BRENDA - Enzyme Database show
show all sequences of 1.14.14.126

Functional characterization of CYP716 family P450 enzymes in triterpenoid biosynthesis in tomato

Yasumoto, S.; Seki, H.; Shimizu, Y.; Fukushima, E.O.; Muranaka, T.; Front. Plant Sci. 8, 21 (2017)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
gene CYP716A44, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, in beta-amyrin synthase (EC 5.4.99.39) and CYP716A44 co-expressing Saccharomyces cerevisiae strains SY57 and SY66 erythrodiol and oleanolic acid are detected as C-28 oxidation product of beta-amyrin; gene CYP716A46, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, in beta-amyrin synthase (EC 5.4.99.39) and CYP716A46 co-expressing Saccharomyces cerevisiae strains SY57 and SY66, erythrodiol and oleanolic acid are detected as C-28 oxidation product of beta-amyrin
Solanum lycopersicum
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
beta-amyrin + 3 O2 + 3 NADPH + 3 H+
Solanum lycopersicum
-
oleanolate + 3 NADP+ + 4 H2O
-
-
?
beta-amyrin + O2 + NADPH + H+
Solanum lycopersicum
-
erythrodiol + NADP+ + H2O
-
-
?
additional information
Solanum lycopersicum
the enzyme produces erythrodiol and oleanolic acid as C-28 oxidation products of beta-amyrin
?
-
-
-
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Solanum lycopersicum
K4BZH9
cv. Micro-Tom
-
Solanum lycopersicum
K4CEE8
cv. Heinz 1706
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
flower
exclusive expresion of CYP716A46 in flowers; very low expresion level
Solanum lycopersicum
-
additional information
no enzyme expression in leaves; no enzyme expression in leaves, roots, and stems
Solanum lycopersicum
-
root
high enzyme level
Solanum lycopersicum
-
stem
very low expresion level
Solanum lycopersicum
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
beta-amyrin + 3 O2 + 3 NADPH + 3 H+
-
745002
Solanum lycopersicum
oleanolate + 3 NADP+ + 4 H2O
-
-
-
?
beta-amyrin + O2 + NADPH + H+
-
745002
Solanum lycopersicum
erythrodiol + NADP+ + H2O
-
-
-
?
additional information
the enzyme produces erythrodiol and oleanolic acid as C-28 oxidation products of beta-amyrin
745002
Solanum lycopersicum
?
-
-
-
-
Cloned(Commentary) (protein specific)
Commentary
Organism
gene CYP716A44, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, in beta-amyrin synthase (EC 5.4.99.39) and CYP716A44 co-expressing Saccharomyces cerevisiae strains SY57 and SY66 erythrodiol and oleanolic acid are detected as C-28 oxidation product of beta-amyrin
Solanum lycopersicum
gene CYP716A46, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, in beta-amyrin synthase (EC 5.4.99.39) and CYP716A46 co-expressing Saccharomyces cerevisiae strains SY57 and SY66, erythrodiol and oleanolic acid are detected as C-28 oxidation product of beta-amyrin
Solanum lycopersicum
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
beta-amyrin + 3 O2 + 3 NADPH + 3 H+
Solanum lycopersicum
-
oleanolate + 3 NADP+ + 4 H2O
-
-
?
beta-amyrin + O2 + NADPH + H+
Solanum lycopersicum
-
erythrodiol + NADP+ + H2O
-
-
?
additional information
Solanum lycopersicum
the enzyme produces erythrodiol and oleanolic acid as C-28 oxidation products of beta-amyrin
?
-
-
-
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
flower
very low expresion level
Solanum lycopersicum
-
flower
exclusive expresion of CYP716A46 in flowers
Solanum lycopersicum
-
additional information
no enzyme expression in leaves
Solanum lycopersicum
-
additional information
no enzyme expression in leaves, roots, and stems
Solanum lycopersicum
-
root
high enzyme level
Solanum lycopersicum
-
stem
very low expresion level
Solanum lycopersicum
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
beta-amyrin + 3 O2 + 3 NADPH + 3 H+
-
745002
Solanum lycopersicum
oleanolate + 3 NADP+ + 4 H2O
-
-
-
?
beta-amyrin + O2 + NADPH + H+
-
745002
Solanum lycopersicum
erythrodiol + NADP+ + H2O
-
-
-
?
additional information
the enzyme produces erythrodiol and oleanolic acid as C-28 oxidation products of beta-amyrin
745002
Solanum lycopersicum
?
