimethyl-ergosta-8,14,24(28)-trien-3beta-ol + formate + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| 24-methylene-24,25-dihydrolanosterol + [reduced NADPH-hemoprotein reductase] + O2 | 24-methylenelanost-8-en-3beta-ol, 24-methylene-DHL | Rattus norvegicus | 4,4-dimethyl-ergosta-8,14,24(28)-trien-3beta-ol + formate + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| 24-methylene-24,25-dihydrolanosterol + [reduced NADPH-hemoprotein reductase] + O2 | 24-methylenelanost-8-en-3beta-ol, 24-methylene-DHL | Saccharomyces cerevisiae | 4,4-dimethyl-ergosta-8,14,24(28)-trien-3beta-ol + formate + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| 24-methylene-24,25-dihydrolanosterol + [reduced NADPH-hemoprotein reductase] + O2 | about 60% activity to that of lanosterol | Saccharomyces cerevisiae | 4,4-dimethyl-ergosta-8,14,24(28)-trien-3beta-ol + formate + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| lanosterol + [reduced NADPH-hemoprotein reductase] + O2 | best substrate | Rattus norvegicus | 4,4-dimethyl-5alpha-cholesta-8,14,24-trien-3beta-ol + formate + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| lanosterol + [reduced NADPH-hemoprotein reductase] + O2 | best substrate | Saccharomyces cerevisiae | 4,4-dimethyl-5alpha-cholesta-8,14,24-trien-3beta-ol + formate + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| lanosterol + [reduced NADPH-hemoprotein reductase] + O2 | natural substrate | Rattus norvegicus | 4,4-dimethyl-5alpha-cholesta-8,14,24-trien-3beta-ol + formate + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| lanosterol + [reduced NADPH-hemoprotein reductase] + O2 | natural substrate | Saccharomyces cerevisiae | 4,4-dimethyl-5alpha-cholesta-8,14,24-trien-3beta-ol + formate + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| lanosterol + [reduced NADPH-hemoprotein reductase] + O2 | lanosta-8,24-dien-3beta-ol | Rattus norvegicus | 4,4-dimethyl-5alpha-cholesta-8,14,24-trien-3beta-ol + formate + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| lanosterol + [reduced NADPH-hemoprotein reductase] + O2 | lanosta-8,24-dien-3beta-ol | Saccharomyces cerevisiae | 4,4-dimethyl-5alpha-cholesta-8,14,24-trien-3beta-ol + formate + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| lanosterol + [reduced NADPH-hemoprotein reductase] + O2 | 4,4,14alpha-trimethyl-5alpha-cholesta-8,24-dien-3beta-ol | Rattus norvegicus | 4,4-dimethyl-5alpha-cholesta-8,14,24-trien-3beta-ol + formate + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| lanosterol + [reduced NADPH-hemoprotein reductase] + O2 | 4,4,14alpha-trimethyl-5alpha-cholesta-8,24-dien-3beta-ol | Saccharomyces cerevisiae | 4,4-dimethyl-5alpha-cholesta-8,14,24-trien-3beta-ol + formate + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| additional information | substrate specificity | Rattus norvegicus | ? | - |
? | |
| additional information | substrate specificity | Saccharomyces cerevisiae | ? | - |
? | |
| additional information | removal of 14alpha-methyl group, C32, from 14alpha-methylated precursor sterols is an essential step of sterol biosynthesis in eukaryotes | Rattus norvegicus | ? | - |
? | |
| additional information | removal of 14alpha-methyl group, C32, from 14alpha-methylated precursor sterols is an essential step of sterol biosynthesis in eukaryotes | Saccharomyces cerevisiae | ? | - |
? | |
| additional information | enzyme of cholesterol biosynthesis | Rattus norvegicus | ? | - |
? | |
| additional information | enzyme of ergosterol biosynthesis | Saccharomyces cerevisiae | ? | - |
? | |
| additional information | enzyme of sterol biosynthetic pathway | Rattus norvegicus | ? | - |
? | |
| additional information | enzyme of sterol biosynthetic pathway | Saccharomyces cerevisiae | ? | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Saccharomyces cerevisiae |
| 30 | - |
aerobic conditions | Saccharomyces cerevisiae |
| 37 | - |
assay at | Rattus norvegicus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| cytochrome P450 | - |
Rattus norvegicus | |
| cytochrome P450 | - |
Saccharomyces cerevisiae | |
| heme | heme-thiolate enzyme | Rattus norvegicus | |
| heme | heme-thiolate enzyme | Saccharomyces cerevisiae | |
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