BRENDA - Enzyme Database
show all sequences of 1.14.14.136

Deoxysarpagine hydroxylase--a novel enzyme closing a short side pathway of alkaloid biosynthesis in Rauvolfia

Yu, B.; Ruppert, M.; Stockigt, J.; Bioorg. Med. Chem. 10, 2479-2483 (2002)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
Ancymidole
1.0 mM, 49% inhibition
Rauvolfia serpentina
BAS 110 W
1 mM, complete inhibition
Rauvolfia serpentina
BAS 111 W
1 mM, 84% inhibition
Rauvolfia serpentina
CO
ratio CO:O2 of 9:1, 70% inhibition in the light, 80% inhibition under red light, 77% inhibition under blue light
Rauvolfia serpentina
cytochrome c
0.01 mM, 46% inhibition
Rauvolfia serpentina
ketoconazole
1 mM, 69% inhibition
Rauvolfia serpentina
LAB 1500978
1 mM, 74% inhibition
Rauvolfia serpentina
Metyrapone
1 mM, 83% inhibition
Rauvolfia serpentina
NADP+
competitive inhibition
Rauvolfia serpentina
Tetcyclacis
1 mM, 45% inhibition
Rauvolfia serpentina
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0074
-
10-deoxysarpagine
35°C, pH 8.0
Rauvolfia serpentina
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
membrane
associated
Rauvolfia serpentina
16020
-
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
10-deoxysarpagine + [reduced NADPH-hemoprotein reductase] + O2
Rauvolfia serpentina
most probably the natural substrate
sarpagine + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
?
Organism
Organism
UniProt
Commentary
Textmining
Rauvolfia serpentina
-
-
-
Purification (Commentary)
Purification (Commentary)
Organism
-
Rauvolfia serpentina
Storage Stability
Storage Stability
Organism
-25°C, 20% sucrose, more than 3 months, no loss of activity
Rauvolfia serpentina
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
10-deoxysarpagine + [reduced NADPH-hemoprotein reductase] + O2
-
658260
Rauvolfia serpentina
sarpagine + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
10-deoxysarpagine + [reduced NADPH-hemoprotein reductase] + O2
most probably the natural substrate
658260
Rauvolfia serpentina
sarpagine + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
35
-
-
Rauvolfia serpentina
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
-
Rauvolfia serpentina
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
Ancymidole
1.0 mM, 49% inhibition
Rauvolfia serpentina
BAS 110 W
1 mM, complete inhibition
Rauvolfia serpentina
BAS 111 W
1 mM, 84% inhibition
Rauvolfia serpentina
CO
ratio CO:O2 of 9:1, 70% inhibition in the light, 80% inhibition under red light, 77% inhibition under blue light
Rauvolfia serpentina
cytochrome c
0.01 mM, 46% inhibition
Rauvolfia serpentina
ketoconazole
1 mM, 69% inhibition
Rauvolfia serpentina
LAB 1500978
1 mM, 74% inhibition
Rauvolfia serpentina
Metyrapone
1 mM, 83% inhibition
Rauvolfia serpentina
NADP+
competitive inhibition
Rauvolfia serpentina
Tetcyclacis
1 mM, 45% inhibition
Rauvolfia serpentina
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0074
-
10-deoxysarpagine
35°C, pH 8.0
Rauvolfia serpentina
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
membrane
associated
Rauvolfia serpentina
16020
-
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
10-deoxysarpagine + [reduced NADPH-hemoprotein reductase] + O2
Rauvolfia serpentina
most probably the natural substrate
sarpagine + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
-
Rauvolfia serpentina
Storage Stability (protein specific)
Storage Stability
Organism
-25°C, 20% sucrose, more than 3 months, no loss of activity
Rauvolfia serpentina
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
10-deoxysarpagine + [reduced NADPH-hemoprotein reductase] + O2
-
658260
Rauvolfia serpentina
sarpagine + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
10-deoxysarpagine + [reduced NADPH-hemoprotein reductase] + O2
most probably the natural substrate
658260
Rauvolfia serpentina
sarpagine + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
35
-
-
Rauvolfia serpentina
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
-
Rauvolfia serpentina
Other publictions for EC 1.14.14.136
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
658260
Yu
Deoxysarpagine hydroxylase--a ...
Rauvolfia serpentina
Bioorg. Med. Chem.
10
2479-2483
2002
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10
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1
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1
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4
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1
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10
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1
1
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1
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1
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1
2
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1
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1
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