-
-
-
-
General Information
General Information
Commentary
Organism
evolution
the enzyme belongs to the CYP716C subfamily, many CYP716 family P450 enzymes are characterized as multifunctional triterpene C-28 oxidases, which oxidize alpha-amyrin and beta-amyrin to the widely distributed triterpenoids ursolic and oleanolic acids, respectively. Isolation of all six CYP716 family genes from tomato, including CYP716A46, and sequence comparisons; the enzyme belongs to the CYP716C subfamily, many CYP716 family P450 enzymes are characterized as multifunctional triterpene C-28 oxidases, which oxidize alpha-amyrin and beta-amyrin to the widely distributed triterpenoids ursolic and oleanolic acids, respectively. Isolation of all six CYP716 family genes from tomato, including gene CYP716A44, and sequence comparisons
Solanum lycopersicum
metabolism
the enzyme is involved in the triterpenoid biosynthesis in tomato; the enzyme is involved in the triterpenoid biosynthesis in tomato
Solanum lycopersicum
General Information (protein specific)
General Information
Commentary
Organism
evolution
the enzyme belongs to the CYP716C subfamily, many CYP716 family P450 enzymes are characterized as multifunctional triterpene C-28 oxidases, which oxidize alpha-amyrin and beta-amyrin to the widely distributed triterpenoids ursolic and oleanolic acids, respectively. Isolation of all six CYP716 family genes from tomato, including gene CYP716A44, and sequence comparisons
Solanum lycopersicum
evolution
the enzyme belongs to the CYP716C subfamily, many CYP716 family P450 enzymes are characterized as multifunctional triterpene C-28 oxidases, which oxidize alpha-amyrin and beta-amyrin to the widely distributed triterpenoids ursolic and oleanolic acids, respectively. Isolation of all six CYP716 family genes from tomato, including CYP716A46, and sequence comparisons
Solanum lycopersicum
metabolism
the enzyme is involved in the triterpenoid biosynthesis in tomato
Solanum lycopersicum
Other publictions for EC 1.14.14.126
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
744248
Zhan
Cytochrome P450 CYP716A254 ca ...
Anemone flaccida
Biochem. Biophys. Res. Commun.
495
1271-1277
2018
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1
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1
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1
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1
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2
2
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745002
Yasumoto
Functional characterization o ...
Solanum lycopersicum
Front. Plant Sci.
8
21
2017
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1
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6
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2
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6
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6
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6
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2
4
-
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-
745906
Misra
Two CYP716A subfamily cytochr ...
Ocimum basilicum
New Phytol.
214
706-720
2017
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-
1
-
1
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4
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5
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3
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5
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3
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1
3
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1
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4
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3
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5
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1
3
3
1
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746004
Jo
beta-Amyrin synthase (EsBAS) ...
Eleutherococcus senticosus
Phytochemistry
135
53-63
2017
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1
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1
1
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4
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7
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2
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7
1
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3
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1
3
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1
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4
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2
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7
1
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1
2
2
1
-
-
746048
Tamura
Cytochrome P450 monooxygenase ...
Platycodon grandiflorus
Plant Cell Physiol.
58
874-884
2017
-
-
1
-
-
-
-
-
-
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4
-
5
-
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4
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11
-
1
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2
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2
4
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4
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8
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11
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2
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2
3
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746049
Han
Transcriptomic analysis of Ka ...
Kalopanax septemlobus
Plant Cell Physiol.
59
319-330
2017
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1
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2
1
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1
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7
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1
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1
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3
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1
3
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1
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1
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1
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1
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2
2
-
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746060
Tamura
CYP716A179 functions as a tri ...
Glycyrrhiza uralensis, Glycyrrhiza uralensis 308-19
Plant Cell Rep.
36
437-445
2017
-
-
1
-
-
-
-
-
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16
-
6
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6
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18
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2
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1
2
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16
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6
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18
-
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-
748772
Andre
Multifunctional oxidosqualene ...
Malus domestica
New Phytol.
211
1279-1294
2016
-
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1
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-
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5
-
2
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1
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6
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1
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1
1
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5
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1
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6
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1
1
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732623
Han
The involvement of beta-amyrin ...
Panax ginseng
Plant Cell Physiol.
54
2034-2046
2013
-
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1
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-
-
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1
1
-
5
-
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2
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2
1
1
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1
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1
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1
1
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2
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2
1
1
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1
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1
1
1
1
-
-
732622
Fukushima
CYP716A subfamily members are ...
Medicago truncatula, Vitis vinifera
Plant Cell Physiol.
52
2050-2061
2011
-
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2
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7
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6
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4
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2
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19
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3
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7
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4
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19
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732627
Carelli
Medicago truncatula CYP716A12 ...
Medicago truncatula
Plant Cell
23
3070-3081
2011
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1
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1
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1
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5
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5
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2
1
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1
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1
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1
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5
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2
1
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1
1
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