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Literature summary for 1.14.14.11 extracted from

  • Toda, H.; Imae, R.; Komio, T.; Itoh, N.
    Expression and characterization of styrene monooxygenases of Rhodococcus sp. ST-5 and ST-10 for synthesizing enantiopure (S)-epoxides (2012), Appl. Microbiol. Biotechnol., 96, 407-418.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Rhodococcus sp.

Inhibitors

Inhibitors Comment Organism Structure
3-chlorostyrene less than 30% residual activity with 25 mM 3-chlorostyrene; less than 30% residual activity with 25 mM 3-chlorostyrene Rhodococcus sp.
Benzene about 50% residual activity with 25 mM benzene; about 50% residual activity with 25 mM benzene Rhodococcus sp.
additional information not inhibited by 1-hexene; not inhibited by 1-hexene Rhodococcus sp.
Styrene less than 40% residual activity with 5 mM styrene; less than 40% residual activity with 5 mM styrene Rhodococcus sp.
styrene oxide marked inhibition; marked inhibition Rhodococcus sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
45500
-
1 * 45500, calculated from amino acid sequence Rhodococcus sp.
47300
-
1 * 47300, calculated from amino acid sequence Rhodococcus sp.
59000
-
gel filtration Rhodococcus sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
styrene + FADH2 + O2 Rhodococcus sp.
-
(S)-styrene oxide + FAD + H2O
-
?
styrene + FADH2 + O2 Rhodococcus sp. ST-5
-
(S)-styrene oxide + FAD + H2O
-
?

Organism

Organism UniProt Comment Textmining
Rhodococcus sp. G3XEX2
-
-
Rhodococcus sp. G3XEX5
-
-
Rhodococcus sp. ST-5 G3XEX5
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-Sepharose column chromatography and MonoQ column chromatography Rhodococcus sp.
Toyopearl SuperQ-650 M column chromatography, Ni-Sepharose column chromatography, MonoQ column chromatography and Superdex 200 gel filtration Rhodococcus sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0026
-
cell-free extract, at pH 7.0 and 30°C Rhodococcus sp.
0.0114
-
cell-free extract, at pH 7.0 and °C Rhodococcus sp.
0.0255
-
after 2.2fold purification, at pH 7.0 and 30°C Rhodococcus sp.
0.0304
-
after 11.7fold purification, at pH 7.0 and 30°C Rhodococcus sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-hexene + FADH2 + O2
-
Rhodococcus sp. ?
-
?
1-hexene + FADH2 + O2 highest activity Rhodococcus sp. ?
-
?
1-hexene + FADH2 + O2
-
Rhodococcus sp. ST-5 ?
-
?
1-octene + FADH2 + O2
-
Rhodococcus sp. ?
-
?
1-octene + FADH2 + O2
-
Rhodococcus sp. ST-5 ?
-
?
2-chlorostyrene + FADH2 + O2
-
Rhodococcus sp. ?
-
?
2-chlorostyrene + FADH2 + O2
-
Rhodococcus sp. ST-5 ?
-
?
3-chlorostyrene + FADH2 + O2
-
Rhodococcus sp. ?
-
?
3-chlorostyrene + FADH2 + O2
-
Rhodococcus sp. ST-5 ?
-
?
4-bromostyrene + FADH2 + O2
-
Rhodococcus sp. ?
-
?
4-chlorostyrene + FADH2 + O2
-
Rhodococcus sp. ?
-
?
4-chlorostyrene + FADH2 + O2 highest activity Rhodococcus sp. ?
-
?
4-fluorostyrene + FADH2 + O2
-
Rhodococcus sp. ?
-
?
allylbenzene + FADH2 + O2
-
Rhodococcus sp. ?
-
?
indole + FADH2 + O2
-
Rhodococcus sp. ?
-
?
styrene + FADH2 + O2
-
Rhodococcus sp. (S)-styrene oxide + FAD + H2O
-
?
styrene + FADH2 + O2
-
Rhodococcus sp. ST-5 (S)-styrene oxide + FAD + H2O
-
?

Subunits

Subunits Comment Organism
monomer 1 * 45500, calculated from amino acid sequence Rhodococcus sp.
monomer 1 * 47300, calculated from amino acid sequence Rhodococcus sp.

Synonyms

Synonyms Comment Organism
SmoA
-
Rhodococcus sp.
StyA
-
Rhodococcus sp.

Cofactor

Cofactor Comment Organism Structure
FAD the enzyme favors FAD and FMN over riboflavin Rhodococcus sp.
FMN the enzyme favors FAD and FMN over riboflavin Rhodococcus sp.