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Literature summary for 1.14.14.113 extracted from

  • Harada, H.; Shindo, K.; Iki, K.; Teraoka, A.; Okamoto, S.; Yu, F.; Hattan, J.; Utsumi, R.; Misawa, N.
    Efficient functional analysis system for cyanobacterial or plant cytochromes P450 involved in sesquiterpene biosynthesis (2011), Appl. Microbiol. Biotechnol., 90, 467-476.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
CYP71BA1 expression in Escherichia coli. Co-expression with germacrene A hydroxylase P450NSor CYP110C1 from Nostoc sp. PCC 7120 and the Arabidopsis thaliana ATR2 as well as two corresponding enzymes derived from Zingiber officinale, named ZoRED1 and ZoRED2 from one singel expression plasmid, construction, overview. The co-expression enhances CYP71BA1 expression levels by 1.5-2fold comapred to single heterologous expression Zingiber zerumbet

Protein Variants

Protein Variants Comment Organism
additional information reconstruction of the isoprenoid biosynthetic pathway in Escherichia coli, overview Zingiber zerumbet

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ cytochrome P450 enzyme Zingiber zerumbet

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
alpha-humulene + O2 + [reduced NADPH-hemoprotein reductase] Zingiber zerumbet
-
10-hydroxy-alpha-humulene + [oxidized NADPH-hemoprotein reductase] + H2O
-
?

Organism

Organism UniProt Comment Textmining
Zingiber zerumbet
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
alpha-humulene + O2 + [reduced NADPH-hemoprotein reductase]
-
Zingiber zerumbet 10-hydroxy-alpha-humulene + [oxidized NADPH-hemoprotein reductase] + H2O
-
?

Synonyms

Synonyms Comment Organism
alpha-humulene-8-hydroxylase
-
Zingiber zerumbet
CYP71BA1
-
Zingiber zerumbet

Cofactor

Cofactor Comment Organism Structure
cytochrome P450
-
Zingiber zerumbet

General Information

General Information Comment Organism
evolution the enzyme belongs to the cytochrome P450 monooxygenases Zingiber zerumbet