Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Rhodococcus sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
3-chlorostyrene | less than 30% residual activity with 25 mM 3-chlorostyrene; less than 30% residual activity with 25 mM 3-chlorostyrene | Rhodococcus sp. | |
Benzene | about 50% residual activity with 25 mM benzene; about 50% residual activity with 25 mM benzene | Rhodococcus sp. | |
additional information | not inhibited by 1-hexene; not inhibited by 1-hexene | Rhodococcus sp. | |
Styrene | less than 40% residual activity with 5 mM styrene; less than 40% residual activity with 5 mM styrene | Rhodococcus sp. | |
styrene oxide | marked inhibition; marked inhibition | Rhodococcus sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
45500 | - |
1 * 45500, calculated from amino acid sequence | Rhodococcus sp. |
47300 | - |
1 * 47300, calculated from amino acid sequence | Rhodococcus sp. |
59000 | - |
gel filtration | Rhodococcus sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
styrene + FADH2 + O2 | Rhodococcus sp. | - |
(S)-styrene oxide + FAD + H2O | - |
? | |
styrene + FADH2 + O2 | Rhodococcus sp. ST-5 | - |
(S)-styrene oxide + FAD + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rhodococcus sp. | G3XEX2 | - |
- |
Rhodococcus sp. | G3XEX5 | - |
- |
Rhodococcus sp. ST-5 | G3XEX5 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni-Sepharose column chromatography and MonoQ column chromatography | Rhodococcus sp. |
Toyopearl SuperQ-650 M column chromatography, Ni-Sepharose column chromatography, MonoQ column chromatography and Superdex 200 gel filtration | Rhodococcus sp. |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.0026 | - |
cell-free extract, at pH 7.0 and 30°C | Rhodococcus sp. |
0.0114 | - |
cell-free extract, at pH 7.0 and °C | Rhodococcus sp. |
0.0255 | - |
after 2.2fold purification, at pH 7.0 and 30°C | Rhodococcus sp. |
0.0304 | - |
after 11.7fold purification, at pH 7.0 and 30°C | Rhodococcus sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-hexene + FADH2 + O2 | - |
Rhodococcus sp. | ? | - |
? | |
1-hexene + FADH2 + O2 | highest activity | Rhodococcus sp. | ? | - |
? | |
1-hexene + FADH2 + O2 | - |
Rhodococcus sp. ST-5 | ? | - |
? | |
1-octene + FADH2 + O2 | - |
Rhodococcus sp. | ? | - |
? | |
1-octene + FADH2 + O2 | - |
Rhodococcus sp. ST-5 | ? | - |
? | |
2-chlorostyrene + FADH2 + O2 | - |
Rhodococcus sp. | ? | - |
? | |
2-chlorostyrene + FADH2 + O2 | - |
Rhodococcus sp. ST-5 | ? | - |
? | |
3-chlorostyrene + FADH2 + O2 | - |
Rhodococcus sp. | ? | - |
? | |
3-chlorostyrene + FADH2 + O2 | - |
Rhodococcus sp. ST-5 | ? | - |
? | |
4-bromostyrene + FADH2 + O2 | - |
Rhodococcus sp. | ? | - |
? | |
4-chlorostyrene + FADH2 + O2 | - |
Rhodococcus sp. | ? | - |
? | |
4-chlorostyrene + FADH2 + O2 | highest activity | Rhodococcus sp. | ? | - |
? | |
4-fluorostyrene + FADH2 + O2 | - |
Rhodococcus sp. | ? | - |
? | |
allylbenzene + FADH2 + O2 | - |
Rhodococcus sp. | ? | - |
? | |
indole + FADH2 + O2 | - |
Rhodococcus sp. | ? | - |
? | |
styrene + FADH2 + O2 | - |
Rhodococcus sp. | (S)-styrene oxide + FAD + H2O | - |
? | |
styrene + FADH2 + O2 | - |
Rhodococcus sp. ST-5 | (S)-styrene oxide + FAD + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 45500, calculated from amino acid sequence | Rhodococcus sp. |
monomer | 1 * 47300, calculated from amino acid sequence | Rhodococcus sp. |
Synonyms | Comment | Organism |
---|---|---|
SmoA | - |
Rhodococcus sp. |
StyA | - |
Rhodococcus sp. |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | the enzyme favors FAD and FMN over riboflavin | Rhodococcus sp. | |
FMN | the enzyme favors FAD and FMN over riboflavin | Rhodococcus sp. |