Inhibitors | Comment | Organism | Structure |
---|---|---|---|
(-)-AHI1 | competitive inhibition | Arabidopsis thaliana | |
(1'R)-(-)-4'-deoxo-abscisic acid | competitive inhibition | Arabidopsis thaliana | |
(1'R)-(-)-6-nor-abscisic acid | competitive inhibition | Arabidopsis thaliana | |
(1'R,2'R)-(-)-2',3'-dihydro-4'-deoxo-abscisic acid | competitive inhibition | Arabidopsis thaliana | |
additional information | inhibitor synthesis, overview. Conformational energy profiles of ligands by computational molecular dynamics simulation, inhibition kinetics, overview. No inhibition by (1'R,2'R)-(-)-2',3'-dihydro-abscisic acid | Arabidopsis thaliana |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
microsome | - |
Arabidopsis thaliana | - |
- |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(1'S)-(+)-abscisate + [reduced NADPH-hemoprotein reductase] + O2 | Arabidopsis thaliana | the enzyme is active with the naturally occuring (1'S)-(+)-enantiomer, but not with the naturally not occuring enantiomer (1'R)-(-)-abscisic acid. The C4'-oxo moiety coupled to the C2'-C3'-double bond in the key functional group for the enzyme to distinguish (1'S)-(+)-abscisic acid from (1'R)-(-)-abscisic acid | 8'-hydroxyabscisate + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(1'S)-(+)-abscisate + [reduced NADPH-hemoprotein reductase] + O2 | the enzyme is active with the naturally occuring (1'S)-(+)-enantiomer, but not with the naturally not occuring enantiomer (1'R)-(-)-abscisic acid. The C4'-oxo moiety coupled to the C2'-C3'-double bond in the key functional group for the enzyme to distinguish (1'S)-(+)-abscisic acid from (1'R)-(-)-abscisic acid | Arabidopsis thaliana | 8'-hydroxyabscisate + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
(2Z,4E)-5-[(1R,6R)-1-hydroxy-2,2,6-trimethylcyclohexyl]penta-2,4-dienoic acid + [reduced NADPH-hemoprotein reductase] + O2 | a structural analogue of abscisic acid lacking the C6 methyl group and the alpha,beta-unsaturated carbonyl in the six-membered ring, synthesis, overview. Both enantiomers of this analogue bind to the enzyme | Arabidopsis thaliana | ? + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
(2Z,4E)-5-[(1S,6S)-1-hydroxy-2,2,6-trimethylcyclohexyl]penta-2,4-dienoic acid + [reduced NADPH-hemoprotein reductase] + O2 | a structural analogue of abscisic acid lacking the C6 methyl group and the alpha,beta-unsaturated carbonyl in the six-membered ring, synthesis, overview. Both enantiomers of this analogue bind to the enzyme | Arabidopsis thaliana | ? + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ABA 8'-hydroxylase | - |
Arabidopsis thaliana |
abscisic acid 8'-hydroxylase | - |
Arabidopsis thaliana |
CYP707A | - |
Arabidopsis thaliana |
CYP707A3 | - |
Arabidopsis thaliana |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Arabidopsis thaliana |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.2 | 7.3 | assay at | Arabidopsis thaliana |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00045 | - |
(1'R,2'R)-(-)-2',3'-dihydro-4'-deoxo-abscisic acid | pH 7.2-7.3, 30°C | Arabidopsis thaliana | |
0.027 | - |
(1'R)-(-)-4'-deoxo-abscisic acid | pH 7.2-7.3, 30°C | Arabidopsis thaliana |
General Information | Comment | Organism |
---|---|---|
metabolism | ABA 8'-hydroxylase is the major and key P450 enzyme in the abscisic acid catabolism | Arabidopsis thaliana |
physiological function | ABA 8'-hydroxylase is the major and key P450 enzyme in the abscisic acid catabolism | Arabidopsis thaliana |