Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | NADPH-cytochrome P-450 reductase, EC 1.6.2.4, is required for maximal activity | Oryctolagus cuniculus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
50000 | - |
x * 50000, SDS-PAGE | Oryctolagus cuniculus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
7alpha-hydroxycholest-4-en-3-one + NADPH + H+ + O2 | Oryctolagus cuniculus | microsomal enzyme activity was markedly elevated by starvation or streptozotocin administration to the animals | 7alpha,12alpha-dihydroxycholest-4-en-3-one + NADP+ + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Oryctolagus cuniculus | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
additional information | immunoblotting experiment show no correlation between the enzyme activity and the amount of protein, suggesting that post-translational modification may occur | Oryctolagus cuniculus |
Purification (Comment) | Organism |
---|---|
- |
Oryctolagus cuniculus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.484 | - |
- |
Oryctolagus cuniculus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
7alpha-hydroxycholest-4-en-3-one + NADPH + H+ + O2 | - |
Oryctolagus cuniculus | 7alpha,12alpha-dihydroxycholest-4-en-3-one + NADP+ + H2O | - |
? | |
7alpha-hydroxycholest-4-en-3-one + NADPH + H+ + O2 | microsomal enzyme activity was markedly elevated by starvation or streptozotocin administration to the animals | Oryctolagus cuniculus | 7alpha,12alpha-dihydroxycholest-4-en-3-one + NADP+ + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 50000, SDS-PAGE | Oryctolagus cuniculus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
cytochrome b5 | omission of cytochrome b5 resulted in 40% loss of activity | Oryctolagus cuniculus | |
cytochrome P450 | omission results in complete loss of activity | Oryctolagus cuniculus | |
NADPH | - |
Oryctolagus cuniculus |