Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2,2'-bipyridine | dissociates FMN from oxygenating enzyme component | Pseudomonas putida | |
p-hydroxymercuribenzoate | oxygenase coupled to NADH oxidase | Pseudomonas putida |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | - |
Pseudomonas putida | 5737 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | no Fe2+ in dehydrogenase | Pseudomonas putida |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
additional information | - |
2 electrophoretic components, PAGE at pH 8.9 and higher | Pseudomonas putida |
37000 | - |
2 * 37000, oxygenating component E1, SDS-PAGE | Pseudomonas putida |
76000 | - |
oxygenating component E1, equilibrium ultracentrifugation | Pseudomonas putida |
120000 | - |
- |
Pseudomonas putida |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas putida | - |
- |
- |
Pseudomonas putida C1 / ATCC 17453 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Pseudomonas putida |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
3.56 | - |
oxygenating component | Pseudomonas putida |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(+)-bornane-2,5-dione + FMNH2 + O2 | i.e. 2,5-diketocamphane | Pseudomonas putida | 3,4,4-trimethyl-5-carboxy-methyl-DELTA2-cyclopentenone + FMN + H2O | i.e. cyclopentenoic acid | ? | |
(+)-bornane-2,5-dione + FMNH2 + O2 | i.e. 2,5-diketocamphane | Pseudomonas putida C1 / ATCC 17453 | 3,4,4-trimethyl-5-carboxy-methyl-DELTA2-cyclopentenone + FMN + H2O | i.e. cyclopentenoic acid | ? | |
(+)-bornanone + FMNH2 + O2 | (+)-camphor | Pseudomonas putida | 1,2-campholide + FMN + H2O | - |
? | |
(+)-bornanone + FMNH2 + O2 | (+)-camphor | Pseudomonas putida C1 / ATCC 17453 | 1,2-campholide + FMN + H2O | - |
? | |
additional information | rubredoxin not mentioned | Pseudomonas putida | ? | - |
? | |
additional information | rubredoxin not mentioned | Pseudomonas putida C1 / ATCC 17453 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 37000, oxygenating component E1, SDS-PAGE | Pseudomonas putida |
More | 2 electrophoretic components, PAGE at pH 8.9 and higher | Pseudomonas putida |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Pseudomonas putida |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.2 | - |
- |
Pseudomonas putida |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FMN | dialyzable, not covalently bound | Pseudomonas putida |