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Literature summary for 1.14.14.106 extracted from

  • Jennewein, S.; Park, H.; DeJong, J.M.; Long, R.M.; Bollon, A.P.; Croteau, R.B.
    Coexpression in yeast of Taxus cytochrome P450 reductase with cytochrome P450 oxygenases involved in taxol biosynthesis (2005), Biotechnol. Bioeng., 89, 588-598.
    View publication on PubMed
Search for more literature for 1.14.14.106

Cloned(Commentary)

Cloned (Comment) Organism
functional co-expression with taxoid 10beta-hydroxylase in Saccharomyces cerevisiae strain YPH499, subcloning in Escherichia coli Taxus cuspidata

Localization

Localization Comment Organism GeneOntology No. Textmining
endoplasmic reticulum
-
 Taxus cuspidata 5783
-
microsome
-
 Taxus cuspidata
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ heme iron Taxus cuspidata

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
taxadien-5alpha-yl acetate + [reduced NADPH-hemoprotein reductase] + O2 Taxus cuspidata the enzyme is involved in the taxol biosynthetic pathway, overview taxa-4(20),11(12)-dien-5alpha-yl-acetyl-13alpha-ol + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Taxus cuspidata
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Taxus cuspidata

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
taxadien-5alpha-yl acetate + [reduced NADPH-hemoprotein reductase] + O2 the enzyme is involved in the taxol biosynthetic pathway, overview Taxus cuspidata taxa-4(20),11(12)-dien-5alpha-yl-acetyl-13alpha-ol + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
taxadien-5alpha-yl acetate + [reduced NADPH-hemoprotein reductase] + O2
-
 Taxus cuspidata taxa-4(20),11-dien-5alpha-yl-acetyl-13alpha-ol + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?

Synonyms

Synonyms Comment Organism
cytochrome P450 taxoid 13alpha-hydroxylase
-
 Taxus cuspidata
taxoid 13alpha-hydroxylase
-
 Taxus cuspidata

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.7
-
 assay at Taxus cuspidata

Cofactor

Cofactor Comment Organism Structure
cytochrome P-450
-
 Taxus cuspidata