Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | - |
Pseudomonas putida | 5737 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | no Fe2+ in dehydrogenase | Pseudomonas putida |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
additional information | - |
multi-component enzyme, consists of FMN-reductase and oxygenase compound | Pseudomonas putida |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas putida | - |
wild type strain C1B (PpG1) | - |
Purification (Comment) | Organism |
---|---|
- |
Pseudomonas putida |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
(+)-bornane-2,5-dione + FMNH2 + O2 = (+)-5-oxo-1,2-campholide + FMN + H2O | mechanism | Pseudomonas putida |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.43 | - |
substrate (+)-camphor, purified, reconstituted multimeric enzyme complex | Pseudomonas putida |
0.81 | - |
substrate diketocamphane, purified enzyme | Pseudomonas putida |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(+)-bornane-2,5-dione + FMNH2 + O2 | flavin reduction during reaction is reversible | Pseudomonas putida | 3,4,4-trimethyl-5-carboxy-methyl-DELTA2-cyclopentenone + FMN + H2O | i.e. cyclopentenoic acid | ? | |
(+)-bornane-2,5-dione + FMNH2 + O2 | i.e. 2,5-diketocamphane | Pseudomonas putida | 3,4,4-trimethyl-5-carboxy-methyl-DELTA2-cyclopentenone + FMN + H2O | i.e. cyclopentenoic acid | ? | |
(+)-bornanone + FMNH2 + O2 | (+)-camphor | Pseudomonas putida | 1,2-campholide + FMN + H2O | - |
? | |
additional information | rubredoxin not mentioned | Pseudomonas putida | ? | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Pseudomonas putida |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | - |
Pseudomonas putida |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.2 | - |
- |
Pseudomonas putida |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | - |
Pseudomonas putida | |
FMN | - |
Pseudomonas putida |