Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | challenge with either a glucan elicitor from Phytophthora megasperma f.sp. glycinea or with yeast extract causes strong stimulation of cyclase activity with a maximum at about 24 h after elicitor addition | Glycine max | |
NADH | a synergistic effect of NADH, 94% increase in cyclase activity, is observed at low NADPH concentrations of about 0.01 mM | Glycine max |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ancymidol | 0.01 mM: 15% inhibition, 0.1 mM: 85% inhibition | Glycine max | |
BAS 110 | 0.1 mM, 15% inhibition | Glycine max | |
BAS 111 | 0.1 mM, 39% inhibition | Glycine max | |
CO | inhibits in a white light-reversible manner in the presence of oxygen | Glycine max | |
cytochrome c | 0.01 mM: 25% inhibition, 0.025 mM: 64% inhibition, 0.05 mM: 85% inhibition | Glycine max | |
glyceollin | product inhibition, 0.058 mM, 50% inhibition | Glycine max | |
ketoconazol | 0.01 mM: 34% inhibition, 0.1 mM: 100% inhibition | Glycine max | |
LAB 978 | 0.01 mM: 17% inhibition, 0.1 mM: 49% inhibition | Glycine max | |
Metyrapone | 0.01 mM: 15% inhibition, 0.1 mM: 37% inhibition | Glycine max | |
NADP+ | strong inhibition by NADP+ at low NADPH concentrations | Glycine max | |
potassium isothiocyanate | 0.01 mM: 19% inhibition, 0.1 mM: 73% inhibition, 1 mM: 100% inhibition | Glycine max | |
SKF 525 | 0.1 mM: 11% inhibition, 1 mM: 100% inhibition | Glycine max | |
tetcyclasis | 0.01 mM: 9% inhibition, 0.1 mM: 67% inhibition | Glycine max |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.002 | - |
2-(or 4-)dimethylallyl-(6aS,11aS)-3,6a,9-trihydroxypterocarpan | pH 7.5, 20°C, mixture of the two glyceollidin isomers | Glycine max | |
0.03 | - |
perrhenate | pH 7.5, 20°C | Glycine max |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
endoplasmic reticulum | the cyclase is associated with | Glycine max | 5783 | - |
microsome | - |
Glycine max | - |
- |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Glycine max | last committed step in the glyceollin biosynthesis | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Glycine max | - |
cv. Harosoy 63 | - |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
2-dimethylallyl-(6aS,11aS)-3,6a,9-trihydroxypterocarpan + [reduced NADPH-hemoprotein reductase] + O2 = glyceollin II + [oxidized NADPH-hemoprotein reductase] + 2 H2O | a heme-thiolate protein, P-450, glyceollins II and III are formed from 2-dimethylallyl-(6aS,11aS)-3,6a,9-trihydroxypterocarpan whereas glyceollin I is formed from the 4-isomer | Glycine max |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cell suspension culture | elicitor-challenged, unstimulated cell culture does not contain detectable cyclase activity | Glycine max | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Glycine max |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-(or 4-)dimethylallyl-(6aS,11aS)-3,6a,9-trihydroxypterocarpan + [reduced NADPH-hemoprotein reductase] + O2 | enzyme catalyzes an NADPH-dependent and oxygen-dependent cyclization of a mixture of 2- and 4-dimethylallylglycinols to the glyceollin isomers I-III | Glycine max | glyceollin + [oxidized NADPH-hemoprotein reductase] + H2O | glyceollin isomers I-III, glyceollin isomer I is formed from 4-dimethylallylglycinol and glyceollin isomers II and III from 2-dimethylallylglycinol | ? | |
glyceollin II + NADPH + H+ + H2O | - |
Glycine max | 2-dimethylallyl-(6aS,11aS)-3,6a,9-trihydroxypterocarpan + NADP+ + O2 | - |
r | |
additional information | last committed step in the glyceollin biosynthesis | Glycine max | ? | - |
? | |
additional information | not: NADH, enzyme does not catalyze the conversion of rot-2-enoic acid to deguelin in the presence or absence of NADPH | Glycine max | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
dimethylallyl-3,6a,9-trihydroxypterocarpan cyclase | - |
Glycine max |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | - |
assay at | Glycine max |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | 9.5 | very broad pH-optimum | Glycine max |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7 | 9.5 | pH 7: 50% of maximum activity, no activity measurable above pH 9.5, probably because of the instability of the substrate | Glycine max |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
cytochrome b | average content of b-type cytochromes: 0.26 nmol/mg protein | Glycine max | |
cytochrome P-450 | cytochrome P-450-dependent monooxygenase, average content of cytochrome P-450: 0.16 nmol/mg protein | Glycine max | |
O2 | oxygen-dependent | Glycine max |