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Literature summary for 1.14.14.108 extracted from

  • McGhie, E.J.; Littlechild, J.A.
    The purification and crystallization of 2,5-diketocamphane 1,2-monooxygenase and 3,6-diketocamphane 1,6-monooxygenase from Pseudomonas putida NCIMB 10007 (1996), Biochem. Soc. Trans., 24, 29S.
    View publication on PubMed
Search for more literature for 1.14.14.108

Crystallization (Commentary)

Crystallization (Comment) Organism
crystal clusters, grown in 0.2 M CaCl2, 0.1 M HEPES and 30% PEG 400, X-ray analysis Pseudomonas putida

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
additional information
-
 enzyme consists of 2 components: 1 oxygenating and 1 NADH dehydrogenase Pseudomonas putida
36000
-
 NADH dehydrogenase component, SDS-PAGE Pseudomonas putida
39000 40000 oxygenating component, gel filtration Pseudomonas putida

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(+)-bornane-2,5-dione + FMNH2 + O2 Pseudomonas putida inducible by growth on racemic camphor ?
-
 ?
(+)-bornane-2,5-dione + FMNH2 + O2 Pseudomonas putida NCIMB 10007 inducible by growth on racemic camphor ?
-
 ?

Organism

Organism UniProt Comment Textmining
Pseudomonas putida
-
-
-
Pseudomonas putida NCIMB 10007
-
-
-

Purification (Commentary)

Purification (Comment) Organism
both components Pseudomonas putida

Reaction

Reaction Comment Organism Reaction ID
(+)-bornane-2,5-dione + FMNH2 + O2 = (+)-5-oxo-1,2-campholide + FMN + H2O mechanism Pseudomonas putida
a
(+)-bornane-2,5-dione + FMNH2 + O2 = (+)-5-oxo-1,2-campholide + FMN + H2O belongs to group of Bayer-Villiger monooxygenases of the NADH plus FMN-dependent type 2 enzymes Pseudomonas putida
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(+)-bornane-2,5-dione + FMNH2 + O2 i.e. 2,5-diketocamphane Pseudomonas putida 3,4,4-trimethyl-5-carboxy-methyl-DELTA2-cyclopentenone + FMN + H2O product is an unstable lactone-intermediate and forms spontaneously 2-oxo-DELTA3-4,5,5-trimethylcyclopentenyl acetic acid ?
(+)-bornane-2,5-dione + FMNH2 + O2 i.e. 2,5-diketocamphane Pseudomonas putida NCIMB 10007 3,4,4-trimethyl-5-carboxy-methyl-DELTA2-cyclopentenone + FMN + H2O product is an unstable lactone-intermediate and forms spontaneously 2-oxo-DELTA3-4,5,5-trimethylcyclopentenyl acetic acid ?
(+)-bornane-2,5-dione + FMNH2 + O2 inducible by growth on racemic camphor Pseudomonas putida ?
-
 ?
(+)-bornane-2,5-dione + FMNH2 + O2 inducible by growth on racemic camphor Pseudomonas putida NCIMB 10007 ?
-
 ?
(+)-bornanone + FMNH2 + O2
-
 Pseudomonas putida 1,2-campholide + FMN + H2O
-
 ?
(+)-bornanone + FMNH2 + O2
-
 Pseudomonas putida NCIMB 10007 1,2-campholide + FMN + H2O
-
 ?
additional information rubredoxin not mentioned Pseudomonas putida ?
-
 ?
additional information rubredoxin not mentioned Pseudomonas putida NCIMB 10007 ?
-
 ?

Subunits

Subunits Comment Organism
More enzyme consists of 2 components: 1 oxygenating and 1 NADH dehydrogenase Pseudomonas putida