Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.14.14.139 extracted from

  • Ishida, H.; Noshiro, M.; Okuda, K.; Coon, M.J.
    Purification and characterization of 7alpha-hydroxy-4-cholesten-3-one 12alpha-hydroxylase (1992), J. Biol. Chem., 267, 21319-21323.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
additional information NADPH-cytochrome P-450 reductase, EC 1.6.2.4, is required for maximal activity Oryctolagus cuniculus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
50000
-
x * 50000, SDS-PAGE Oryctolagus cuniculus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
7alpha-hydroxycholest-4-en-3-one + NADPH + H+ + O2 Oryctolagus cuniculus microsomal enzyme activity was markedly elevated by starvation or streptozotocin administration to the animals 7alpha,12alpha-dihydroxycholest-4-en-3-one + NADP+ + H2O
-
?

Organism

Organism UniProt Comment Textmining
Oryctolagus cuniculus
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
additional information immunoblotting experiment show no correlation between the enzyme activity and the amount of protein, suggesting that post-translational modification may occur Oryctolagus cuniculus

Purification (Commentary)

Purification (Comment) Organism
-
Oryctolagus cuniculus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.484
-
-
Oryctolagus cuniculus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
7alpha-hydroxycholest-4-en-3-one + NADPH + H+ + O2
-
Oryctolagus cuniculus 7alpha,12alpha-dihydroxycholest-4-en-3-one + NADP+ + H2O
-
?
7alpha-hydroxycholest-4-en-3-one + NADPH + H+ + O2 microsomal enzyme activity was markedly elevated by starvation or streptozotocin administration to the animals Oryctolagus cuniculus 7alpha,12alpha-dihydroxycholest-4-en-3-one + NADP+ + H2O
-
?

Subunits

Subunits Comment Organism
? x * 50000, SDS-PAGE Oryctolagus cuniculus

Cofactor

Cofactor Comment Organism Structure
cytochrome b5 omission of cytochrome b5 resulted in 40% loss of activity Oryctolagus cuniculus
cytochrome P450 omission results in complete loss of activity Oryctolagus cuniculus
NADPH
-
Oryctolagus cuniculus