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Literature summary for 1.14.14.137 extracted from

  • Ueno, K.; Araki, Y.; Hirai, N.; Saito, S.; Mizutani, M.; Sakata, K.; Todoroki, Y.
    Differences between the structural requirements for ABA 8'-hydroxylase inhibition and for ABA activity (2005), Bioorg. Med. Chem., 13, 3359-3370.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
(+-)-3'-methyl-abscisate competitive, 64% inhibition at 0.05 mM Arabidopsis thaliana
(+-)-abscisic acid-3'-thio-n-butyl thiol competitive, 51% inhibition at 0.05 mM Arabidopsis thaliana
1'-deoxy-(+)-S-abscisate competitive, 73% inhibition at 0.05 mM Arabidopsis thaliana
1'-deoxy-1'-fluoro-(+)-S-abscisate competitive, 100% inhibition at 0.05 mM Arabidopsis thaliana
2'alpha,3'alpha-dihydro-2'alpha,3'alpha-epoxy-(+)-S-abscisate competitive, 56% inhibition at 0.05 mM Arabidopsis thaliana
3'-azido-(+)-S-abscisate competitive, 38% inhibition at 0.05 mM Arabidopsis thaliana
3'-bromo-(+)-S-abscisate competitive, 65% inhibition at 0.05 mM Arabidopsis thaliana
3'-chloro-(+)-S-abscisate competitive, 70% inhibition at 0.05 mM Arabidopsis thaliana
3'-fluoro-(+)-S-abscisate competitive, 84% inhibition at 0.05 mM Arabidopsis thaliana
3'-iodo-(+)-S-abscisate competitive, 54% inhibition at 0.05 mM Arabidopsis thaliana
5'alpha,8'-cyclo-(+)-S-abscisate competitive, 28% inhibition at 0.05 mM Arabidopsis thaliana
6-nor-(+)-S-abscisate competitive, 88% inhibition at 0.05 mM Arabidopsis thaliana
7'-methyl-(+)-S-abscisate competitive, 83% inhibition at 0.05 mM Arabidopsis thaliana
7'-nor-(+)-S-abscisate competitive, 28% inhibition at 0.05 mM Arabidopsis thaliana
8',8',8'-trifluoro-(+)-S-abscisate competitive, 38% inhibition at 0.05 mM Arabidopsis thaliana
8',8'-difluoro-(+)-S-abscisate competitive, 83% inhibition at 0.05 mM Arabidopsis thaliana
8'-fluoro-(+)-S-abscisate competitive, 83% inhibition at 0.05 mM Arabidopsis thaliana
8'-methyl-(+)-S-abscisate competitive, 35% inhibition at 0.05 mM Arabidopsis thaliana
8'-methylene-(+)-S-abscisate competitive, 33% inhibition at 0.05 mM Arabidopsis thaliana
9',9',9'-trifluoro-(+)-S-abscisate competitive, 55% inhibition at 0.05 mM Arabidopsis thaliana
9',9'-difluoro-(+)-S-abscisate competitive, 76% inhibition at 0.05 mM Arabidopsis thaliana
9'-fluoro-(+)-S-abscisate competitive, 83% inhibition at 0.05 mM Arabidopsis thaliana
9'-methyl-(+)-S-abscisate competitive, 26% inhibition at 0.05 mM Arabidopsis thaliana
abscisic aldehyde competitive, 31% inhibition at 0.05 mM Arabidopsis thaliana

