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Literature summary for 1.14.14.126 extracted from

  • Tamura, K.; Teranishi, Y.; Ueda, S.; Suzuki, H.; Kawano, N.; Yoshimatsu, K.; Saito, K.; Kawahara, N.; Muranaka, T.; Seki, H.
    Cytochrome P450 monooxygenase CYP716A141 is a unique beta-amyrin C-16beta oxidase involved in triterpenoid saponin biosynthesis in Platycodon grandiflorus (2017), Plant Cell Physiol., 58, 874-884 .
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
gene CYP716A140v2, DNA and amino acid sequence determination and analysis, phylogenetic tree, quantitative real-time PCR enzyme expression analysis, recombinant coexpression of genes CYP716A140v2 and CYP716A141 in Saccharomyces cerevisiae strain 10, that shows increased cochalic acid levels Platycodon grandiflorus
gene CYP716A141, DNA and amino acid sequence determination and analysis, phylogenetic tree, quantitative real-time PCR enzyme expression analysis, recombinant coexpression of genes CYP716A140v2 and CYP716A141 in Saccharomyces cerevisiae strain 10, that shows increased cochalic acid levels Platycodon grandiflorus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
beta-amyrin + [reduced NADPH-hemoprotein reductase] + O2 Platycodon grandiflorus hydroxylation at C-16beta maniladiol + [oxidized NADPH-hemoprotein reductase] + H2O
-
?
beta-amyrin + [reduced NADPH-hemoprotein reductase] + O2 Platycodon grandiflorus hydroxylation at C-28 oleanolic acid + [oxidized NADPH-hemoprotein reductase] + H2O
-
?
maniladiol + [reduced NADPH-hemoprotein reductase] + O2 Platycodon grandiflorus hydroxylation at C-28 cochalic acid + [oxidized NADPH-hemoprotein reductase] + H2O
-
?
oleanolic acid + [reduced NADPH-hemoprotein reductase] + O2 Platycodon grandiflorus hydroxylation at C-16beta, reaction of EC 1.14.13.173 cochalic acid + [oxidized NADPH-hemoprotein reductase] + H2O
-
?

Organism

Organism UniProt Comment Textmining
Platycodon grandiflorus A0A1I9Q5Z0
-
-
Platycodon grandiflorus A0A1V1FHP8
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-

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
Platycodon grandiflorus
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additional information transcriptome analysis in different tissues using RNA sequencing technology, overview Platycodon grandiflorus
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petal
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Platycodon grandiflorus
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root the enzyme is highly expressed in roots Platycodon grandiflorus
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
beta-amyrin + 3 [reduced NADPH-hemoprotein reductase] + 3 O2 overall reaction Platycodon grandiflorus oleanolate + 3 [oxidized NADPH-hemoprotein reductase] + 4 H2O
-
?
beta-amyrin + [reduced NADPH-hemoprotein reductase] + O2
-
Platycodon grandiflorus erythrodiol + [oxidized NADPH-hemoprotein reductase] + H2O
-
?
beta-amyrin + [reduced NADPH-hemoprotein reductase] + O2 hydroxylation at C-16beta Platycodon grandiflorus maniladiol + [oxidized NADPH-hemoprotein reductase] + H2O
-
?
beta-amyrin + [reduced NADPH-hemoprotein reductase] + O2 hydroxylation at C-28 Platycodon grandiflorus oleanolic acid + [oxidized NADPH-hemoprotein reductase] + H2O
-
?
erythrodiol + [reduced NADPH-hemoprotein reductase] + O2
-
Platycodon grandiflorus oleanolic aldehyde + [oxidized NADPH-hemoprotein reductase] + 2 H2O
-
?
maniladiol + [reduced NADPH-hemoprotein reductase] + O2
-
Platycodon grandiflorus cochalic acid + [oxidized NADPH-hemoprotein reductase] + H2O
-
?
maniladiol + [reduced NADPH-hemoprotein reductase] + O2 hydroxylation at C-28 Platycodon grandiflorus cochalic acid + [oxidized NADPH-hemoprotein reductase] + H2O
-
?
additional information CYP716A141 is capable of catalyzing oxidation at both C-16beta and C-28, metabolites analysis by mass spectrometry Platycodon grandiflorus ?
-
?
additional information hydroxylation of beta-amyrin at C-16beta is the main activity of enzyme CYP716A141, but CYP716A141 is capable of catalyzing oxidation at both C-16beta and C-28, metabolites analysis by mass spectrometry. The enzyme hydroxylates oleanolic acid, beta-amyrin, and maniladiol Platycodon grandiflorus ?
-
?
oleanolic acid + [reduced NADPH-hemoprotein reductase] + O2 hydroxylation at C-16beta, reaction of EC 1.14.13.173 Platycodon grandiflorus cochalic acid + [oxidized NADPH-hemoprotein reductase] + H2O
-
?
oleanolic aldehyde + [reduced NADPH-hemoprotein reductase] + O2
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Platycodon grandiflorus oleanolate + [oxidized NADPH-hemoprotein reductase] + H2O
-
?

Synonyms

Synonyms Comment Organism
beta-amyrin 28-oxidase
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Platycodon grandiflorus
CYP716A140v2
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Platycodon grandiflorus
CYP716A141
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Platycodon grandiflorus
More cf. EC 1.14.13.173, beta-amyrin C-16beta oxidase Platycodon grandiflorus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
in vivo assay at Platycodon grandiflorus

Cofactor

Cofactor Comment Organism Structure
cytochrome P450
-
Platycodon grandiflorus
NADPH-hemoprotein reductase A flavoprotein containing both FMN and FAD. This enzyme catalyses the transfer of electrons from NADPH, an obligatory two-electron donor, to microsomal P-450 monooxygenases, EC 1.14.14._ Platycodon grandiflorus

General Information

General Information Comment Organism
evolution the enzyme belongs to the CYP716A subfamily of cytochrome P450 monooxygenases Platycodon grandiflorus
physiological function CYP716A140v2, catalyzed a three-step oxidation reaction at C-28 on beta-amyrin to produce oleanolic acid, a reaction performed by CYP716A subfamily P450s in a variety of plant species Platycodon grandiflorus