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Literature summary for 1.14.14.123 extracted from

  • Wang, Q.; Hillwig, M.; Okada, K.; Yamazaki, K.; Wu, Y.; Swaminathan, S.; Yamane, H.; Peters, R.
    Characterization of CYP76M5-8 indicates metabolic plasticity within a plant biosynthetic gene cluster (2012), J. Biol. Chem., 287, 6159-6168.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.14.14.123

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli C41 cells Oryza sativa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.002
-
 syn-pimaradiene pH and temperature not specified in the publication Oryza sativa
0.004
-
 ent-cassadiene pH and temperature not specified in the publication Oryza sativa
0.004
-
 ent-pimaradiene pH and temperature not specified in the publication Oryza sativa
0.005
-
 ent-kaurene pH and temperature not specified in the publication Oryza sativa
0.007
-
 ent-sandaracopimaradien-3beta-ol pH and temperature not specified in the publication Oryza sativa
0.015
-
 ent-isokaurene pH and temperature not specified in the publication Oryza sativa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ent-sandaracopimaradien-3beta-ol + [reduced NADPH-hemoprotein reductase] + O2 Oryza sativa
-
 oryzalexin D + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Oryza sativa
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ent-cassadiene + [reduced NADPH-hemoprotein reductase] + O2
-
 Oryza sativa ?
-
 ?
ent-isokaurene + [reduced NADPH-hemoprotein reductase] + O2
-
 Oryza sativa ?
-
 ?
ent-kaurene + [reduced NADPH-hemoprotein reductase] + O2
-
 Oryza sativa ?
-
 ?
ent-pimaradiene + [reduced NADPH-hemoprotein reductase] + O2
-
 Oryza sativa ?
-
 ?
ent-sandaracopimaradien-3beta-ol + [reduced NADPH-hemoprotein reductase] + O2
-
 Oryza sativa oryzalexin D + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
additional information no reaction with syn-pimaradien-19-oic acid, syn-stemodene, syn-stemarene, or syn-labdatriene Oryza sativa ?
-
 ?
syn-pimaradiene + [reduced NADPH-hemoprotein reductase] + O2
-
 Oryza sativa momilactone A + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?

Synonyms

Synonyms Comment Organism
CYP76M8
-
 Oryza sativa

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.006
-
 ent-cassadiene pH and temperature not specified in the publication Oryza sativa
0.006
-
 syn-pimaradiene pH and temperature not specified in the publication Oryza sativa
0.009
-
 ent-kaurene pH and temperature not specified in the publication Oryza sativa
0.009
-
 ent-pimaradiene pH and temperature not specified in the publication Oryza sativa
0.011
-
 ent-sandaracopimaradien-3beta-ol pH and temperature not specified in the publication Oryza sativa
0.015
-
 ent-isokaurene pH and temperature not specified in the publication Oryza sativa

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1
-
 ent-isokaurene pH and temperature not specified in the publication Oryza sativa
1.5
-
 ent-cassadiene pH and temperature not specified in the publication Oryza sativa
1.6
-
 ent-sandaracopimaradien-3beta-ol pH and temperature not specified in the publication Oryza sativa
1.8
-
 ent-kaurene pH and temperature not specified in the publication Oryza sativa
2.3
-
 ent-pimaradiene pH and temperature not specified in the publication Oryza sativa
2.6
-
 syn-pimaradiene pH and temperature not specified in the publication Oryza sativa