Cloned (Comment) | Organism |
---|---|
gene PqD12H, expression of PqD12H in Saccharmoyces cerevisiae strain WAT21 from plasmid pAUR123-PqD12H, RT-PCR enzyme expression analysis, recombinant coexpression with dammarenediol synthase DS leads to increased accumulation of ginsenosides, i.e. protopanaxatriol protopanaxadiol. compared to control, both PqDS and PqD12H have higher accumulation of mRNA in the transgenic hairy roots and the increase ratio decreased slightly over culture time. Yeast with single recombinant vector pAUR123-PqD12H is unable to express protopanaxadiol without exogenous dammarenediol-II | Panax quinquefolius |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
dammarenediol-II + [reduced NADPH-hemoprotein reductase] + O2 | Panax quinquefolius | - |
protopanaxadiol + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Panax quinquefolius | K4K542 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
hairy root | - |
Panax quinquefolius | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
dammarenediol-II + [reduced NADPH-hemoprotein reductase] + O2 | - |
Panax quinquefolius | protopanaxadiol + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
PqD12H | - |
Panax quinquefolius |
protopanaxadiol synthase | - |
Panax quinquefolius |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
cytochrome P450 | - |
Panax quinquefolius | |
NADPH-hemoprotein reductase | A flavoprotein containing both FMN and FAD. This enzyme catalyses the transfer of electrons from NADPH, an obligatory two-electron donor, to microsomal P-450 monooxygenases, EC 1.14.14._ | Panax quinquefolius |