Inhibitors | Comment | Organism | Structure |
---|---|---|---|
(+-)-3'-methyl-abscisate | competitive, 64% inhibition at 0.05 mM | Arabidopsis thaliana | |
(+-)-abscisic acid-3'-thio-n-butyl thiol | competitive, 51% inhibition at 0.05 mM | Arabidopsis thaliana | |
1'-deoxy-(+)-S-abscisate | competitive, 73% inhibition at 0.05 mM | Arabidopsis thaliana | |
1'-deoxy-1'-fluoro-(+)-S-abscisate | competitive, 100% inhibition at 0.05 mM | Arabidopsis thaliana | |
2'alpha,3'alpha-dihydro-2'alpha,3'alpha-epoxy-(+)-S-abscisate | competitive, 56% inhibition at 0.05 mM | Arabidopsis thaliana | |
3'-azido-(+)-S-abscisate | competitive, 38% inhibition at 0.05 mM | Arabidopsis thaliana | |
3'-bromo-(+)-S-abscisate | competitive, 65% inhibition at 0.05 mM | Arabidopsis thaliana | |
3'-chloro-(+)-S-abscisate | competitive, 70% inhibition at 0.05 mM | Arabidopsis thaliana | |
3'-fluoro-(+)-S-abscisate | competitive, 84% inhibition at 0.05 mM | Arabidopsis thaliana | |
3'-iodo-(+)-S-abscisate | competitive, 54% inhibition at 0.05 mM | Arabidopsis thaliana | |
5'alpha,8'-cyclo-(+)-S-abscisate | competitive, 28% inhibition at 0.05 mM | Arabidopsis thaliana | |
6-nor-(+)-S-abscisate | competitive, 88% inhibition at 0.05 mM | Arabidopsis thaliana | |
7'-methyl-(+)-S-abscisate | competitive, 83% inhibition at 0.05 mM | Arabidopsis thaliana | |
7'-nor-(+)-S-abscisate | competitive, 28% inhibition at 0.05 mM | Arabidopsis thaliana | |
8',8',8'-trifluoro-(+)-S-abscisate | competitive, 38% inhibition at 0.05 mM | Arabidopsis thaliana | |
8',8'-difluoro-(+)-S-abscisate | competitive, 83% inhibition at 0.05 mM | Arabidopsis thaliana | |
8'-fluoro-(+)-S-abscisate | competitive, 83% inhibition at 0.05 mM | Arabidopsis thaliana | |
8'-methyl-(+)-S-abscisate | competitive, 35% inhibition at 0.05 mM | Arabidopsis thaliana | |
8'-methylene-(+)-S-abscisate | competitive, 33% inhibition at 0.05 mM | Arabidopsis thaliana | |
9',9',9'-trifluoro-(+)-S-abscisate | competitive, 55% inhibition at 0.05 mM | Arabidopsis thaliana | |
9',9'-difluoro-(+)-S-abscisate | competitive, 76% inhibition at 0.05 mM | Arabidopsis thaliana | |
9'-fluoro-(+)-S-abscisate | competitive, 83% inhibition at 0.05 mM | Arabidopsis thaliana | |
9'-methyl-(+)-S-abscisate | competitive, 26% inhibition at 0.05 mM | Arabidopsis thaliana | |
abscisic aldehyde | competitive, 31% inhibition at 0.05 mM | Arabidopsis thaliana |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(+)-S-abscisate + [reduced NADPH-hemoprotein reductase] + O2 | - |
Arabidopsis thaliana | 8'-hydroxyabscisate + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
(+-)-3'-methyl-abscisate + [reduced NADPH-hemoprotein reductase] + O2 | 32% of the activity with (+)-S-abscisate | Arabidopsis thaliana | ? + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
(+-)-abscisic acid-3'-thio-n-butyl thiol + [reduced NADPH-hemoprotein reductase] + O2 | 2% of the activity with (+)-S-abscisate | Arabidopsis thaliana | ? + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
1'-deoxy-(+)-S-abscisate + [reduced NADPH-hemoprotein reductase] + O2 | 99% of the activity with (+)-S-abscisate | Arabidopsis thaliana | ? + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
1'-deoxy-1'-fluoro-(+)-S-abscisate + [reduced NADPH-hemoprotein reductase] + O2 | 94% of the activity with (+)-S-abscisate | Arabidopsis thaliana | ? + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
