BRENDA - Enzyme Database
show all sequences of 1.14.14.117

Synthesis of 11-hydroxyl O-methylsterigmatocystin and the role of a cytochrome P-450 in the final step of aflatoxin biosynthesis

Udwary, D.W.; Casillas, L.K.; Townsend, C.A.; J. Am. Chem. Soc. 124, 5294-5303 (2002)

Data extracted from this reference:

Cloned(Commentary)
Cloned (Commentary)
Organism
expressed in Saccharomyces cerevisiae strain INVSc2
Aspergillus parasiticus
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
membrane
-
Aspergillus parasiticus
16020
-
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
60000
-
x * 60000, SDS-PAGE
Aspergillus parasiticus
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
8-O-methyldihydrosterigmatocystin + [reduced NADPH-hemoprotein reductase] + O2
Aspergillus parasiticus
-
aflatoxin B2 + methanol + CO2 + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
?
8-O-methyldihydrosterigmatocystin + [reduced NADPH-hemoprotein reductase] + O2
Aspergillus parasiticus SU-1
-
aflatoxin B2 + methanol + CO2 + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
?
8-O-methylsterigmatocystin + [reduced NADPH-hemoprotein reductase] + O2
Aspergillus parasiticus
-
aflatoxin B1 + 2 NADP+ + methanol + [oxidized NADPH-hemoprotein reductase] + H2O + CO2
-
-
?
8-O-methylsterigmatocystin + [reduced NADPH-hemoprotein reductase] + O2
Aspergillus parasiticus SU-1
-
aflatoxin B1 + 2 NADP+ + methanol + [oxidized NADPH-hemoprotein reductase] + H2O + CO2
-
-
?
Organism
Organism
UniProt
Commentary
Textmining
Aspergillus parasiticus
-
ATCC 56775
-
Aspergillus parasiticus SU-1
-
ATCC 56775
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
8-O-methyldihydrosterigmatocystin + [reduced NADPH-hemoprotein reductase] + O2
-
722440
Aspergillus parasiticus
aflatoxin B2 + methanol + CO2 + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
8-O-methyldihydrosterigmatocystin + [reduced NADPH-hemoprotein reductase] + O2
-
722440
Aspergillus parasiticus SU-1
aflatoxin B2 + methanol + CO2 + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
8-O-methylsterigmatocystin + [reduced NADPH-hemoprotein reductase] + O2
-
722440
Aspergillus parasiticus
aflatoxin B1 + 2 NADP+ + methanol + [oxidized NADPH-hemoprotein reductase] + H2O + CO2
-
-
-
?
8-O-methylsterigmatocystin + [reduced NADPH-hemoprotein reductase] + O2
-
722440
Aspergillus parasiticus SU-1
aflatoxin B1 + 2 NADP+ + methanol + [oxidized NADPH-hemoprotein reductase] + H2O + CO2
-
-
-
?
Subunits
Subunits
Commentary
Organism
?
x * 60000, SDS-PAGE
Aspergillus parasiticus
Synonyms
Synonyms
Commentary
Organism
ordA
-
Aspergillus parasiticus
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in Saccharomyces cerevisiae strain INVSc2
Aspergillus parasiticus
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
membrane
-
Aspergillus parasiticus
16020
-
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
60000
-
x * 60000, SDS-PAGE
Aspergillus parasiticus
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
8-O-methyldihydrosterigmatocystin + [reduced NADPH-hemoprotein reductase] + O2
Aspergillus parasiticus
-
aflatoxin B2 + methanol + CO2 + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
?
8-O-methyldihydrosterigmatocystin + [reduced NADPH-hemoprotein reductase] + O2
Aspergillus parasiticus SU-1
-
aflatoxin B2 + methanol + CO2 + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
?
8-O-methylsterigmatocystin + [reduced NADPH-hemoprotein reductase] + O2
Aspergillus parasiticus
-
aflatoxin B1 + 2 NADP+ + methanol + [oxidized NADPH-hemoprotein reductase] + H2O + CO2
-
-
?
8-O-methylsterigmatocystin + [reduced NADPH-hemoprotein reductase] + O2
Aspergillus parasiticus SU-1
-
aflatoxin B1 + 2 NADP+ + methanol + [oxidized NADPH-hemoprotein reductase] + H2O + CO2
-
-
?
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
8-O-methyldihydrosterigmatocystin + [reduced NADPH-hemoprotein reductase] + O2
-
722440
Aspergillus parasiticus
aflatoxin B2 + methanol + CO2 + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
8-O-methyldihydrosterigmatocystin + [reduced NADPH-hemoprotein reductase] + O2
-
722440
Aspergillus parasiticus SU-1
aflatoxin B2 + methanol + CO2 + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
8-O-methylsterigmatocystin + [reduced NADPH-hemoprotein reductase] + O2
-
722440
Aspergillus parasiticus
aflatoxin B1 + 2 NADP+ + methanol + [oxidized NADPH-hemoprotein reductase] + H2O + CO2
-
-
-
?
8-O-methylsterigmatocystin + [reduced NADPH-hemoprotein reductase] + O2
-
722440
Aspergillus parasiticus SU-1
aflatoxin B1 + 2 NADP+ + methanol + [oxidized NADPH-hemoprotein reductase] + H2O + CO2
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 60000, SDS-PAGE
Aspergillus parasiticus
Other publictions for EC 1.14.14.117
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
723750
Jamali
Expression of aflatoxin genes ...
Aspergillus flavus, Aspergillus flavus PFCC 101, Aspergillus flavus PFCC 116, Aspergillus flavus PFCC 137, Aspergillus flavus PFCC 176, Aspergillus flavus PFCC 209, Aspergillus flavus PFCC 247, Aspergillus flavus PFCC 267, Aspergillus flavus PFCC 309, Aspergillus parasiticus, Aspergillus parasiticus NRRL 2999, Aspergillus parasiticus PFCC 14
Res. Microbiol.
164
293-299
2013
-
-
-
-
-
-
-
-
-
-
-
-
-
12
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
722440
Udwary
Synthesis of 11-hydroxyl O-met ...
Aspergillus parasiticus, Aspergillus parasiticus SU-1
J. Am. Chem. Soc.
124
5294-5303
2002
-
-
1
-
-
-
-
-
1
-
1
4
-
4
-
-
-
-
-
-
-
-
4
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
1
4
-
-
-
-
-
-
-
-
4
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
721325
Yu
Characterization of the critic ...
Aspergillus parasiticus, Aspergillus parasiticus SRRC 143
Appl. Environ. Microbiol.
64
4834-4841
1998
-
-
1
-
3
-
-
-
-
-
-
4
-
2
-
-
-
-
-
1
-
-
4
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
3
-
-
-
-
-
-
-
-
4
-
-
-
-
-
1
-
-
4
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
721574
Bhatnagar
Enzymological evidence for sep ...
Aspergillus flavus, Aspergillus flavus SRRC 141, Aspergillus parasiticus, Aspergillus parasiticus SRRC 163
Biochemistry
30
4343-4350
1991
-
-
-
-
-
-
-
2
-
-
-
8
-
4
-
-
2
-
-
-
2
-
8
-
-
2
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
8
-
-
-
2
-
-
2
-
8
-
2
-
-
-
2
-
-
-
-
-
-
-
-
-