BRENDA - Enzyme Database show
show all sequences of 1.14.14.126

Cytochrome P450 monooxygenase CYP716A141 is a unique beta-amyrin C-16beta oxidase involved in triterpenoid saponin biosynthesis in Platycodon grandiflorus

Tamura, K.; Teranishi, Y.; Ueda, S.; Suzuki, H.; Kawano, N.; Yoshimatsu, K.; Saito, K.; Kawahara, N.; Muranaka, T.; Seki, H.; Plant Cell Physiol. 58, 874-884 (2017)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
gene CYP716A140v2, DNA and amino acid sequence determination and analysis, phylogenetic tree, quantitative real-time PCR enzyme expression analysis, recombinant coexpression of genes CYP716A140v2 and CYP716A141 in Saccharomyces cerevisiae strain 10, that shows increased cochalic acid levels; gene CYP716A141, DNA and amino acid sequence determination and analysis, phylogenetic tree, quantitative real-time PCR enzyme expression analysis, recombinant coexpression of genes CYP716A140v2 and CYP716A141 in Saccharomyces cerevisiae strain 10, that shows increased cochalic acid levels
Platycodon grandiflorus
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
beta-amyrin + [reduced NADPH-hemoprotein reductase] + O2
Platycodon grandiflorus
hydroxylation at C-16beta
maniladiol + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
?
beta-amyrin + [reduced NADPH-hemoprotein reductase] + O2
Platycodon grandiflorus
hydroxylation at C-28
oleanolic acid + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
?
maniladiol + [reduced NADPH-hemoprotein reductase] + O2
Platycodon grandiflorus
hydroxylation at C-28
cochalic acid + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
?
oleanolic acid + [reduced NADPH-hemoprotein reductase] + O2
Platycodon grandiflorus
hydroxylation at C-16beta, reaction of EC 1.14.13.173
cochalic acid + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Platycodon grandiflorus
A0A1I9Q5Z0
-
-
Platycodon grandiflorus
A0A1V1FHP8
-
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
leaf
;
Platycodon grandiflorus
-
additional information
transcriptome analysis in different tissues using RNA sequencing technology, overview; transcriptome analysis in different tissues using RNA sequencing technology, overview
Platycodon grandiflorus
-
petal
;
Platycodon grandiflorus
-
root
the enzyme is highly expressed in roots; the enzyme is highly expressed in roots
Platycodon grandiflorus
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
beta-amyrin + 3 [reduced NADPH-hemoprotein reductase] + 3 O2
overall reaction
746048
Platycodon grandiflorus
oleanolate + 3 [oxidized NADPH-hemoprotein reductase] + 4 H2O
-
-
-
?
beta-amyrin + [reduced NADPH-hemoprotein reductase] + O2
-
746048
Platycodon grandiflorus
erythrodiol + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
beta-amyrin + [reduced NADPH-hemoprotein reductase] + O2
hydroxylation at C-16beta
746048
Platycodon grandiflorus
maniladiol + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
beta-amyrin + [reduced NADPH-hemoprotein reductase] + O2
hydroxylation at C-28
746048
Platycodon grandiflorus
oleanolic acid + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
erythrodiol + [reduced NADPH-hemoprotein reductase] + O2
-
746048
Platycodon grandiflorus
oleanolic aldehyde + [oxidized NADPH-hemoprotein reductase]+ 2 H2O
-
-
-
?
maniladiol + [reduced NADPH-hemoprotein reductase] + O2
-
746048
Platycodon grandiflorus
cochalic acid + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
maniladiol + [reduced NADPH-hemoprotein reductase] + O2
hydroxylation at C-28
746048
Platycodon grandiflorus
cochalic acid + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
additional information
CYP716A141 is capable of catalyzing oxidation at both C-16beta and C-28, metabolites analysis by mass spectrometry
746048
Platycodon grandiflorus
?
-
-
-
-
additional information
hydroxylation of beta-amyrin at C-16beta is the main activity of enzyme CYP716A141, but CYP716A141 is capable of catalyzing oxidation at both C-16beta and C-28, metabolites analysis by mass spectrometry. The enzyme hydroxylates oleanolic acid, beta-amyrin, and maniladiol
746048
Platycodon grandiflorus
?
