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Literature summary for 1.14.14.14 extracted from

  • Di Nardo, G.; Breitner, M.; Bandino, A.; Ghosh, D.; Jennings, G.K.; Hackett, J.C.; Gilardi, G.
    Evidence for an elevated aspartate pK(a) in the active site of human aromatase (2015), J. Biol. Chem., 290, 1186-1196 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.14.14.14

Protein Variants

Protein Variants Comment Organism
D309N mutant retains its ability to bind to androstenedione across the entire pH range studied Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
anastrozole azole inhibitor, binding is independent of pH Homo sapiens
exemestane steroidal inhibitor, binding is dependent of pH Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 androst-4-ene-3,17-dione the Km values increase 1.5fold from pH 6.5 to 7.4similar to the Kd values, temperature not specified in the publication Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens P11511
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
androst-4-ene-3,17-dione + 3 O2 + 3 reduced flavoproteins
-
 Homo sapiens estrone + formate + 4 H2O + 3 oxidized flavoproteins
-
 ?

Synonyms

Synonyms Comment Organism
CYP19A1
-
 Homo sapiens

pH Range

pH Minimum pH Maximum Comment Organism
additional information
-
 androstenedione binding is pH-dependent. The apparent pKa for Asp309 is 8.2, and the residue is protonated at physiological pH. A D309N mutant binds androstenedione across the entire pH range studied Homo sapiens