BRENDA - Enzyme Database
show all sequences of 1.14.14.123

Characterization of CYP76M5-8 indicates metabolic plasticity within a plant biosynthetic gene cluster

Wang, Q.; Hillwig, M.; Okada, K.; Yamazaki, K.; Wu, Y.; Swaminathan, S.; Yamane, H.; Peters, R.; J. Biol. Chem. 287, 6159-6168 (2012)

Data extracted from this reference:

Cloned(Commentary)
Cloned (Commentary)
Organism
expressed in Escherichia coli C41 cells
Oryza sativa
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.002
-
syn-pimaradiene
pH and temperature not specified in the publication
Oryza sativa
0.004
-
ent-cassadiene
pH and temperature not specified in the publication
Oryza sativa
0.004
-
ent-pimaradiene
pH and temperature not specified in the publication
Oryza sativa
0.005
-
ent-kaurene
pH and temperature not specified in the publication
Oryza sativa
0.007
-
ent-sandaracopimaradien-3beta-ol
pH and temperature not specified in the publication
Oryza sativa
0.015
-
ent-isokaurene
pH and temperature not specified in the publication
Oryza sativa
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
ent-sandaracopimaradien-3beta-ol + [reduced NADPH-hemoprotein reductase] + O2
Oryza sativa
-
oryzalexin D + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
?
Organism
Organism
UniProt
Commentary
Textmining
Oryza sativa
-
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
ent-cassadiene + [reduced NADPH-hemoprotein reductase] + O2
-
717875
Oryza sativa
?
-
-
-
?
ent-isokaurene + [reduced NADPH-hemoprotein reductase] + O2
-
717875
Oryza sativa
?
-
-
-
?
ent-kaurene + [reduced NADPH-hemoprotein reductase] + O2
-
717875
Oryza sativa
?
-
-
-
?
ent-pimaradiene + [reduced NADPH-hemoprotein reductase] + O2
-
717875
Oryza sativa
?
-
-
-
?
ent-sandaracopimaradien-3beta-ol + [reduced NADPH-hemoprotein reductase] + O2
-
717875
Oryza sativa
oryzalexin D + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
additional information
no reaction with syn-pimaradien-19-oic acid, syn-stemodene, syn-stemarene, or syn-labdatriene
717875
Oryza sativa
?
-
-
-
?
syn-pimaradiene + [reduced NADPH-hemoprotein reductase] + O2
-
717875
Oryza sativa
momilactone A + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
Synonyms
Synonyms
Commentary
Organism
CYP76M8
-
Oryza sativa
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.006
-
ent-cassadiene
pH and temperature not specified in the publication
Oryza sativa
0.006
-
syn-pimaradiene
pH and temperature not specified in the publication
Oryza sativa
0.009
-
ent-kaurene
pH and temperature not specified in the publication
Oryza sativa
0.009
-
ent-pimaradiene
pH and temperature not specified in the publication
Oryza sativa
0.011
-
ent-sandaracopimaradien-3beta-ol
pH and temperature not specified in the publication
Oryza sativa
0.015
-
ent-isokaurene
pH and temperature not specified in the publication
Oryza sativa
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in Escherichia coli C41 cells
Oryza sativa
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.002
-
syn-pimaradiene
pH and temperature not specified in the publication
Oryza sativa
0.004
-
ent-cassadiene
pH and temperature not specified in the publication
Oryza sativa
0.004
-
ent-pimaradiene
pH and temperature not specified in the publication
Oryza sativa
0.005
-
ent-kaurene
pH and temperature not specified in the publication
Oryza sativa
0.007
-
ent-sandaracopimaradien-3beta-ol
pH and temperature not specified in the publication
Oryza sativa
0.015
-
ent-isokaurene
pH and temperature not specified in the publication
Oryza sativa
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
ent-sandaracopimaradien-3beta-ol + [reduced NADPH-hemoprotein reductase] + O2
Oryza sativa
-
oryzalexin D + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
?
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
ent-cassadiene + [reduced NADPH-hemoprotein reductase] + O2
-
717875
Oryza sativa
?
-
-
-
?
ent-isokaurene + [reduced NADPH-hemoprotein reductase] + O2
-
717875
Oryza sativa
?
-
-
-
?
ent-kaurene + [reduced NADPH-hemoprotein reductase] + O2
-
717875
Oryza sativa
?
-
-
-
?
ent-pimaradiene + [reduced NADPH-hemoprotein reductase] + O2
-
717875
Oryza sativa
?
-
-
-
?
ent-sandaracopimaradien-3beta-ol + [reduced NADPH-hemoprotein reductase] + O2
-
717875
Oryza sativa
oryzalexin D + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
additional information
no reaction with syn-pimaradien-19-oic acid, syn-stemodene, syn-stemarene, or syn-labdatriene
717875
Oryza sativa
?
-
-
-
?
syn-pimaradiene + [reduced NADPH-hemoprotein reductase] + O2
-
717875
Oryza sativa
momilactone A + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.006
-
ent-cassadiene
pH and temperature not specified in the publication
Oryza sativa
0.006
-
syn-pimaradiene
pH and temperature not specified in the publication
Oryza sativa
0.009
-
ent-kaurene
pH and temperature not specified in the publication
Oryza sativa
0.009
-
ent-pimaradiene
pH and temperature not specified in the publication
Oryza sativa
0.011
-
ent-sandaracopimaradien-3beta-ol
pH and temperature not specified in the publication
Oryza sativa
0.015
-
ent-isokaurene
pH and temperature not specified in the publication
Oryza sativa
KCat/KM [mM/s]
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
1
-
ent-isokaurene
pH and temperature not specified in the publication
Oryza sativa
1.5
-
ent-cassadiene
pH and temperature not specified in the publication
Oryza sativa
1.6
-
ent-sandaracopimaradien-3beta-ol
pH and temperature not specified in the publication
Oryza sativa
1.8
-
ent-kaurene
pH and temperature not specified in the publication
Oryza sativa
2.3
-
ent-pimaradiene
pH and temperature not specified in the publication
Oryza sativa
2.6
-
syn-pimaradiene
pH and temperature not specified in the publication
Oryza sativa
KCat/KM [mM/s] (protein specific)
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
1
-
ent-isokaurene
pH and temperature not specified in the publication
Oryza sativa
1.5
-
ent-cassadiene
pH and temperature not specified in the publication
Oryza sativa
1.6
-
ent-sandaracopimaradien-3beta-ol
pH and temperature not specified in the publication
Oryza sativa
1.8
-
ent-kaurene
pH and temperature not specified in the publication
Oryza sativa
2.3
-
ent-pimaradiene
pH and temperature not specified in the publication
Oryza sativa
2.6
-
syn-pimaradiene
pH and temperature not specified in the publication
Oryza sativa
Other publictions for EC 1.14.14.123
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
729163
Wu
Picking sides: Distinct roles ...
Oryza sativa
Biochem. J.
454
209-216
2013
-
-
-
-
-
-
-
-
-
-
-
1
-
3
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
717875
Wang
Characterization of CYP76M5-8 ...
Oryza sativa
J. Biol. Chem.
287
6159-6168
2012
-
-
1
-
-
-
-
6
-
-
-
1
-
3
-
-
-
-
-
-
-
-
7
-
1
-
-
-
6
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
6
-
-
-
1
-
-
-
-
-
-
-
-
7
-
-
-
-
6
-
-
-
-
-
-
-
-
6
6