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(+)-S-abscisate + [reduced NADPH-hemoprotein reductase] + O2
-
Arabidopsis thaliana 8'-hydroxyabscisate + [oxidized NADPH-hemoprotein reductase] + H2O
-
?
(+-)-3'-methyl-abscisate + [reduced NADPH-hemoprotein reductase] + O2 32% of the activity with (+)-S-abscisate Arabidopsis thaliana ? + [oxidized NADPH-hemoprotein reductase] + H2O
-
?
(+-)-abscisic acid-3'-thio-n-butyl thiol + [reduced NADPH-hemoprotein reductase] + O2 2% of the activity with (+)-S-abscisate Arabidopsis thaliana ? + [oxidized NADPH-hemoprotein reductase] + H2O
-
?
1'-deoxy-(+)-S-abscisate + [reduced NADPH-hemoprotein reductase] + O2 99% of the activity with (+)-S-abscisate Arabidopsis thaliana ? + [oxidized NADPH-hemoprotein reductase] + H2O
-
?
1'-deoxy-1'-fluoro-(+)-S-abscisate + [reduced NADPH-hemoprotein reductase] + O2 94% of the activity with (+)-S-abscisate Arabidopsis thaliana ? + [oxidized NADPH-hemoprotein reductase] + H2O
-
?
2'alpha,3'alpha-dihydro-2'alpha,3'alpha-epoxy-(+)-S-abscisate + [reduced NADPH-hemoprotein reductase] + O2 19% of the activity with (+)-S-abscisate Arabidopsis thaliana ? + [oxidized NADPH-hemoprotein reductase] + H2O
-
?
3'-bromo-(+)-S-abscisate + [reduced NADPH-hemoprotein reductase] + O2 5% of the activity with (+)-S-abscisate Arabidopsis thaliana ? + [oxidized NADPH-hemoprotein reductase] + H2O
-
?
3'-chloro-(+)-S-abscisate + [reduced NADPH-hemoprotein reductase] + O2 19% of the activity with (+)-S-abscisate Arabidopsis thaliana ? + [oxidized NADPH-hemoprotein reductase] + H2O
-
?
3'-fluoro-(+)-S-abscisate + [reduced NADPH-hemoprotein reductase] + O2 68% of the activity with (+)-S-abscisate Arabidopsis thaliana ? + [oxidized NADPH-hemoprotein reductase] + H2O
-
?
6-nor-(+)-S-abscisate + [reduced NADPH-hemoprotein reductase] + O2 60% of the activity with (+)-S-abscisate Arabidopsis thaliana ? + [oxidized NADPH-hemoprotein reductase] + H2O
-
?
7'-methyl-(+)-S-abscisate + [reduced NADPH-hemoprotein reductase] + O2 15% of the activity with (+)-S-abscisate Arabidopsis thaliana ? + [oxidized NADPH-hemoprotein reductase] + H2O
-
?
7'-nor-(+)-S-abscisate + [reduced NADPH-hemoprotein reductase] + O2 15% of the activity with (+)-S-abscisate Arabidopsis thaliana ? + [oxidized NADPH-hemoprotein reductase] + H2O
-
?
8'-fluoro-(+)-S-abscisate + [reduced NADPH-hemoprotein reductase] + O2 11% of the activity with (+)-S-abscisate Arabidopsis thaliana ? + [oxidized NADPH-hemoprotein reductase] + H2O
-
?
8'-methylene-(+)-S-abscisate + [reduced NADPH-hemoprotein reductase] + O2 4% of the activity with (+)-S-abscisate Arabidopsis thaliana ? + [oxidized NADPH-hemoprotein reductase] + H2O
-
?
9',9'-difluoro-(+)-S-abscisate + [reduced NADPH-hemoprotein reductase] + O2 3% of the activity with (+)-S-abscisate Arabidopsis thaliana 9',9'-difluoro-8'-hydroxy-(+)-S-abscisate + [oxidized NADPH-hemoprotein reductase] + H2O
-
?
9'-fluoro-(+)-S-abscisate + [reduced NADPH-hemoprotein reductase] + O2 33% of the activity with (+)-S-abscisate Arabidopsis thaliana ? + [oxidized NADPH-hemoprotein reductase] + H2O
-
?
9'-methyl-(+)-S-abscisate + [reduced NADPH-hemoprotein reductase] + O2 3% of the activity with (+)-S-abscisate Arabidopsis thaliana ? + [oxidized NADPH-hemoprotein reductase] + H2O
-
?
additional information substrate recognition strictly requires the 6'-methyl groups Arabidopsis thaliana ?
-
?

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.00016
-
6-nor-(+)-S-abscisate pH 7.25, 30°C Arabidopsis thaliana
0.00017
-
8',8'-difluoro-(+)-S-abscisate pH 7.25, 30°C Arabidopsis thaliana
0.00025
-
9',9'-difluoro-(+)-S-abscisate pH 7.25, 30°C Arabidopsis thaliana
0.00027
-
8'-fluoro-(+)-S-abscisate pH 7.25, 30°C Arabidopsis thaliana
0.00071
-
8',8',8'-trifluoro-(+)-S-abscisate pH 7.25, 30°C Arabidopsis thaliana
0.00078
-
9'-fluoro-(+)-S-abscisate pH 7.25, 30°C Arabidopsis thaliana
0.00094
-
8'-methyl-(+)-S-abscisate pH 7.25, 30°C Arabidopsis thaliana
0.00106
-
9',9',9'-trifluoro-(+)-S-abscisate pH 7.25, 30°C Arabidopsis thaliana
0.00429
-
9'-methyl-(+)-S-abscisate pH 7.25, 30°C Arabidopsis thaliana
0.00543
-
8'-methylene-(+)-S-abscisate pH 7.25, 30°C Arabidopsis thaliana