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Literature summary for 1.14.14.121 extracted from

  • Park, S.B.; Chun, J.H.; Ban, Y.W.; Han, J.Y.; Choi, Y.E.
    Alteration of Panax ginseng saponin composition by overexpression and RNA interference of the protopanaxadiol 6-hydroxylase gene (CYP716A53v2) (2016), J. Ginseng Res., 40, 47-54 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
gene CYP716A53v2, recombinant overexpression in Panx ginseng roots using the Agrobacterium tumefaciens strain GV3101 transformation method, expression under control of CaMV 35S promoter, subcloning in Escherichia coli strain DH5alpha Panax ginseng

Protein Variants

Protein Variants Comment Organism
additional information construction of transgenic Panax ginseng lines overexpressing CYP716A53v2 gene or silencing CYP716A53v2 gene via RNA interference (RNAi), analyis changes in ginsenoside profiles, overview. Production of protopanaxadiol- and protopanaxatriol-group ginsenosides can be altered by changing the expression of CYP716A53v2 in transgenic Panax ginseng. Changing the expression of CYP716A53v2 affects the downstream genes (glycosyltransferases) Panax ginseng

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
protopanaxadiol + [reduced NADPH-hemoprotein reductase] + O2 Panax ginseng
-
protopanaxatriol + [oxidized NADPH-hemoprotein reductase] + H2O
-
?

Organism

Organism UniProt Comment Textmining
Panax ginseng I7CT85
-
-

Source Tissue

Source Tissue Comment Organism Textmining
root
-
Panax ginseng
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
protopanaxadiol + [reduced NADPH-hemoprotein reductase] + O2
-
Panax ginseng protopanaxatriol + [oxidized NADPH-hemoprotein reductase] + H2O
-
?

Synonyms

Synonyms Comment Organism
CYP716A53v2
-
Panax ginseng

Cofactor

Cofactor Comment Organism Structure
cytochrome P450
-
Panax ginseng
NADPH-hemoprotein reductase A flavoprotein containing both FMN and FAD. This enzyme catalyses the transfer of electrons from NADPH, an obligatory two-electron donor, to microsomal P-450 monooxygenases, EC 1.14.14._ Panax ginseng

General Information

General Information Comment Organism
metabolism two cytochrome P450 (CYP) genes, CYP716A47 and CYP716A53v2, are critical for the production of protopanaxadiol and protopanaxatriol aglycones, respectively. CYP716A53v2 is a protopanaxadiol 6-hydroxylase that catalyzes protopanaxatriol production from protopanaxadiol in Panax ginseng, biosynthetic pathway for ginsenosides in Panax ginseng, overview Panax ginseng