-
-
-
-
oleanolic acid + [reduced NADPH-hemoprotein reductase] + O2
hydroxylation at C-16beta, reaction of EC 1.14.13.173
746048
Platycodon grandiflorus
cochalic acid + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
oleanolic aldehyde + [reduced NADPH-hemoprotein reductase] + O2
-
746048
Platycodon grandiflorus
oleanolate + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
30
-
in vivo assay at; in vivo assay at
Platycodon grandiflorus
Cofactor
Cofactor
Commentary
Organism
Structure
cytochrome P450
;
Platycodon grandiflorus
NADPH-hemoprotein reductase
A flavoprotein containing both FMN and FAD. This enzyme catalyses the transfer of electrons from NADPH, an obligatory two-electron donor, to microsomal P-450 monooxygenases, EC 1.14.14._; A flavoprotein containing both FMN and FAD. This enzyme catalyses the transfer of electrons from NADPH, an obligatory two-electron donor, to microsomal P-450 monooxygenases, EC 1.14.14._
Platycodon grandiflorus
Cloned(Commentary) (protein specific)
Commentary
Organism
gene CYP716A140v2, DNA and amino acid sequence determination and analysis, phylogenetic tree, quantitative real-time PCR enzyme expression analysis, recombinant coexpression of genes CYP716A140v2 and CYP716A141 in Saccharomyces cerevisiae strain 10, that shows increased cochalic acid levels
Platycodon grandiflorus
gene CYP716A141, DNA and amino acid sequence determination and analysis, phylogenetic tree, quantitative real-time PCR enzyme expression analysis, recombinant coexpression of genes CYP716A140v2 and CYP716A141 in Saccharomyces cerevisiae strain 10, that shows increased cochalic acid levels
Platycodon grandiflorus
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
cytochrome P450
-
Platycodon grandiflorus
NADPH-hemoprotein reductase
A flavoprotein containing both FMN and FAD. This enzyme catalyses the transfer of electrons from NADPH, an obligatory two-electron donor, to microsomal P-450 monooxygenases, EC 1.14.14._
Platycodon grandiflorus
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
beta-amyrin + [reduced NADPH-hemoprotein reductase] + O2
Platycodon grandiflorus
hydroxylation at C-16beta
maniladiol + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
?
beta-amyrin + [reduced NADPH-hemoprotein reductase] + O2
Platycodon grandiflorus
hydroxylation at C-28
oleanolic acid + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
?
maniladiol + [reduced NADPH-hemoprotein reductase] + O2
Platycodon grandiflorus
hydroxylation at C-28
cochalic acid + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
?
oleanolic acid + [reduced NADPH-hemoprotein reductase] + O2
Platycodon grandiflorus
hydroxylation at C-16beta, reaction of EC 1.14.13.173
cochalic acid + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
?
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
leaf
-
Platycodon grandiflorus
-
additional information
transcriptome analysis in different tissues using RNA sequencing technology, overview
Platycodon grandiflorus
-
petal
-
Platycodon grandiflorus
-
root
the enzyme is highly expressed in roots
Platycodon grandiflorus
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
beta-amyrin + 3 [reduced NADPH-hemoprotein reductase] + 3 O2
overall reaction
746048
Platycodon grandiflorus
oleanolate + 3 [oxidized NADPH-hemoprotein reductase] + 4 H2O
-
-
-
?
beta-amyrin + [reduced NADPH-hemoprotein reductase] + O2
-
746048
Platycodon grandiflorus
erythrodiol + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
beta-amyrin + [reduced NADPH-hemoprotein reductase] + O2
hydroxylation at C-16beta
746048
Platycodon grandiflorus
maniladiol + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
beta-amyrin + [reduced NADPH-hemoprotein reductase] + O2
hydroxylation at C-28
746048
Platycodon grandiflorus
oleanolic acid + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
erythrodiol + [reduced NADPH-hemoprotein reductase] + O2
-
746048
Platycodon grandiflorus
oleanolic aldehyde + [oxidized NADPH-hemoprotein reductase]+ 2 H2O
-
-
-
?
maniladiol + [reduced NADPH-hemoprotein reductase] + O2
-
746048
Platycodon grandiflorus
cochalic acid + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
maniladiol + [reduced NADPH-hemoprotein reductase] + O2
hydroxylation at C-28
746048
Platycodon grandiflorus
cochalic acid + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
additional information
CYP716A141 is capable of catalyzing oxidation at both C-16beta and C-28, metabolites analysis by mass spectrometry
746048
Platycodon grandiflorus
?
