Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli DH10Bac cells and in Saccharomyces cerevisiae strain BJ2168 | Glycyrrhiza uralensis |
expressed in Saccharomyces cerevisiae strain BJ2168 that endogenously produces 11-oxo-beta-amyrin | Glycyrrhiza uralensis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
11-oxo-beta-amyrin + O2 + [reduced NADPH-hemoprotein reductase] | Glycyrrhiza uralensis | the enzyme mainly catalyzes the C-30 hydroxylation of 11-oxo-beta-amyrin to yield 30-hydroxy-11-oxo-beta-amyrin as the major product, accompanied by its isomers as minor products | 30-hydroxy-11-oxo-beta-amyrin + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Glycyrrhiza uralensis | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
colon | - |
Glycyrrhiza uralensis | - |
additional information | not detected in leaves | Glycyrrhiza uralensis | - |
root | - |
Glycyrrhiza uralensis | - |
stem | - |
Glycyrrhiza uralensis | - |
stolon | - |
Glycyrrhiza uralensis | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
11-oxo-beta-amyrin + O2 + [reduced NADPH-hemoprotein reductase] | single oxidation reaction catalyzed by CYP72A154 | Glycyrrhiza uralensis | 30-hydroxy-11-oxo-beta-amyrin + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
11-oxo-beta-amyrin + O2 + [reduced NADPH-hemoprotein reductase] | the enzyme mainly catalyzes the C-30 hydroxylation of 11-oxo-beta-amyrin to yield 30-hydroxy-11-oxo-beta-amyrin as the major product, accompanied by its isomers as minor products | Glycyrrhiza uralensis | 30-hydroxy-11-oxo-beta-amyrin + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
30-hydroxy-11-oxo-beta-amyrin + O2 + [reduced NADPH-hemoprotein reductase] | - |
Glycyrrhiza uralensis | glycyrrhetaldehyde + [oxidized NADPH-hemoprotein reductase] + 2 H2O | - |
? | |
30-hydroxy-11-oxo-beta-amyrin + O2 + [reduced NADPH-hemoprotein reductase] | single oxidation reaction catalyzed by CYP72A154 | Glycyrrhiza uralensis | glycyrrhetaldehyde + [oxidized NADPH-hemoprotein reductase] + 2 H2O | - |
? | |
glycyrrhetaldehyde + O2 + [reduced NADPH-hemoprotein reductase] | - |
Glycyrrhiza uralensis | glycyrrhetinate + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
CYP72A154 | - |
Glycyrrhiza uralensis |
CYP88D6 | - |
Glycyrrhiza uralensis |
General Information | Comment | Organism |
---|---|---|
metabolism | the beta-amyrin 11-oxidase gene CYP88D6 is the initial P450 gene in glycyrrhizin biosynthesis. CYP72A154 is responsible for C-30 oxidation of beta-amyrin in the glycyrrhizin pathway | Glycyrrhiza uralensis |
metabolism | the enzyme is involved in the biosynthesis of glycyrrhizin | Glycyrrhiza uralensis |