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show all sequences of 1.14.14.111

CYP99A3: Functional identification of a diterpene oxidase from the momilactone biosynthetic gene cluster in rice

Wang, Q.; Hillwig, M.; Peters, R.; Plant J. 65, 87-95 (2011)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expressed in Escherichia coli C41 cells
Oryza sativa
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.002
-
syn-pimara-7,15-diene
pH and temperature not specified in the publication
Oryza sativa
0.009
-
syn-stemod-13(17)-ene
pH and temperature not specified in the publication
Oryza sativa
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Oryza sativa
Q0JF01
cultivar Nipponbare
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
additional information
CYP99A3 catalyzes consecutive oxidations of the C19 methyl group of the momilactone precursor syn-pimara-7,15-diene to form, sequentially, syn-pimaradien-19-ol, syn-pimaradien-19-al, and syn-pimaradien-19-oate
718217
Oryza sativa
?
-
-
-
-
syn-pimara-7,15-diene + [reduced NADPH-hemoprotein reductase] + O2
-
718217
Oryza sativa
syn-pimaradien-19-ol + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
syn-pimaradien-19-al + [reduced NADPH-hemoprotein reductase] + O2
-
718217
Oryza sativa
syn-pimaradien-19-oate + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
syn-pimaradien-19-ol + [reduced NADPH-hemoprotein reductase] + O2
-
718217
Oryza sativa
syn-pimaradien-19-al + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
syn-stemod-13(17)-ene + [reduced NADPH-hemoprotein reductase] + O2
about 4fold lower catalytic efficiency than with syn-pimaradiene, oxidation occurs at C19
718217
Oryza sativa
syn-stemoden-19-ol + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
syn-stemoden-19-al + [reduced NADPH-hemoprotein reductase] + O2
about 4fold lower catalytic efficiency than with syn-pimaradiene, oxidation occurs at C19
718217
Oryza sativa
syn-stemoden-19-oate + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
syn-stemoden-19-ol + [reduced NADPH-hemoprotein reductase] + O2
about 4fold lower catalytic efficiency than with syn-pimaradiene, oxidation occurs at C19
718217
Oryza sativa
syn-stemoden-19-al + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
46
-
syn-pimara-7,15-diene
pH and temperature not specified in the publication
Oryza sativa
49
-
syn-stemod-13(17)-ene
pH and temperature not specified in the publication
Oryza sativa
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in Escherichia coli C41 cells
Oryza sativa
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.002
-
syn-pimara-7,15-diene
pH and temperature not specified in the publication
Oryza sativa
0.009
-
syn-stemod-13(17)-ene
pH and temperature not specified in the publication
Oryza sativa
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
additional information
CYP99A3 catalyzes consecutive oxidations of the C19 methyl group of the momilactone precursor syn-pimara-7,15-diene to form, sequentially, syn-pimaradien-19-ol, syn-pimaradien-19-al, and syn-pimaradien-19-oate
718217
Oryza sativa
?
-
-
-
-
syn-pimara-7,15-diene + [reduced NADPH-hemoprotein reductase] + O2
-
718217
Oryza sativa
syn-pimaradien-19-ol + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
syn-pimaradien-19-al + [reduced NADPH-hemoprotein reductase] + O2
-
718217
Oryza sativa
syn-pimaradien-19-oate + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
syn-pimaradien-19-ol + [reduced NADPH-hemoprotein reductase] + O2
-
718217
Oryza sativa
syn-pimaradien-19-al + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
syn-stemod-13(17)-ene + [reduced NADPH-hemoprotein reductase] + O2
about 4fold lower catalytic efficiency than with syn-pimaradiene, oxidation occurs at C19
718217
Oryza sativa
syn-stemoden-19-ol + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
syn-stemoden-19-al + [reduced NADPH-hemoprotein reductase] + O2
about 4fold lower catalytic efficiency than with syn-pimaradiene, oxidation occurs at C19
718217
Oryza sativa
syn-stemoden-19-oate + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
syn-stemoden-19-ol + [reduced NADPH-hemoprotein reductase] + O2
about 4fold lower catalytic efficiency than with syn-pimaradiene, oxidation occurs at C19
718217
Oryza sativa
syn-stemoden-19-al + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
46
-
syn-pimara-7,15-diene
pH and temperature not specified in the publication
Oryza sativa
49
-
syn-stemod-13(17)-ene
pH and temperature not specified in the publication
Oryza sativa
General Information
General Information
Commentary
Organism
physiological function
CYP99A3 acts as a multifunctional diterpene oxidase in momilactone biosynthesis
Oryza sativa
General Information (protein specific)
General Information
Commentary
Organism
physiological function
CYP99A3 acts as a multifunctional diterpene oxidase in momilactone biosynthesis
Oryza sativa
KCat/KM [mM/s]
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
5000
-
syn-stemod-13(17)-ene
pH and temperature not specified in the publication
Oryza sativa
23000
-
syn-pimara-7,15-diene
pH and temperature not specified in the publication
Oryza sativa
KCat/KM [mM/s] (protein specific)
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
5000
-
syn-stemod-13(17)-ene
pH and temperature not specified in the publication
Oryza sativa
23000
-
syn-pimara-7,15-diene
pH and temperature not specified in the publication
Oryza sativa
Other publictions for EC 1.14.14.111
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
718217
Wang
CYP99A3: Functional identifica ...
Oryza sativa
Plant J.
65
87-95
2011
-
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1
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2
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4
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7
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2
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1
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2
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7
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2
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1
1
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2
2
680848
Shimura
Identification of a biosynthet ...
Oryza sativa
J. Biol. Chem.
282
34013-34018
2007
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1
-
-
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-
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-
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3
-
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-
-
-
-
-
-
1
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-
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-
-
-
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-
-
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1
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-
-
-
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-
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-
-
-
-
-
-
-
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1
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-
-
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2
2
-
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