-
-
-
-
additional information
hydroxylation of beta-amyrin at C-16beta is the main activity of enzyme CYP716A141, but CYP716A141 is capable of catalyzing oxidation at both C-16beta and C-28, metabolites analysis by mass spectrometry. The enzyme hydroxylates oleanolic acid, beta-amyrin, and maniladiol
746048
Platycodon grandiflorus
?
-
-
-
-
oleanolic acid + [reduced NADPH-hemoprotein reductase] + O2
hydroxylation at C-16beta, reaction of EC 1.14.13.173
746048
Platycodon grandiflorus
cochalic acid + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
oleanolic aldehyde + [reduced NADPH-hemoprotein reductase] + O2
-
746048
Platycodon grandiflorus
oleanolate + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
30
-
in vivo assay at
Platycodon grandiflorus
General Information
General Information
Commentary
Organism
evolution
the enzyme belongs to the CYP716A subfamily of cytochrome P450 monooxygenases; the enzyme belongs to the CYP716A subfamily of cytochrome P450 monooxygenases
Platycodon grandiflorus
physiological function
CYP716A140v2, catalyzed a three-step oxidation reaction at C-28 on beta-amyrin to produce oleanolic acid, a reaction performed by CYP716A subfamily P450s in a variety of plant species
Platycodon grandiflorus
General Information (protein specific)
General Information
Commentary
Organism
evolution
the enzyme belongs to the CYP716A subfamily of cytochrome P450 monooxygenases
Platycodon grandiflorus
physiological function
CYP716A140v2, catalyzed a three-step oxidation reaction at C-28 on beta-amyrin to produce oleanolic acid, a reaction performed by CYP716A subfamily P450s in a variety of plant species
Platycodon grandiflorus
Other publictions for EC 1.14.14.126
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
744248
Zhan
Cytochrome P450 CYP716A254 ca ...
Anemone flaccida
Biochem. Biophys. Res. Commun.
495
1271-1277
2018
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2
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745002
Yasumoto
Functional characterization o ...
Solanum lycopersicum
Front. Plant Sci.
8
21
2017
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1
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6
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2
4
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745906
Misra
Two CYP716A subfamily cytochr ...
Ocimum basilicum
New Phytol.
214
706-720
2017
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1
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5
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1
3
3
1
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746004
Jo
beta-Amyrin synthase (EsBAS) ...
Eleutherococcus senticosus
Phytochemistry
135
53-63
2017
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1
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1
1
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4
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7
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1
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3
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1
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1
2
2
1
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746048
Tamura
Cytochrome P450 monooxygenase ...
Platycodon grandiflorus
Plant Cell Physiol.
58
874-884
2017
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1
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4
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5
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4
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11
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1
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2
4
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4
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8
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11
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2
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2
3
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746049
Han
Transcriptomic analysis of Ka ...
Kalopanax septemlobus
Plant Cell Physiol.
59
319-330
2017
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1
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2
1
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1
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7
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1
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2
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746060
Tamura
CYP716A179 functions as a tri ...
Glycyrrhiza uralensis, Glycyrrhiza uralensis 308-19
Plant Cell Rep.
36
437-445
2017
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1
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16
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6
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6
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18
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748772
Andre
Multifunctional oxidosqualene ...
Malus domestica
New Phytol.
211
1279-1294
2016
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732623
Han
The involvement of beta-amyrin ...
Panax ginseng
Plant Cell Physiol.
54
2034-2046
2013
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1
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732622
Fukushima
CYP716A subfamily members are ...
Medicago truncatula, Vitis vinifera
Plant Cell Physiol.
52
2050-2061
2011
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2
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19
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732627
Carelli
Medicago truncatula CYP716A12 ...
Medicago truncatula
Plant Cell
23
3070-3081
2011
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