Cloned (Comment) | Organism |
---|---|
CYP71BA1 expression in Escherichia coli. Co-expression with germacrene A hydroxylase P450NSor CYP110C1 from Nostoc sp. PCC 7120 and the Arabidopsis thaliana ATR2 as well as two corresponding enzymes derived from Zingiber officinale, named ZoRED1 and ZoRED2 from one singel expression plasmid, construction, overview. The co-expression enhances CYP71BA1 expression levels by 1.5-2fold comapred to single heterologous expression | Zingiber zerumbet |
Protein Variants | Comment | Organism |
---|---|---|
additional information | reconstruction of the isoprenoid biosynthetic pathway in Escherichia coli, overview | Zingiber zerumbet |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | cytochrome P450 enzyme | Zingiber zerumbet |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
alpha-humulene + O2 + [reduced NADPH-hemoprotein reductase] | Zingiber zerumbet | - |
10-hydroxy-alpha-humulene + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Zingiber zerumbet | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
alpha-humulene + O2 + [reduced NADPH-hemoprotein reductase] | - |
Zingiber zerumbet | 10-hydroxy-alpha-humulene + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
alpha-humulene-8-hydroxylase | - |
Zingiber zerumbet |
CYP71BA1 | - |
Zingiber zerumbet |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
cytochrome P450 | - |
Zingiber zerumbet |
General Information | Comment | Organism |
---|---|---|
evolution | the enzyme belongs to the cytochrome P450 monooxygenases | Zingiber zerumbet |