2'alpha,3'alpha-dihydro-2'alpha,3'alpha-epoxy-(+)-S-abscisate + [reduced NADPH-hemoprotein reductase] + O2 | 19% of the activity with (+)-S-abscisate | Arabidopsis thaliana | ? + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
3'-bromo-(+)-S-abscisate + [reduced NADPH-hemoprotein reductase] + O2 | 5% of the activity with (+)-S-abscisate | Arabidopsis thaliana | ? + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
3'-chloro-(+)-S-abscisate + [reduced NADPH-hemoprotein reductase] + O2 | 19% of the activity with (+)-S-abscisate | Arabidopsis thaliana | ? + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
3'-fluoro-(+)-S-abscisate + [reduced NADPH-hemoprotein reductase] + O2 | 68% of the activity with (+)-S-abscisate | Arabidopsis thaliana | ? + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
6-nor-(+)-S-abscisate + [reduced NADPH-hemoprotein reductase] + O2 | 60% of the activity with (+)-S-abscisate | Arabidopsis thaliana | ? + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
7'-methyl-(+)-S-abscisate + [reduced NADPH-hemoprotein reductase] + O2 | 15% of the activity with (+)-S-abscisate | Arabidopsis thaliana | ? + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
7'-nor-(+)-S-abscisate + [reduced NADPH-hemoprotein reductase] + O2 | 15% of the activity with (+)-S-abscisate | Arabidopsis thaliana | ? + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
8'-fluoro-(+)-S-abscisate + [reduced NADPH-hemoprotein reductase] + O2 | 11% of the activity with (+)-S-abscisate | Arabidopsis thaliana | ? + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
8'-methylene-(+)-S-abscisate + [reduced NADPH-hemoprotein reductase] + O2 | 4% of the activity with (+)-S-abscisate | Arabidopsis thaliana | ? + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
9',9'-difluoro-(+)-S-abscisate + [reduced NADPH-hemoprotein reductase] + O2 | 3% of the activity with (+)-S-abscisate | Arabidopsis thaliana | 9',9'-difluoro-8'-hydroxy-(+)-S-abscisate + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
9'-fluoro-(+)-S-abscisate + [reduced NADPH-hemoprotein reductase] + O2 | 33% of the activity with (+)-S-abscisate | Arabidopsis thaliana | ? + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
9'-methyl-(+)-S-abscisate + [reduced NADPH-hemoprotein reductase] + O2 | 3% of the activity with (+)-S-abscisate | Arabidopsis thaliana | ? + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
additional information | substrate recognition strictly requires the 6'-methyl groups | Arabidopsis thaliana | ? | - |
? |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00016 | - |
6-nor-(+)-S-abscisate | pH 7.25, 30°C | Arabidopsis thaliana | |
0.00017 | - |
8',8'-difluoro-(+)-S-abscisate | pH 7.25, 30°C | Arabidopsis thaliana | |
0.00025 | - |
9',9'-difluoro-(+)-S-abscisate | pH 7.25, 30°C | Arabidopsis thaliana | |
0.00027 | - |
8'-fluoro-(+)-S-abscisate | pH 7.25, 30°C | Arabidopsis thaliana | |
0.00071 | - |
8',8',8'-trifluoro-(+)-S-abscisate | pH 7.25, 30°C | Arabidopsis thaliana | |
0.00078 | - |
9'-fluoro-(+)-S-abscisate | pH 7.25, 30°C | Arabidopsis thaliana | |
0.00094 | - |
8'-methyl-(+)-S-abscisate | pH 7.25, 30°C | Arabidopsis thaliana | |
0.00106 | - |
9',9',9'-trifluoro-(+)-S-abscisate | pH 7.25, 30°C | Arabidopsis thaliana | |
0.00429 | - |
9'-methyl-(+)-S-abscisate | pH 7.25, 30°C | Arabidopsis thaliana | |
0.00543 | - |
8'-methylene-(+)-S-abscisate | pH 7.25, 30°C | Arabidopsis thaliana |