Literature summary for 1.14.14.15 extracted from

Chang, C.; Lohman, J.; Cao, H.; Tan, K.; Rudolf, J.; Ma, M.; Xu, W.; Bingman, C.; Yennamalli, R.; Bigelow, L.; Babnigg, G.; Yan, X.; Joachimiak, A.; Phillips, G.; Shen, B.
Crystal structures of SgcE6 and SgcC, the two-component monooxygenase that catalyzes hydroxylation of a carrier protein-tethered substrate during the biosynthesis of the enediyne antitumor antibiotic C-1027 in Streptomyces globisporus (2016), Biochemistry, 55, 5142-5154 .

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Crystallization (Commentary)

Crystallization (Comment)	Organism
structures of both flavin reductase subunit SgcE6, and chlorophenol-4-monooxygenase subunit SgcC, t 1.66 and 2.63 A resolution, respectively. A flexible loop near the active site of SgcE6 plays a role in FAD binding	Streptomyces globisporus

Organism

Organism	UniProt	Comment	Textmining
Streptomyces globisporus	Q8GME2 and Q8GMG6	Q8GME2 i.e. flavin reductase subunit SgcE6, Q8GMG6 i.e. chlorophenol-4-monooxygenase subunit SgcC	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
additional information	subunit SgcE6 is responsible for the production of FADH2. FADH2 diffuses to SgcC, where Arg119 activates an O2 molecule to form a C-4a-hydroperoxyflavin intermediate. SgcC2 interacts with SgcC and guides the (S)-3-chloro-beta-tyrosyl moiety into the active site. It is activated by conserved general base His161, which results in electrophilic attack of the hydroxy group of the C-4a-hydroperoxyflavin intermediate, introducing a hydroxy group at the C-5 position of the (S)-3-chloro-beta-tyrosyl moiety. The hydroxyflavin (FADHO-) abstracts a proton from the C-5 atom of the intermediate, resulting in the formation of the rearomatizated (S)-3-chloro-5-hydroxy-beta-tyrosyl-S-SgcC2, which then dissociates from SgcC. Finally, the hydroxyflavin (FADHOH) undergoes dehydration to form FAD, which is released and recycled by SgcE6	Streptomyces globisporus	?	-	?

Synonyms

Synonyms	Comment	Organism
SgcE6	-	Streptomyces globisporus

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Release 2023.1 (January 2023)