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Literature summary for 1.14.14.137 extracted from

  • Okamoto, M.; Tanaka, Y.; Abrams, S.R.; Kamiya, Y.; Seki, M.; Nambara, E.
    High humidity induces abscisic acid 8-hydroxylase in stomata and vasculature to regulate local and systemic abscisic acid responses in Arabidopsis (2009), Plant Physiol., 149, 825-834.
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
additional information high humidity reduces the abscisic acid levels by the activation of CYP707A1 and CYP707A3 Arabidopsis thaliana

Cloned(Commentary)

Cloned (Comment) Organism
promoter fragment of the translational start of each CYP707A amplified and cloned into pENTR/D-TOPO vector, cloned into the binary vector, pGWB3, with a recombination cassette for the expression of GUS-fused protein. Resulting plasmids electroporated into Agrobacterium strain GV3101, which is used to transform wild-type Arabidopsis accession Columbia plants Arabidopsis thaliana

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana
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Source Tissue

Source Tissue Comment Organism Textmining
guard cell major site of CYP707A1 and CYP707A3 expression Arabidopsis thaliana
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shoot CYP707A3 plays a major role in regulating abscisic acid levels, whereas CYP707A1 plays a minor role in regulating abscisic acid levels in shoots Arabidopsis thaliana
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stoma
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Arabidopsis thaliana
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vascular tissue major site of CYP707A1 and CYP707A3 expression Arabidopsis thaliana
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(S)-abscisate + [reduced NADPH-hemoprotein reductase] + O2
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Arabidopsis thaliana 8'-hydroxyabscisate + [oxidized NADPH-hemoprotein reductase] + H2O
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?

Synonyms

Synonyms Comment Organism
abscisic acid 8'-hydroxylase
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Arabidopsis thaliana
CYP707A1
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Arabidopsis thaliana
CYP707A3
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Arabidopsis thaliana

Expression

Organism Comment Expression
Arabidopsis thaliana when wild-type plants are transferred to high-humidity conditions (relative humidity 90%), CYP707A1 and CYP707A3 transcript levels increase primarily in guard cells and vascular tissues, respectively up

General Information

General Information Comment Organism
physiological function CYP707A1 and CYP707A3 are involved in stomatal opening in response to high humidity. Cyp707a1 and cyp707a3 mutants display lower stomatal conductance under turgid conditions (relative humidity 60%) than the wild-type. Cyp707a3 mutant exhibits high abscisic acid levels even after transferring to high-humidity conditions, whereas, under similar conditions, the cyp707a1 mutant exhibits low abscisic acid levels comparable to the wild-type. Stomatal closure of the cyp707a1 mutant, but not cyp707a3 mutant, is abscisic acid hypersensitive when epidermal peel ias treated with exogenous abscisic acid. CYP707A3 reduces the amount of mobile abscisic acid in vascular tissues in response to high humidity, whereas CYP707A1 inactivates local abscisic acid pools inside the guard cells Arabidopsis thaliana