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Literature summary for 1.14.14.126 extracted from

  • Jo, H.J.; Han, J.Y.; Hwang, H.S.; Choi, Y.E.
    beta-Amyrin synthase (EsBAS) and beta-amyrin 28-oxidase (CYP716A244) in oleanane-type triterpene saponin biosynthesis in Eleutherococcus senticosus (2017), Phytochemistry, 135, 53-63 .
    View publication on PubMed
Search for more literature for 1.14.14.126

Cloned(Commentary)

Cloned (Comment) Organism
CYP716A244, DNA and amino acid sequence determination and analysis, phylogenetic analysis. quantitative RT-PCR enzyme expression analysis, transcriptomics analysis of plant leaves, recombinant expression in Saccharomyces cerevisiae, recombinant expression in trangsgenic Nicotiana tabacum, co-expression with beta-amyrin synthase, EsBAS Eleutherococcus senticosus
gene CYP716A244, phylogenetic tree, quantitative real-time PCR expression analysis, transient recombinant expression in Nicotiana benthamiana using the transformation method via Agrobacterium tumefaciens strain GV3101, and transient recombinant expression in Saccharomyces cerevisiae with coexpression of Arabidopsis thaliana NADPH-CYP reductase Eleutherococcus senticosus

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
 Eleutherococcus senticosus 16020
-

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ bound in heme Eleutherococcus senticosus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
beta-amyrin + 3 [reduced NADPH-hemoprotein reductase] + 3 O2 Eleutherococcus senticosus overall reaction oleanolate + 3 [oxidized NADPH-hemoprotein reductase] + 4 H2O
-
 ?
beta-amyrin + [reduced NADPH-hemoprotein reductase] + O2 Eleutherococcus senticosus
-
 erythrodiol + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
erythrodiol + [reduced NADPH-hemoprotein reductase] + O2 Eleutherococcus senticosus
-
 oleanolate + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
additional information Eleutherococcus senticosus CYP716A244 is a beta-amyrin 28-oxidase that converts beta-amyrin into oleanolic acid via formation of erythrodiol ?
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 ?

Organism

Organism UniProt Comment Textmining
Eleutherococcus senticosus A0A1P8W705
-
-

Source Tissue

Source Tissue Comment Organism Textmining
leaf transcriptome analysis Eleutherococcus senticosus
-
root
-
 Eleutherococcus senticosus
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
beta-amyrin + 3 O2 + 3 NADPH + 3 H+
-
 Eleutherococcus senticosus oleanolate + 3 NADP+ + 4 H2O
-
 ?
beta-amyrin + 3 [reduced NADPH-hemoprotein reductase] + 3 O2 overall reaction Eleutherococcus senticosus oleanolate + 3 [oxidized NADPH-hemoprotein reductase] + 4 H2O
-
 ?
beta-amyrin + O2 + NADPH + H+
-
 Eleutherococcus senticosus erythrodiol + NADP+ + H2O
-
 ?
beta-amyrin + [reduced NADPH-hemoprotein reductase] + O2
-
 Eleutherococcus senticosus erythrodiol + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
erythrodiol + [reduced NADPH-hemoprotein reductase] + O2
-
 Eleutherococcus senticosus oleanolate + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
additional information CYP716A244 is a beta-amyrin 28-oxidase that converts beta-amyrin into oleanolic acid via formation of erythrodiol Eleutherococcus senticosus ?
-
 ?
additional information CYP716A244 is a beta-amyrin 28-oxidase that converts beta-amyrin into oleanolic acid via formation of erythrodiol. The enzyme is a multifunctional oxidase enzymes with beta-amyrin 28-hydroxylase, alpha-amyrin 28-hydroxylase, and lupeol 28-hydroxylase activities Eleutherococcus senticosus ?
-
 ?

Subunits

Subunits Comment Organism
? x * 54010, about, sequence calculation Eleutherococcus senticosus

Synonyms

Synonyms Comment Organism
alpha-amyrin 28-hydroxylase
-
 Eleutherococcus senticosus
beta-amyrin 28-hydroxylase
-
 Eleutherococcus senticosus
beta-amyrin 28-oxidase
-
 Eleutherococcus senticosus
CYP716A244
-
 Eleutherococcus senticosus
lupeol 28-hydroxylase
-
 Eleutherococcus senticosus
multifunctional oxidase
-
 Eleutherococcus senticosus

Cofactor

Cofactor Comment Organism Structure
cytochrome P450
-
 Eleutherococcus senticosus
heme
-
 Eleutherococcus senticosus
NADPH-hemoprotein reductase A flavoprotein containing both FMN and FAD. This enzyme catalyses the transfer of electrons from NADPH, an obligatory two-electron donor, to microsomal P-450 monooxygenases, EC 1.14.14._ Eleutherococcus senticosus

Expression

Organism Comment Expression
Eleutherococcus senticosus methyl jasmonate treatment highly enhances CYP716A244 transcription at 12 h after methyl jasmonate treatment, and this enhancement declines as the culture time proceeds up

General Information

General Information Comment Organism
metabolism oleanane-type triterpenes are the major saponin components in Eleutherococcus senticosus. The two enzymes, beta-amyrin synthase and beta-amyrin 28-oxidase, are essential for oleanane-type saponin biosynthesis from 2,3-oxidosqualene Eleutherococcus senticosus
physiological function CYP716A244 is a beta-amyrin 28-oxidase involved in saponin biosynthesis, beta-amyrin is the oleanane-type triterpene backbone. beta-Amyrin is oxidised at the C-28 position in a sequential three-step oxidation by the single cytochrome P450 enzyme to yield oleanolic acid through erythrodiol Eleutherococcus senticosus