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Literature summary for 1.14.14.149 extracted from

  • Greenhagen, B.T.; Griggs, P.; Takahashi, S.; Ralston, L.; Chappell, J.
    Probing sesquiterpene hydroxylase activities in a coupled assay with terpene synthases (2003), Arch. Biochem. Biophys., 409, 385-394.
    View publication on PubMed
Search for more literature for 1.14.14.149

Cloned(Commentary)

Cloned (Comment) Organism
expressed in a WAT11 line of yeast Nicotiana tabacum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1-deoxycapsidiol + [reduced NADPH-hemoprotein reductase] + O2 Nicotiana tabacum
-
 capsidiol + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
3-deoxycapsidiol + [reduced NADPH-hemoprotein reductase] + O2 Nicotiana tabacum
-
 capsidiol + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
5-epiaristolochene + 2 [reduced NADPH-hemoprotein reductase] + 2 O2 Nicotiana tabacum
-
 capsidiol + 2 [oxidized NADPH-hemoprotein reductase] + 2 H2O
-
 ?
5-epiaristolochene + [reduced NADPH-hemoprotein reductase] + O2 Nicotiana tabacum 3-deoxycapsidiol is a reaction product when EAH is incubated with high concentrations (above 0.4 mM) of the 5-epiaristolochene substrate 3-deoxycapsidiol + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
5-epiaristolochene + [reduced NADPH-hemoprotein reductase] + O2 Nicotiana tabacum
-
 1-deoxycapsidiol + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
premnaspirodiene + [reduced NADPH-hemoprotein reductase] + O2 Nicotiana tabacum
-
 solavetivone + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Nicotiana tabacum Q94FM7
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-deoxycapsidiol + [reduced NADPH-hemoprotein reductase] + O2
-
 Nicotiana tabacum capsidiol + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
3-deoxycapsidiol + [reduced NADPH-hemoprotein reductase] + O2
-
 Nicotiana tabacum capsidiol + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
5-epiaristolochene + 2 [reduced NADPH-hemoprotein reductase] + 2 O2
-
 Nicotiana tabacum capsidiol + 2 [oxidized NADPH-hemoprotein reductase] + 2 H2O
-
 ?
5-epiaristolochene + 2 [reduced NADPH-hemoprotein reductase] + 2 O2 EAH mediates regio- and stereospecific hydroxylations at the C1 and C3 positions of 5-epi-aristolochene Nicotiana tabacum capsidiol + 2 [oxidized NADPH-hemoprotein reductase] + 2 H2O
-
 ?
5-epiaristolochene + [reduced NADPH-hemoprotein reductase] + O2
-
 Nicotiana tabacum 3-deoxycapsidiol + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
5-epiaristolochene + [reduced NADPH-hemoprotein reductase] + O2 3-deoxycapsidiol is a reaction product when EAH is incubated with high concentrations (above 0.4 mM) of the 5-epiaristolochene substrate Nicotiana tabacum 3-deoxycapsidiol + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
5-epiaristolochene + [reduced NADPH-hemoprotein reductase] + O2
-
 Nicotiana tabacum 1-deoxycapsidiol + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
premnaspirodiene + [reduced NADPH-hemoprotein reductase] + O2
-
 Nicotiana tabacum solavetivone + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?

Synonyms

Synonyms Comment Organism
5-epi-aristolochene dihydroxylase
-
 Nicotiana tabacum
EAH
-
 Nicotiana tabacum

Cofactor

Cofactor Comment Organism Structure
heme
-
 Nicotiana tabacum