Cloned (Comment) | Organism |
---|---|
expression in Saccharomyces cerevisiae | Medicago truncatula |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
microsome | - |
Medicago truncatula | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
57000 | - |
x * 57000, SDS-PAGE | Medicago truncatula |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Medicago truncatula | F8J4R7 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
flower | highest expression of the gene | Medicago truncatula | - |
leaf | significant increase in expression is observed in the reproductive stages, with a maximum expression at flowering | Medicago truncatula | - |
additional information | expression of the gene occurs in all the organs and the developmental stages | Medicago truncatula | - |
root | - |
Medicago truncatula | - |
stem | - |
Medicago truncatula | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
beta-amyrin + [reduced NADPH-hemoprotein reductase] + O2 | overall reaction | Medicago truncatula | oleanolate + [oxidized NADPH-hemoprotein reductase] + H2O | CYP716A12 mainly catalyzes the sequential three-step oxidation at the C-28 position necessary to transform beta-amyrin into oleanolic acid | ? | |
erythrodiol + [reduced NADPH-hemoprotein reductase] + O2 | - |
Medicago truncatula | oleanolic aldehyde + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 57000, SDS-PAGE | Medicago truncatula |
Synonyms | Comment | Organism |
---|---|---|
CYP716A12 | - |
Medicago truncatula |
General Information | Comment | Organism |
---|---|---|
physiological function | enzyme is responsible for an early step in the saponin biosynthetic pathway. Mutants in CYP716A12 are unable to produce hemolytic saponins and only synthetize soyasaponins. CYP716A12 catalyzes the oxidation of beta-amyrin and erythrodiol at the C-28 position, yielding oleanolic acid | Medicago truncatula |