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Literature summary for 1.14.14.107 extracted from

  • Kai, K.; Kasa, S.; Sakamoto, M.; Aoki, N.; Watabe, G.; Yuasa, T.; Iwaya-Inoue, M.; Ishibashi, Y.
    Role of reactive oxygen species produced by NADPH oxidase in gibberellin biosynthesis during barley seed germination (2016), Plant Signal. Behav., 11, e1180492 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
gene HvKAO1, quantitative RT-PCR enzyme expression analysis Hordeum vulgare

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ent-kaur-16-en-19-oate + 3 [reduced NADPH-hemoprotein reductase] + 3 O2 Hordeum vulgare overall reaction gibberellin A12 + 3 [oxidized NADPH-hemoprotein reductase] + 4 H2O
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Organism

Organism UniProt Comment Textmining
Hordeum vulgare Q9AXH9 cv. Himalaya
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Source Tissue

Source Tissue Comment Organism Textmining
embryo
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Hordeum vulgare
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seed
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Hordeum vulgare
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ent-kaur-16-en-19-oate + 3 [reduced NADPH-hemoprotein reductase] + 3 O2 overall reaction Hordeum vulgare gibberellin A12 + 3 [oxidized NADPH-hemoprotein reductase] + 4 H2O
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?

Synonyms

Synonyms Comment Organism
ent-kaurenoic acid oxidase 1 UniProt Hordeum vulgare
HvKAO1
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Hordeum vulgare
KAO
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Hordeum vulgare

Cofactor

Cofactor Comment Organism Structure
cytochrome P-450
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Hordeum vulgare
NADPH-hemoprotein reductase A flavoprotein containing both FMN and FAD. This enzyme catalyses the transfer of electrons from NADPH, an obligatory two-electron donor, to microsomal P-450 monooxygenases, EC 1.14.14._ Hordeum vulgare

Expression

Organism Comment Expression
Hordeum vulgare diphenyleneiodonium reduces the expression of HvKAO1, about 20% at 1 mM down
Hordeum vulgare exogenous H2O2 promotes the expression of HvKAO1 in barley embryos up

General Information

General Information Comment Organism
physiological function reactive oxygen species (ROS), produced by NADPH oxidase, are involved in gibberellin biosynthesis through the regulation of ent-kaurenoic acid oxidase HvKAO1 and GA3-oxidase HvGA3ox1, relationship between ROS and gibberellin (GA) biosynthesis enzymes, overview Hordeum vulgare