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Literature summary for 1.14.14.126 extracted from

  • Han, J.Y.; Chun, J.H.; O, S.A.; Park, S.B.; Hwang, H.S.; Lee, H.; Choi, Y.E.
    Transcriptomic analysis of Kalopanax septemlobus and characterization of KsBAS, CYP716A94, and CYP72A397 genes involved in hederagenin saponin biosynthesis (2017), Plant Cell Physiol., 59, 319-330 .
    View publication on PubMed
Search for more literature for 1.14.14.126

Cloned(Commentary)

Cloned (Comment) Organism
gene CYP716A94, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, recombinant expression in Saccharomyces cerevisiae strain WAT21 microsomes, coexpression with Arabidopsis thaliana NADPH-CYP reductase leads to production of oleanolic acid, mass spectrometric analysis Kalopanax septemlobus

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane a transmembrane enzyme Kalopanax septemlobus 16020
-
microsome
-
 Kalopanax septemlobus
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ bound in heme Kalopanax septemlobus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
beta-amyrin + 3 [reduced NADPH-hemoprotein reductase] + 3 O2 Kalopanax septemlobus
-
 oleanolate + [oxidized NADPH-hemoprotein reductase] + 4 H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Kalopanax septemlobus A0A0S2II38
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
beta-amyrin + 3 [reduced NADPH-hemoprotein reductase] + 3 O2
-
 Kalopanax septemlobus oleanolate + [oxidized NADPH-hemoprotein reductase] + 4 H2O
-
 ?

Synonyms

Synonyms Comment Organism
beta-amyrin 28-oxidase
-
 Kalopanax septemlobus
CYP716A94
-
 Kalopanax septemlobus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 in vivo assay at Kalopanax septemlobus

Cofactor

Cofactor Comment Organism Structure
cytochrome P450
-
 Kalopanax septemlobus
heme
-
 Kalopanax septemlobus
NADPH-hemoprotein reductase A flavoprotein containing both FMN and FAD. This enzyme catalyses the transfer of electrons from NADPH, an obligatory two-electron donor, to microsomal P-450 monooxygenases, EC 1.14.14._ Kalopanax septemlobus

General Information

General Information Comment Organism
metabolism the enzyme catalyzes a step in the putative saponin biosynthetic pathway from 2,3-oxidosqualene to hederagenin saponin, overview. Two enzymes (beta-amyrin synthase and beta-amyrin 28-oxidase) are essential for oleanane-type saponin biosynthesis from 2,3-oxidosqualene Kalopanax septemlobus
physiological function CYP716A94 is a beta-amyrin 28-oxidase for the production of oleanolic acid Kalopanax septemlobus