Cloned (Comment) | Organism |
---|---|
gene CYP716A44, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, in beta-amyrin synthase (EC 5.4.99.39) and CYP716A44 co-expressing Saccharomyces cerevisiae strains SY57 and SY66 erythrodiol and oleanolic acid are detected as C-28 oxidation product of beta-amyrin | Solanum lycopersicum |
gene CYP716A46, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, in beta-amyrin synthase (EC 5.4.99.39) and CYP716A46 co-expressing Saccharomyces cerevisiae strains SY57 and SY66, erythrodiol and oleanolic acid are detected as C-28 oxidation product of beta-amyrin | Solanum lycopersicum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
beta-amyrin + 3 O2 + 3 NADPH + 3 H+ | Solanum lycopersicum | - |
oleanolate + 3 NADP+ + 4 H2O | - |
? | |
beta-amyrin + O2 + NADPH + H+ | Solanum lycopersicum | - |
erythrodiol + NADP+ + H2O | - |
? | |
additional information | Solanum lycopersicum | the enzyme produces erythrodiol and oleanolic acid as C-28 oxidation products of beta-amyrin | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Solanum lycopersicum | A0A3Q7HBJ5 | cv. Micro-Tom | - |
Solanum lycopersicum | K4CEE8 | cv. Heinz 1706 | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
flower | exclusive expresion of CYP716A46 in flowers | Solanum lycopersicum | - |
flower | very low expresion level | Solanum lycopersicum | - |
additional information | no enzyme expression in leaves | Solanum lycopersicum | - |
additional information | no enzyme expression in leaves, roots, and stems | Solanum lycopersicum | - |
root | high enzyme level | Solanum lycopersicum | - |
stem | very low expresion level | Solanum lycopersicum | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
beta-amyrin + 3 O2 + 3 NADPH + 3 H+ | - |
Solanum lycopersicum | oleanolate + 3 NADP+ + 4 H2O | - |
? | |
beta-amyrin + O2 + NADPH + H+ | - |
Solanum lycopersicum | erythrodiol + NADP+ + H2O | - |
? | |
additional information | the enzyme produces erythrodiol and oleanolic acid as C-28 oxidation products of beta-amyrin | Solanum lycopersicum | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
101251676 | - |
Solanum lycopersicum |
101252702 | - |
Solanum lycopersicum |
CYP716A44 | - |
Solanum lycopersicum |
CYP716A46 | - |
Solanum lycopersicum |
More | formerly EC 1.14.13.201 | Solanum lycopersicum |
multifunctional triterpene C-28 oxidase | - |
Solanum lycopersicum |
General Information | Comment | Organism |
---|---|---|
evolution | the enzyme belongs to the CYP716C subfamily, many CYP716 family P450 enzymes are characterized as multifunctional triterpene C-28 oxidases, which oxidize alpha-amyrin and beta-amyrin to the widely distributed triterpenoids ursolic and oleanolic acids, respectively. Isolation of all six CYP716 family genes from tomato, including CYP716A46, and sequence comparisons | Solanum lycopersicum |
evolution | the enzyme belongs to the CYP716C subfamily, many CYP716 family P450 enzymes are characterized as multifunctional triterpene C-28 oxidases, which oxidize alpha-amyrin and beta-amyrin to the widely distributed triterpenoids ursolic and oleanolic acids, respectively. Isolation of all six CYP716 family genes from tomato, including gene CYP716A44, and sequence comparisons | Solanum lycopersicum |
metabolism | the enzyme is involved in the triterpenoid biosynthesis in tomato | Solanum lycopersicum |