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Literature summary for 1.14.14.126 extracted from

  • Yasumoto, S.; Seki, H.; Shimizu, Y.; Fukushima, E.O.; Muranaka, T.
    Functional characterization of CYP716 family P450 enzymes in triterpenoid biosynthesis in tomato (2017), Front. Plant Sci., 8, 21 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
gene CYP716A44, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, in beta-amyrin synthase (EC 5.4.99.39) and CYP716A44 co-expressing Saccharomyces cerevisiae strains SY57 and SY66 erythrodiol and oleanolic acid are detected as C-28 oxidation product of beta-amyrin Solanum lycopersicum
gene CYP716A46, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, in beta-amyrin synthase (EC 5.4.99.39) and CYP716A46 co-expressing Saccharomyces cerevisiae strains SY57 and SY66, erythrodiol and oleanolic acid are detected as C-28 oxidation product of beta-amyrin Solanum lycopersicum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
beta-amyrin + 3 O2 + 3 NADPH + 3 H+ Solanum lycopersicum
-
oleanolate + 3 NADP+ + 4 H2O
-
?
beta-amyrin + O2 + NADPH + H+ Solanum lycopersicum
-
erythrodiol + NADP+ + H2O
-
?
additional information Solanum lycopersicum the enzyme produces erythrodiol and oleanolic acid as C-28 oxidation products of beta-amyrin ?
-
?

Organism

Organism UniProt Comment Textmining
Solanum lycopersicum A0A3Q7HBJ5 cv. Micro-Tom
-
Solanum lycopersicum K4CEE8 cv. Heinz 1706
-

Source Tissue

Source Tissue Comment Organism Textmining
flower exclusive expresion of CYP716A46 in flowers Solanum lycopersicum
-
flower very low expresion level Solanum lycopersicum
-
additional information no enzyme expression in leaves Solanum lycopersicum
-
additional information no enzyme expression in leaves, roots, and stems Solanum lycopersicum
-
root high enzyme level Solanum lycopersicum
-
stem very low expresion level Solanum lycopersicum
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
beta-amyrin + 3 O2 + 3 NADPH + 3 H+
-
Solanum lycopersicum oleanolate + 3 NADP+ + 4 H2O
-
?
beta-amyrin + O2 + NADPH + H+
-
Solanum lycopersicum erythrodiol + NADP+ + H2O
-
?
additional information the enzyme produces erythrodiol and oleanolic acid as C-28 oxidation products of beta-amyrin Solanum lycopersicum ?
-
?

Synonyms

Synonyms Comment Organism
101251676
-
Solanum lycopersicum
101252702
-
Solanum lycopersicum
CYP716A44
-
Solanum lycopersicum
CYP716A46
-
Solanum lycopersicum
More formerly EC 1.14.13.201 Solanum lycopersicum
multifunctional triterpene C-28 oxidase
-
Solanum lycopersicum

General Information

General Information Comment Organism
evolution the enzyme belongs to the CYP716C subfamily, many CYP716 family P450 enzymes are characterized as multifunctional triterpene C-28 oxidases, which oxidize alpha-amyrin and beta-amyrin to the widely distributed triterpenoids ursolic and oleanolic acids, respectively. Isolation of all six CYP716 family genes from tomato, including CYP716A46, and sequence comparisons Solanum lycopersicum
evolution the enzyme belongs to the CYP716C subfamily, many CYP716 family P450 enzymes are characterized as multifunctional triterpene C-28 oxidases, which oxidize alpha-amyrin and beta-amyrin to the widely distributed triterpenoids ursolic and oleanolic acids, respectively. Isolation of all six CYP716 family genes from tomato, including gene CYP716A44, and sequence comparisons Solanum lycopersicum
metabolism the enzyme is involved in the triterpenoid biosynthesis in tomato Solanum lycopersicum