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Literature summary for 1.14.14.108 extracted from

  • Conrad, H.E.; DuBus, R.; Gunsalus, I.C.
    An enzyme system for cyclic ketone lactonization (1961), Biochem. Biophys. Res. Commun., 6, 293-297.
    View publication on PubMed
Search for more literature for 1.14.14.108

Activating Compound

Activating Compound Comment Organism Structure
additional information boiled cell-free extract, activation, replaces FMN in the assay Pseudomonas putida

Inhibitors

Inhibitors Comment Organism Structure
2,2'-bipyridine inhibition of the whole enzyme complex, but not of the components alone; reactivation by Fe2+, not by Co2+, Cu2+, Mn2+, Mg2+, Zn2+, Fe3+ Pseudomonas putida

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
 Pseudomonas putida 5737
-

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ requirement Pseudomonas putida
Fe2+ no Fe2+ in dehydrogenase Pseudomonas putida

Organism

Organism UniProt Comment Textmining
Pseudomonas putida
-
 wild type strain C1B (PpG1)
-

Purification (Commentary)

Purification (Comment) Organism
component E1, FMN-reductase Pseudomonas putida
component E2, lactonizing enzyme Pseudomonas putida

Reaction

Reaction Comment Organism Reaction ID
(+)-bornane-2,5-dione + FMNH2 + O2 = (+)-5-oxo-1,2-campholide + FMN + H2O mechanism Pseudomonas putida
a

Renatured (Commentary)

Renatured (Comment) Organism
inactive enzyme after dialysis can be reactivated by Fe2+ at 0.1 mM, Fe3+, Co2+, Zn2+, Mn2+, Mg2+, and Cu2+ are not usefull for reactivation Pseudomonas putida

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.67
-
 component E2, lactonizing Pseudomonas putida
38.3
-
 component E1, FMN-reductase Pseudomonas putida

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(+)-bornane-2,5-dione + FMNH2 + O2 i.e. 2,5-diketocamphane Pseudomonas putida 3,4,4-trimethyl-5-carboxy-methyl-DELTA2-cyclopentenone + FMN + H2O i.e. cyclopentenoic acid ?
additional information rubredoxin not mentioned Pseudomonas putida ?
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Pseudomonas putida

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.2
-
-
 Pseudomonas putida

Cofactor

Cofactor Comment Organism Structure
FAD activation, 30% activity of FMN Pseudomonas putida
FMN both enzymatic steps require FMN, not replaceable by GSH, Cys, sodium borohydride or ascorbate Pseudomonas putida